################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AccIII AclI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AviII BcgI BlpI Bpu1102I BseAI BsiEI BsiWI BspDI BspEI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI HgaI HpaI KspI MluI MroI NaeI NgoMIV NotI NruI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI Sse8387I SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 1646 GTAGAC GT^[AC][GT]AC AccII 2143 CGCG CG^CG AflII 3837 CTTAAG C^TTAAG AspI 159 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4068 CCTAGG C^CTAGG BamHI 2271 GGATCC G^GATCC BbrPI 3455 CACGTG CAC^GTG BbsI 1132 GAAGAC GAAGAC(2/6) BfrI 3837 CTTAAG C^TTAAG BlnI 4068 CCTAGG C^CTAGG BpuAI 1132 GAAGAC GAAGAC(2/6) BsaBI 1285 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 157 GAGACG CGTCTC(1/5) BspHI 4338 TCATGA T^CATGA BsrBI 4058 CCGCTC CCGCTC(-3/-3) BssSI 2252 CACGAG CACGAG(-5/-1) BstEII 5026 GGTCACC G^GT[ACGT]ACC BstPI 5026 GGTCACC G^GT[ACGT]ACC BstUI 2143 CGCG CG^CG EcoO65I 5026 GGTCACC G^GT[ACGT]ACC MamI 1285 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 2143 CGCG CG^CG NdeI 4891 CATATG CA^TATG NheI 1063 GCTAGC G^CTAGC PflFI 159 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3455 CACGTG CAC^GTG PmlI 3455 CACGTG CAC^GTG PshAI 5063 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 4338 TCATGA T^CATGA SacI 4661 GAGCTC GAGCT^C SexAI 5013 ACCAGGT A^CC[AT]GGT SpeI 4027 ACTAGT A^CTAGT Tth111I 159 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 2202 GGTACC G^GTACC 3210 GGTACC AcyI 61 GGCGCC G[GA]^CG[CT]C 364 GGCGCC AgeI 678 ACCGGT A^CCGGT 4421 ACCGGT AlwNI 2627 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 3628 CAGGTCCTG ApaI 126 GGGCCC GGGCC^C 2314 GGGCCC Asp718I 2202 GGTACC G^GTACC 3210 GGTACC BalI 2907 TGGCCA TGG^CCA 5184 TGGCCA BbeI 61 GGCGCC GGCGC^C 364 GGCGCC BciVI 2496 GGATAC GTATCC(6/5) 3237 GTATCC BclI 1305 TGATCA T^GATCA 5324 TGATCA BglI 22 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2641 GCCTCAGCGGC BglII 1113 AGATCT A^GATCT 4502 AGATCT BsaAI 1506 CACGTA [CT]AC^GT[GA] 3455 CACGTG BsaHI 61 GGCGCC G[GA]^CG[CT]C 364 GGCGCC BsaWI 678 ACCGGT [AT]^CCGG[AT] 4421 ACCGGT BsgI 262 GTGCAG GTGCAG(16/14) 496 GTGCAG Bsp1407I 1928 TGTACA T^GTACA 5518 TGTACA BsrDI 2301 CATTGC GCAATG(2/0) 1657 GCAATG BsrFI 678 ACCGGT [GA]^CCGG[CT] 4421 ACCGGT BsrGI 1928 TGTACA T^GTACA 5518 TGTACA Cfr10I 678 ACCGGT [GA]^CCGG[CT] 4421 ACCGGT EcoRV 574 GATATC GAT^ATC 4858 GATATC FbaI 1305 TGATCA T^GATCA 5324 TGATCA Hin1I 61 GGCGCC G[GA]^CG[CT]C 364 GGCGCC KasI 61 GGCGCC G^GCGCC 364 GGCGCC KpnI 2202 GGTACC GGTAC^C 3210 GGTACC MaeII 1507 ACGT A^CGT 3456 ACGT MfeI 504 CAATTG C^AATTG 4973 CAATTG MluNI 2907 TGGCCA TGG^CCA 5184 TGGCCA MscI 2907 TGGCCA TGG^CCA 5184 TGGCCA MunI 504 CAATTG C^AATTG 4973 CAATTG NarI 61 GGCGCC GG^CGCC 364 GGCGCC NcoI 802 CCATGG C^CATGG 1652 CCATGG PinAI 678 ACCGGT A^CCGGT 4421 ACCGGT SfoI 61 GGCGCC GGC^GCC 364 GGCGCC SmaI 3091 CCCGGG CCC^GGG 3206 CCCGGG SphI 3173 GCATGC GCATG^C 3646 GCATGC SspBI 1928 TGTACA T^GTACA 5518 TGTACA XbaI 1239 TCTAGA T^CTAGA 2799 TCTAGA XmaCI 3091 CCCGGG C^CCGGG 3206 CCCGGG XmaI 3091 CCCGGG C^CCGGG 3206 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AocI ApoI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII