##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccII AclI AflII AgeI
Aor51HI AscI AseI AsnI AviII
AvrII BamHI BciVI BclI BfrI
BlnI BsiEI BsiWI BsmBI BspDI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HpaI
KspI MluI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfuI SmaI SnaBI
SpeI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 4239 GGTACC G^GTACC
AccI 2745 GTCTAC GT^[AC][GT]AC
AccIII 2552 TCCGGA T^CCGGA
AhdI 3942 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2847 GTGCAC G^TGCAC
ApaLI 2847 GTGCAC G^TGCAC
Asp718I 4239 GGTACC G^GTACC
AspEI 3942 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2721 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5471 CTCGAG C^[CT]CG[GA]G
BbeI 2515 GGCGCC GGCGC^C
BcgI 3606 CGAGTCTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3831 AGATCT A^GATCT
BsaBI 3094 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2552 TCCGGA T^CCGGA
BsoBI 5471 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2339 TGTACA T^GTACA
BspEI 2552 TCCGGA T^CCGGA
BspHI 2147 TCATGA T^CATGA
BspLU11I 3076 ACATGT A^CATGT
BsrBI 5193 GAGCGG CCGCTC(-3/-3)
BsrGI 2339 TGTACA T^GTACA
DraI 1218 TTTAAA TTT^AAA
Eam1105I 3942 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 4819 ATGCAT ATGCA^T
HaeII 2515 GGCGCC [GA]GCGC^[CT]
KasI 2515 GGCGCC G^GCGCC
KpnI 4239 GGTACC GGTAC^C
MamI 3094 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2941 CAATTG C^AATTG
MroI 2552 TCCGGA T^CCGGA
MunI 2941 CAATTG C^AATTG
NarI 2515 GGCGCC GG^CGCC
NsiI 4819 ATGCAT ATGCA^T
PaeR7I 5471 CTCGAG C^TCGAG
PflFI 2721 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 5380 CTGCAG CTGCA^G
RcaI 2147 TCATGA T^CATGA
SfoI 2515 GGCGCC GGC^GCC
SgrAI 2295 CACCGGCG C[GA]^CCGG[CT]G
SspBI 2339 TGTACA T^GTACA
Tth111I 2721 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5471 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 4803 GGGCCC GGGCC^C
5166 GGGCCC
Asp700I 570 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4679 GAAGGCCTTC
BglI 864 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3593 GCCGAGGGGGC
BsmI 2170 GAATGC GAATGC(1/-1)
4785 GAATGC
BsrFI 2296 ACCGGC [GA]^CCGG[CT]
3453 GCCGGT
BstXI 250 CCAGGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3234 CCACAGTCTTGG
CfoI 2516 GCGC GCG^C
5333 GCGC
Cfr10I 2296 ACCGGC [GA]^CCGG[CT]
3453 GCCGGT
DrdI 695 GACTGAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3625 GACCATAGGGTC
EcoNI 3474 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4619 CCTATGTCAGG
HhaI 2516 GCGC GCG^C
5333 GCGC
HinP1I 2516 GCGC G^CGC
5333 GCGC
HincII 2160 GTTGAC GT[CT]^[GA]AC
2268 GTCAAC
HindII 2160 GTTGAC GT[CT]^[GA]AC
2268 GTCAAC
PshAI 3232 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5282 GACAGGTGTC
SexAI 607 ACCAGGT A^CC[AT]GGT
2803 ACCAGGT
SspI 2931 AATATT AAT^ATT
3348 AATATT
StuI 2432 AGGCCT AGG^CCT
4681 AGGCCT
XmnI 570 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4679 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CelII Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII