##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AflII AgeI Aor51HI
AscI AseI AsnI Asp718I BclI
BfrI BsaBI BsiWI BspDI BspHI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII KpnI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI PvuI RcaI
RsrII SalI ScaI SfuI SnaBI
SpeI SphI SspI SwaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3564 GTAGAC GT^[AC][GT]AC
AccIII 1145 TCCGGA T^CCGGA
AclI 662 AACGTT AA^CGTT
AhdI 3567 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1377 GTGCAC G^TGCAC
ApaLI 1377 GTGCAC G^TGCAC
Asp700I 1600 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3567 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 433 GTATCC GTATCC(6/5)
BglII 3485 AGATCT A^GATCT
BseAI 1145 TCCGGA T^CCGGA
BsiEI 46 CGGCCG CG[GA][CT]^CG
Bsp1407I 3544 TGTACA T^GTACA
BspEI 1145 TCCGGA T^CCGGA
BsrDI 2593 GCAATG GCAATG(2/0)
BsrGI 3544 TGTACA T^GTACA
BssSI 1131 CTCGTG CACGAG(-5/-1)
DraIII 1990 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 46 CGGCCG C^GGCCG
Eam1105I 3567 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 46 CGGCCG C^GGCCG
Eco52I 46 CGGCCG C^GGCCG
HincII 2198 GTTAAC GT[CT]^[GA]AC
HindII 2198 GTTAAC GT[CT]^[GA]AC
HpaI 2198 GTTAAC GTT^AAC
MroI 1145 TCCGGA T^CCGGA
PshAI 478 GACGCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 662 AACGTT AA^CGTT
SapI 3819 GCTCTTC GCTCTTC(1/4)
SbfI 3300 CCTGCAGG CCTGCA^GG
SexAI 2065 ACCTGGT A^CC[AT]GGT
SfiI 20 GGCCCGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 4652 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 3300 CCTGCAGG CCTGCA^GG
SspBI 3544 TGTACA T^GTACA
XbaI 2636 TCTAGA T^CTAGA
XmnI 1600 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 2462 AAATTC [GA]^AATT[CT]
2970 GAATTT
ApoI 2462 AAATTC [GA]^AATT[CT]
2970 GAATTT
AvrII 3284 CCTAGG C^CTAGG
5080 CCTAGG
BamHI 145 GGATCC G^GATCC
901 GGATCC
BbrPI 2939 CACGTG CAC^GTG
3782 CACGTG
BcgI 1126 CGATTCTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2347 CGATTCTCCTGC
BlnI 3284 CCTAGG C^CTAGG
5080 CCTAGG
BsaWI 1145 TCCGGA [AT]^CCGG[AT]
4233 ACCGGA
BsmBI 69 GAGACG CGTCTC(1/5)
1519 GAGACG
BsmI 3381 GCATTC GAATGC(1/-1)
4508 GCATTC
BspLU11I 3547 ACATGT A^CATGT
4058 ACATGT
DrdI 1002 GACAACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5256 GACCAAGAAGTC
EcoNI 796 CCTAAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5068 CCTCTGGCAGG
MslI 4059 CATGTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4716 CACATACGTG
NaeI 30 GCCGGC GCC^GGC
3289 GCCGGC
NcoI 40 CCATGG C^CATGG
4245 CCATGG
NgoMIV 30 GCCGGC G^CCGGC
3289 GCCGGC
PmaCI 2939 CACGTG CAC^GTG
3782 CACGTG
PmlI 2939 CACGTG CAC^GTG
3782 CACGTG
PvuII 506 CAGCTG CAG^CTG
5441 CAGCTG
TaqI 1864 TCGA T^CGA
5218 TCGA
TthHB8I 1864 TCGA T^CGA
5218 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AspI AvaI AvaII AviII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I Van91I
XhoII XmaCI XmaI