################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII AccIII AclI AflIII AgeI Alw44I ApaLI AscI AvrII BclI BlnI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmeI Psp1406I PvuI RsrII SacI SacII SalI ScaI SfuI SgrAI SnaBI SpeI SphI SspBI SwaI XbaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AccI 1045 GTAGAC GT^[AC][GT]AC AflII 3453 CTTAAG C^TTAAG Aor51HI 1022 AGCGCT AGC^GCT AspI 3676 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2760 GGATCC G^GATCC BcgI 3042 GCACCGCGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 3453 CTTAAG C^TTAAG BglII 2922 AGATCT A^GATCT BsmBI 1173 CGTCTC CGTCTC(1/5) BsmI 5091 GCATTC GAATGC(1/-1) BspHI 3770 TCATGA T^CATGA BsrDI 4361 CATTGC GCAATG(2/0) DraI 5355 TTTAAA TTT^AAA DraIII 5237 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1022 AGCGCT AGC^GCT HpaI 5515 GTTAAC GTT^AAC PaeR7I 2649 CTCGAG C^TCGAG PflFI 3676 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 3770 TCATGA T^CATGA SapI 619 GCTCTTC GCTCTTC(1/4) SbfI 2268 CCTGCAGG CCTGCA^GG SexAI 4022 ACCAGGT A^CC[AT]GGT SfiI 2408 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 2268 CCTGCAGG CCTGCA^GG SspI 5553 AATATT AAT^ATT TfiI 4835 GAATC G^A[AT]TC Tth111I 3676 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2649 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 1973 GGTACC G^GTACC 2039 GGTACC AcsI 5133 AAATTT [GA]^AATT[CT] 5716 AAATTC AhdI 3100 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4984 GACCTGCGGTC ApoI 5133 AAATTT [GA]^AATT[CT] 5716 AAATTC AseI 3471 ATTAAT AT^TAAT 5450 ATTAAT AsnI 3471 ATTAAT AT^TAAT 5450 ATTAAT Asp700I 3993 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4964 GAACACCTTC Asp718I 1973 GGTACC G^GTACC 2039 GGTACC AspEI 3100 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4984 GACCTGCGGTC AviII 3898 TGCGCA TGC^GCA 5004 TGCGCA BciVI 485 GGATAC GTATCC(6/5) 1101 GTATCC BlpI 202 GCTGAGC GC^T[ACGT]AGC 786 GCTTAGC Bpu1102I 202 GCTGAGC GC^T[ACGT]AGC 786 GCTTAGC BsrFI 2036 GCCGGT [GA]^CCGG[CT] 2445 GCCGGT BssSI 2294 CACGAG CACGAG(-5/-1) 2993 CTCGTG CelII 202 GCTGAGC GC^T[ACGT]AGC 786 GCTTAGC Cfr10I 2036 GCCGGT [GA]^CCGG[CT] 2445 GCCGGT DrdI 1010 GACGTGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3946 GACCTAACAGTC Eam1105I 3100 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4984 GACCTGCGGTC FspI 3898 TGCGCA TGC^GCA 5004 TGCGCA HindIII 611 AAGCTT A^AGCTT 5269 AAGCTT KpnI 1973 GGTACC GGTAC^C 2039 GGTACC PshAI 245 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1995 GACGACCGTC PshBI 3471 ATTAAT AT^TAAT 5450 ATTAAT XmnI 3993 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4964 GAACACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI