##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI AlwNI
Aor51HI AscI Asp718I AspEI AspI
AvaI AviII BalI BanI BbeI
BbrPI BbvCI BciVI BfrI BglI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmFI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CfoI ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FauI FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HpaI KasI KpnI KspI
MluI MluNI MroI MscI MslI
MspA1I MvnI NarI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 771 GACGTC GACGT^C
AcyI 771 GACGTC G[GA]^CG[CT]C
AfaI 1423 GTAC GT^AC
Alw44I 262 GTGCAC G^TGCAC
AocI 47 CCTCAGG CC^T[ACGT]AGG
ApaI 630 GGGCCC GGGCC^C
ApaLI 262 GTGCAC G^TGCAC
Asp700I 1017 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1529 GGACC G^G[AT]CC
AvrII 578 CCTAGG C^CTAGG
BamHI 269 GGATCC G^GATCC
BbsI 573 GAAGAC GAAGAC(2/6)
BcgI 1331 CGAGTGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 553 TGATCA T^GATCA
BcnI 617 CCCGG CC^[GC]GG
BglII 1278 AGATCT A^GATCT
BlnI 578 CCTAGG C^CTAGG
BlpI 687 GCTCAGC GC^T[ACGT]AGC
BpmI 534 CTCCAG CTGGAG(16/14)
Bpu1102I 687 GCTCAGC GC^T[ACGT]AGC
BpuAI 573 GAAGAC GAAGAC(2/6)
BsaAI 841 CACGTA [CT]AC^GT[GA]
BsaBI 554 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 771 GACGTC G[GA]^CG[CT]C
BsgI 203 GTGCAG GTGCAG(16/14)
BsiYI 578 CCTAGGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 578 CCTAGGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 456 GAGAC GTCTC(1/5)
BsmBI 456 GAGACG CGTCTC(1/5)
BspHI 1122 TCATGA T^CATGA
BsrFI 511 GCCGGC [GA]^CCGG[CT]
BstAPI 141 GCAGTAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1532 CCAAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 47 CCTCAGG CC^T[ACGT]AGG
CelII 687 GCTCAGC GC^T[ACGT]AGC
Cfr10I 511 GCCGGC [GA]^CCGG[CT]
DraII 629 GGGGCCC [GA]G^G[ACGT]CC[CT]
Eco81I 47 CCTCAGG CC^T[ACGT]AGG
EcoNI 578 CCTAGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 629 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 90 GATATC GAT^ATC
EcoT14I 578 CCTAGG C^C[AT][AT]GG
FbaI 553 TGATCA T^GATCA
HaeIII 631 GGCC GG^CC
Hin1I 771 GACGTC G[GA]^CG[CT]C
HindIII 246 AAGCTT A^AGCTT
MamI 554 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 284 CAATTG C^AATTG
MunI 284 CAATTG C^AATTG
NaeI 511 GCCGGC GCC^GGC
NciI 617 CCCGG CC^[GC]GG
NgoMIV 511 GCCGGC G^CCGGC
NspI 1144 GCATGT [GA]CATG^[CT]
RcaI 1122 TCATGA T^CATGA
RsaI 1423 GTAC GT^AC
SacI 685 GAGCTC GAGCT^C
SapI 858 GAAGAGC GCTCTTC(1/4)
SexAI 1220 ACCTGGT A^CC[AT]GGT
StyI 578 CCTAGG C^C[AT][AT]GG
XcmI 407 CCAAATTGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1017 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 270 GATCC GGATC(4/5)
269 GGATC
AseI 1181 ATTAAT AT^TAAT
1382 ATTAAT
AsnI 1181 ATTAAT AT^TAAT
1382 ATTAAT
AspHI 262 GTGCAC G[AT]GC[AT]^C
685 GAGCTC
BanII 630 GGGCCC G[GA]GC[CT]^C
685 GAGCTC
BsaJI 578 CCTAGG C^C[ACGT][ACGT]GG
616 CCCCGG
BsiHKAI 262 GTGCAC G[AT]GC[AT]^C
685 GAGCTC
BsmI 1021 GCATTC GAATGC(1/-1)
1549 GCATTC
BsrDI 1049 CATTGC GCAATG(2/0)
18 GCAATG
BstYI 269 GGATCC [GA]^GATC[CT]
1278 AGATCT
DraI 1085 TTTAAA TTT^AAA
1356 TTTAAA
HapII 512 CCGG C^CGG
618 CCGG
HpaII 512 CCGG C^CGG
618 CCGG
MflI 269 GGATCC [GA]^GATC[CT]
1278 AGATCT
MspI 512 CCGG C^CGG
618 CCGG
NlaIII 1123 CATG CATG^
1145 CATG
PshBI 1181 ATTAAT AT^TAAT
1382 ATTAAT
SmlI 709 CTCAAG C^T[CT][GA]AG
1407 CTCAAG
SspI 1195 AATATT AAT^ATT
1231 AATATT
TaqI 1330 TCGA T^CGA
1491 TCGA
TthHB8I 1330 TCGA T^CGA
1491 TCGA
XhoII 269 GGATCC [GA]^GATC[CT]
1278 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BfaI BmyI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT22I Fnu4HI
FokI HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIV NsiI Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI