##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AvaI AviII BalI BbrPI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiWI
BsoBI BspEI BssHII BssSI Bst1107I
BstZ17I CelII CpoI DrdI Eco47III
EcoNI EcoRI EcoT22I FspI KpnI
MluI MluNI MroI MscI NcoI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SgrAI SmaI
SnaBI SphI Sse8387I SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AflII 4456 CTTAAG C^TTAAG
AhdI 3615 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4461 GTGCAC G^TGCAC
AocI 4776 CCTTAGG CC^T[ACGT]AGG
ApaI 548 GGGCCC GGGCC^C
ApaLI 4461 GTGCAC G^TGCAC
AspEI 3615 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3938 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 193 CCTAGG C^CTAGG
BamHI 3977 GGATCC G^GATCC
BanI 182 GGCGCC G^G[CT][GA]CC
BbeI 182 GGCGCC GGCGC^C
BbvCI 1935 GCTGAGG CCTCAGC(-5/-2)
BcgI 321 CGAGCTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4456 CTTAAG C^TTAAG
BglI 104 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 193 CCTAGG C^CTAGG
BsaI 293 GGTCTC GGTCTC(1/5)
BsmBI 680 GAGACG CGTCTC(1/5)
BsmI 5375 GAATGC GAATGC(1/-1)
BspDI 4003 ATCGAT AT^CGAT
BspHI 376 TCATGA T^CATGA
BsrBI 202 GAGCGG CCGCTC(-3/-3)
BstAPI 4319 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3550 TTCGAA TT^CGAA
BstEII 4860 GGTAACC G^GT[ACGT]ACC
BstPI 4860 GGTAACC G^GT[ACGT]ACC
BstXI 1475 CCAAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4776 CCTTAGG CC^T[ACGT]AGG
ClaI 4003 ATCGAT AT^CGAT
DraIII 3313 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 91 CCGCGG C^C[GA][CT]GG
Eam1105I 3615 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4776 CCTTAGG CC^T[ACGT]AGG
EcoO65I 4860 GGTAACC G^GT[ACGT]ACC
EcoRV 1378 GATATC GAT^ATC
HindIII 2500 AAGCTT A^AGCTT
KasI 182 GGCGCC G^GCGCC
KspI 91 CCGCGG CCGC^GG
NarI 182 GGCGCC GG^CGCC
NspV 3550 TTCGAA TT^CGAA
PflFI 3938 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3411 CCAAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2179 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 376 TCATGA T^CATGA
SacII 91 CCGCGG CCGC^GG
SapI 2956 GCTCTTC GCTCTTC(1/4)
SexAI 339 ACCTGGT A^CC[AT]GGT
SfiI 103 GGCCCTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 182 GGCGCC GGC^GCC
SfuI 3550 TTCGAA TT^CGAA
SpeI 1077 ACTAGT A^CTAGT
Tth111I 3938 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3411 CCAAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2102 CCAAGTGCGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 273 GTAGAC GT^[AC][GT]AC
1470 GTCTAC
BclI 1826 TGATCA T^GATCA
2591 TGATCA
BglII 810 AGATCT A^GATCT
1478 AGATCT
BsrDI 1597 CATTGC GCAATG(2/0)
1622 GCAATG
EcoT14I 193 CCTAGG C^C[AT][AT]GG
359 CCTTGG
FbaI 1826 TGATCA T^GATCA
2591 TGATCA
FseI 130 GGCCGGCC GGCCGG^CC
206 GGCCGGCC
HaeII 182 GGCGCC [GA]GCGC^[CT]
2365 GGCGCT
MaeII 56 ACGT A^CGT
2840 ACGT
MfeI 1320 CAATTG C^AATTG
3580 CAATTG
MunI 1320 CAATTG C^AATTG
3580 CAATTG
SacI 3606 GAGCTC GAGCT^C
3665 GAGCTC
ScaI 1561 AGTACT AGT^ACT
5532 AGTACT
StuI 4557 AGGCCT AGG^CCT
5336 AGGCCT
StyI 193 CCTAGG C^C[AT][AT]GG
359 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BanII BbsI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspLU11I BspMI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI