##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AflIII AgeI
Alw44I ApaLI AscI AvrII BclI
BlnI BsaBI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI KspI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmeI
Psp1406I PvuI RsrII SacI SacII
SalI ScaI SfuI SgrAI SnaBI
SpeI SphI SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1045 GTAGAC GT^[AC][GT]AC
AflII 3453 CTTAAG C^TTAAG
Aor51HI 1022 AGCGCT AGC^GCT
AspI 3676 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2760 GGATCC G^GATCC
BcgI 3042 GCACCGCGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3453 CTTAAG C^TTAAG
BglII 2922 AGATCT A^GATCT
BsmBI 1173 CGTCTC CGTCTC(1/5)
BsmI 5091 GCATTC GAATGC(1/-1)
BspHI 3770 TCATGA T^CATGA
BsrDI 4361 CATTGC GCAATG(2/0)
DraI 5355 TTTAAA TTT^AAA
DraIII 5237 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1022 AGCGCT AGC^GCT
HpaI 5515 GTTAAC GTT^AAC
PaeR7I 2649 CTCGAG C^TCGAG
PflFI 3676 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 3770 TCATGA T^CATGA
SapI 619 GCTCTTC GCTCTTC(1/4)
SbfI 2268 CCTGCAGG CCTGCA^GG
SexAI 4022 ACCAGGT A^CC[AT]GGT
SfiI 2408 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2268 CCTGCAGG CCTGCA^GG
SspI 5553 AATATT AAT^ATT
TfiI 4835 GAATC G^A[AT]TC
Tth111I 3676 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2649 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 1973 GGTACC G^GTACC
2039 GGTACC
AcsI 5133 AAATTT [GA]^AATT[CT]
5716 AAATTC
AhdI 3100 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4984 GACCTGCGGTC
ApoI 5133 AAATTT [GA]^AATT[CT]
5716 AAATTC
AseI 3471 ATTAAT AT^TAAT
5450 ATTAAT
AsnI 3471 ATTAAT AT^TAAT
5450 ATTAAT
Asp700I 3993 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4964 GAACACCTTC
Asp718I 1973 GGTACC G^GTACC
2039 GGTACC
AspEI 3100 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4984 GACCTGCGGTC
AviII 3898 TGCGCA TGC^GCA
5004 TGCGCA
BciVI 485 GGATAC GTATCC(6/5)
1101 GTATCC
BlpI 202 GCTGAGC GC^T[ACGT]AGC
786 GCTTAGC
Bpu1102I 202 GCTGAGC GC^T[ACGT]AGC
786 GCTTAGC
BsrFI 2036 GCCGGT [GA]^CCGG[CT]
2445 GCCGGT
BssSI 2294 CACGAG CACGAG(-5/-1)
2993 CTCGTG
CelII 202 GCTGAGC GC^T[ACGT]AGC
786 GCTTAGC
Cfr10I 2036 GCCGGT [GA]^CCGG[CT]
2445 GCCGGT
DrdI 1010 GACGTGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3946 GACCTAACAGTC
Eam1105I 3100 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4984 GACCTGCGGTC
FspI 3898 TGCGCA TGC^GCA
5004 TGCGCA
HindIII 611 AAGCTT A^AGCTT
5269 AAGCTT
KpnI 1973 GGTACC GGTAC^C
2039 GGTACC
PshAI 245 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1995 GACGACCGTC
PshBI 3471 ATTAAT AT^TAAT
5450 ATTAAT
XmnI 3993 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4964 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI