################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AflII AgeI Aor51HI AscI AseI AsnI Asp718I BclI BfrI BsaBI BsiWI BspDI BspHI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII KpnI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI PvuI RcaI RsrII SalI ScaI SfuI SnaBI SpeI SphI SspI SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 3564 GTAGAC GT^[AC][GT]AC AccIII 1145 TCCGGA T^CCGGA AclI 662 AACGTT AA^CGTT AhdI 3567 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1377 GTGCAC G^TGCAC ApaLI 1377 GTGCAC G^TGCAC Asp700I 1600 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3567 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BciVI 433 GTATCC GTATCC(6/5) BglII 3485 AGATCT A^GATCT BseAI 1145 TCCGGA T^CCGGA BsiEI 46 CGGCCG CG[GA][CT]^CG Bsp1407I 3544 TGTACA T^GTACA BspEI 1145 TCCGGA T^CCGGA BsrDI 2593 GCAATG GCAATG(2/0) BsrGI 3544 TGTACA T^GTACA BssSI 1131 CTCGTG CACGAG(-5/-1) DraIII 1990 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 46 CGGCCG C^GGCCG Eam1105I 3567 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 46 CGGCCG C^GGCCG Eco52I 46 CGGCCG C^GGCCG HincII 2198 GTTAAC GT[CT]^[GA]AC HindII 2198 GTTAAC GT[CT]^[GA]AC HpaI 2198 GTTAAC GTT^AAC MroI 1145 TCCGGA T^CCGGA PshAI 478 GACGCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 662 AACGTT AA^CGTT SapI 3819 GCTCTTC GCTCTTC(1/4) SbfI 3300 CCTGCAGG CCTGCA^GG SexAI 2065 ACCTGGT A^CC[AT]GGT SfiI 20 GGCCCGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 4652 CGCCGGTG C[GA]^CCGG[CT]G Sse8387I 3300 CCTGCAGG CCTGCA^GG SspBI 3544 TGTACA T^GTACA XbaI 2636 TCTAGA T^CTAGA XmnI 1600 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 2462 AAATTC [GA]^AATT[CT] 2970 GAATTT ApoI 2462 AAATTC [GA]^AATT[CT] 2970 GAATTT AvrII 3284 CCTAGG C^CTAGG 5080 CCTAGG BamHI 145 GGATCC G^GATCC 901 GGATCC BbrPI 2939 CACGTG CAC^GTG 3782 CACGTG BcgI 1126 CGATTCTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2347 CGATTCTCCTGC BlnI 3284 CCTAGG C^CTAGG 5080 CCTAGG BsaWI 1145 TCCGGA [AT]^CCGG[AT] 4233 ACCGGA BsmBI 69 GAGACG CGTCTC(1/5) 1519 GAGACG BsmI 3381 GCATTC GAATGC(1/-1) 4508 GCATTC BspLU11I 3547 ACATGT A^CATGT 4058 ACATGT DrdI 1002 GACAACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5256 GACCAAGAAGTC EcoNI 796 CCTAAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5068 CCTCTGGCAGG MslI 4059 CATGTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4716 CACATACGTG NaeI 30 GCCGGC GCC^GGC 3289 GCCGGC NcoI 40 CCATGG C^CATGG 4245 CCATGG NgoMIV 30 GCCGGC G^CCGGC 3289 GCCGGC PmaCI 2939 CACGTG CAC^GTG 3782 CACGTG PmlI 2939 CACGTG CAC^GTG 3782 CACGTG PvuII 506 CAGCTG CAG^CTG 5441 CAGCTG TaqI 1864 TCGA T^CGA 5218 TCGA TthHB8I 1864 TCGA T^CGA 5218 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AspI AvaI AvaII AviII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XhoII XmaCI XmaI