##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AviII BcgI BlpI
Bpu1102I BseAI BsiEI BsiWI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
FseI FspI HgaI HpaI KspI
MluI MroI NaeI NgoMIV NotI
NruI NspV PacI PaeR7I PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI Sse8387I SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1646 GTAGAC GT^[AC][GT]AC
AccII 2143 CGCG CG^CG
AflII 3837 CTTAAG C^TTAAG
AspI 159 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4068 CCTAGG C^CTAGG
BamHI 2271 GGATCC G^GATCC
BbrPI 3455 CACGTG CAC^GTG
BbsI 1132 GAAGAC GAAGAC(2/6)
BfrI 3837 CTTAAG C^TTAAG
BlnI 4068 CCTAGG C^CTAGG
BpuAI 1132 GAAGAC GAAGAC(2/6)
BsaBI 1285 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 157 GAGACG CGTCTC(1/5)
BspHI 4338 TCATGA T^CATGA
BsrBI 4058 CCGCTC CCGCTC(-3/-3)
BssSI 2252 CACGAG CACGAG(-5/-1)
BstEII 5026 GGTCACC G^GT[ACGT]ACC
BstPI 5026 GGTCACC G^GT[ACGT]ACC
BstUI 2143 CGCG CG^CG
EcoO65I 5026 GGTCACC G^GT[ACGT]ACC
MamI 1285 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2143 CGCG CG^CG
NdeI 4891 CATATG CA^TATG
NheI 1063 GCTAGC G^CTAGC
PflFI 159 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3455 CACGTG CAC^GTG
PmlI 3455 CACGTG CAC^GTG
PshAI 5063 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4338 TCATGA T^CATGA
SacI 4661 GAGCTC GAGCT^C
SexAI 5013 ACCAGGT A^CC[AT]GGT
SpeI 4027 ACTAGT A^CTAGT
Tth111I 159 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2202 GGTACC G^GTACC
3210 GGTACC
AcyI 61 GGCGCC G[GA]^CG[CT]C
364 GGCGCC
AgeI 678 ACCGGT A^CCGGT
4421 ACCGGT
AlwNI 2627 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3628 CAGGTCCTG
ApaI 126 GGGCCC GGGCC^C
2314 GGGCCC
Asp718I 2202 GGTACC G^GTACC
3210 GGTACC
BalI 2907 TGGCCA TGG^CCA
5184 TGGCCA
BbeI 61 GGCGCC GGCGC^C
364 GGCGCC
BciVI 2496 GGATAC GTATCC(6/5)
3237 GTATCC
BclI 1305 TGATCA T^GATCA
5324 TGATCA
BglI 22 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2641 GCCTCAGCGGC
BglII 1113 AGATCT A^GATCT
4502 AGATCT
BsaAI 1506 CACGTA [CT]AC^GT[GA]
3455 CACGTG
BsaHI 61 GGCGCC G[GA]^CG[CT]C
364 GGCGCC
BsaWI 678 ACCGGT [AT]^CCGG[AT]
4421 ACCGGT
BsgI 262 GTGCAG GTGCAG(16/14)
496 GTGCAG
Bsp1407I 1928 TGTACA T^GTACA
5518 TGTACA
BsrDI 2301 CATTGC GCAATG(2/0)
1657 GCAATG
BsrFI 678 ACCGGT [GA]^CCGG[CT]
4421 ACCGGT
BsrGI 1928 TGTACA T^GTACA
5518 TGTACA
Cfr10I 678 ACCGGT [GA]^CCGG[CT]
4421 ACCGGT
EcoRV 574 GATATC GAT^ATC
4858 GATATC
FbaI 1305 TGATCA T^GATCA
5324 TGATCA
Hin1I 61 GGCGCC G[GA]^CG[CT]C
364 GGCGCC
KasI 61 GGCGCC G^GCGCC
364 GGCGCC
KpnI 2202 GGTACC GGTAC^C
3210 GGTACC
MaeII 1507 ACGT A^CGT
3456 ACGT
MfeI 504 CAATTG C^AATTG
4973 CAATTG
MluNI 2907 TGGCCA TGG^CCA
5184 TGGCCA
MscI 2907 TGGCCA TGG^CCA
5184 TGGCCA
MunI 504 CAATTG C^AATTG
4973 CAATTG
NarI 61 GGCGCC GG^CGCC
364 GGCGCC
NcoI 802 CCATGG C^CATGG
1652 CCATGG
PinAI 678 ACCGGT A^CCGGT
4421 ACCGGT
SfoI 61 GGCGCC GGC^GCC
364 GGCGCC
SmaI 3091 CCCGGG CCC^GGG
3206 CCCGGG
SphI 3173 GCATGC GCATG^C
3646 GCATGC
SspBI 1928 TGTACA T^GTACA
5518 TGTACA
XbaI 1239 TCTAGA T^CTAGA
2799 TCTAGA
XmaCI 3091 CCCGGG C^CCGGG
3206 CCCGGG
XmaI 3091 CCCGGG C^CCGGG
3206 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII