################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI AlwNI Aor51HI AscI Asp718I AspEI AspI AvaI AviII BalI BanI BbeI BbrPI BbvCI BciVI BfrI BglI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CfoI ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI FauI FseI FspI HaeII HgaI HhaI HinP1I HincII HindII HpaI KasI KpnI KspI MluI MluNI MroI MscI MslI MspA1I MvnI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 771 GACGTC GACGT^C AcyI 771 GACGTC G[GA]^CG[CT]C AfaI 1423 GTAC GT^AC Alw44I 262 GTGCAC G^TGCAC AocI 47 CCTCAGG CC^T[ACGT]AGG ApaI 630 GGGCCC GGGCC^C ApaLI 262 GTGCAC G^TGCAC Asp700I 1017 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 1529 GGACC G^G[AT]CC AvrII 578 CCTAGG C^CTAGG BamHI 269 GGATCC G^GATCC BbsI 573 GAAGAC GAAGAC(2/6) BcgI 1331 CGAGTGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 553 TGATCA T^GATCA BcnI 617 CCCGG CC^[GC]GG BglII 1278 AGATCT A^GATCT BlnI 578 CCTAGG C^CTAGG BlpI 687 GCTCAGC GC^T[ACGT]AGC BpmI 534 CTCCAG CTGGAG(16/14) Bpu1102I 687 GCTCAGC GC^T[ACGT]AGC BpuAI 573 GAAGAC GAAGAC(2/6) BsaAI 841 CACGTA [CT]AC^GT[GA] BsaBI 554 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 771 GACGTC G[GA]^CG[CT]C BsgI 203 GTGCAG GTGCAG(16/14) BsiYI 578 CCTAGGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 578 CCTAGGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 456 GAGAC GTCTC(1/5) BsmBI 456 GAGACG CGTCTC(1/5) BspHI 1122 TCATGA T^CATGA BsrFI 511 GCCGGC [GA]^CCGG[CT] BstAPI 141 GCAGTAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1532 CCAAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 47 CCTCAGG CC^T[ACGT]AGG CelII 687 GCTCAGC GC^T[ACGT]AGC Cfr10I 511 GCCGGC [GA]^CCGG[CT] DraII 629 GGGGCCC [GA]G^G[ACGT]CC[CT] Eco81I 47 CCTCAGG CC^T[ACGT]AGG EcoNI 578 CCTAGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 629 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoRV 90 GATATC GAT^ATC EcoT14I 578 CCTAGG C^C[AT][AT]GG FbaI 553 TGATCA T^GATCA HaeIII 631 GGCC GG^CC Hin1I 771 GACGTC G[GA]^CG[CT]C HindIII 246 AAGCTT A^AGCTT MamI 554 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 284 CAATTG C^AATTG MunI 284 CAATTG C^AATTG NaeI 511 GCCGGC GCC^GGC NciI 617 CCCGG CC^[GC]GG NgoMIV 511 GCCGGC G^CCGGC NspI 1144 GCATGT [GA]CATG^[CT] RcaI 1122 TCATGA T^CATGA RsaI 1423 GTAC GT^AC SacI 685 GAGCTC GAGCT^C SapI 858 GAAGAGC GCTCTTC(1/4) SexAI 1220 ACCTGGT A^CC[AT]GGT StyI 578 CCTAGG C^C[AT][AT]GG XcmI 407 CCAAATTGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1017 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 270 GATCC GGATC(4/5) 269 GGATC AseI 1181 ATTAAT AT^TAAT 1382 ATTAAT AsnI 1181 ATTAAT AT^TAAT 1382 ATTAAT AspHI 262 GTGCAC G[AT]GC[AT]^C 685 GAGCTC BanII 630 GGGCCC G[GA]GC[CT]^C 685 GAGCTC BsaJI 578 CCTAGG C^C[ACGT][ACGT]GG 616 CCCCGG BsiHKAI 262 GTGCAC G[AT]GC[AT]^C 685 GAGCTC BsmI 1021 GCATTC GAATGC(1/-1) 1549 GCATTC BsrDI 1049 CATTGC GCAATG(2/0) 18 GCAATG BstYI 269 GGATCC [GA]^GATC[CT] 1278 AGATCT DraI 1085 TTTAAA TTT^AAA 1356 TTTAAA HapII 512 CCGG C^CGG 618 CCGG HpaII 512 CCGG C^CGG 618 CCGG MflI 269 GGATCC [GA]^GATC[CT] 1278 AGATCT MspI 512 CCGG C^CGG 618 CCGG NlaIII 1123 CATG CATG^ 1145 CATG PshBI 1181 ATTAAT AT^TAAT 1382 ATTAAT SmlI 709 CTCAAG C^T[CT][GA]AG 1407 CTCAAG SspI 1195 AATATT AAT^ATT 1231 AATATT TaqI 1330 TCGA T^CGA 1491 TCGA TthHB8I 1330 TCGA T^CGA 1491 TCGA XhoII 269 GGATCC [GA]^GATC[CT] 1278 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AcsI AluI ApoI BbvI BfaI BmyI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT22I Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIV NsiI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI