##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Aor51HI AscI AseI
AsnI Asp718I AviII BbeI BbrPI
BciVI BclI BfrI BsaAI BseAI
BsiWI Bsp1407I BspDI BspEI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI Eco47III EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SfoI SfuI SnaBI
SpeI SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 5394 ACATGT A^C[GA][CT]GT
AhdI 2055 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4116 CCTGAGG CC^T[ACGT]AGG
AspEI 2055 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglII 2578 AGATCT A^GATCT
BsaBI 422 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1425 CGGCCG CG[GA][CT]^CG
BsmBI 5761 CGTCTC CGTCTC(1/5)
BspHI 5322 TCATGA T^CATGA
BspLU11I 5394 ACATGT A^CATGT
BsrBI 385 GAGCGG CCGCTC(-3/-3)
BsrFI 3415 ACCGGC [GA]^CCGG[CT]
Bsu36I 4116 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3415 ACCGGC [GA]^CCGG[CT]
DraI 4918 TTTAAA TTT^AAA
DrdI 4574 GACTCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1425 CGGCCG C^GGCCG
Eam1105I 2055 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1425 CGGCCG C^GGCCG
Eco52I 1425 CGGCCG C^GGCCG
Eco81I 4116 CCTGAGG CC^T[ACGT]AGG
MamI 422 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4869 CATATG CA^TATG
PaeR7I 3407 CTCGAG C^TCGAG
PshAI 2226 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5322 TCATGA T^CATGA
SbfI 3516 CCTGCAGG CCTGCA^GG
SexAI 797 ACCTGGT A^CC[AT]GGT
SfiI 3756 GGCCACTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 3414 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 3516 CCTGCAGG CCTGCA^GG
XhoI 3407 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 1298 GGCGTC G[GA]^CG[CT]C
2068 GACGCC
Alw44I 3288 GTGCAC G^TGCAC
5802 GTGCAC
ApaLI 3288 GTGCAC G^TGCAC
5802 GTGCAC
Asp700I 1834 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4424 GAAGACATTC
AvrII 2551 CCTAGG C^CTAGG
3875 CCTAGG
BbsI 4424 GAAGAC GAAGAC(2/6)
1786 GTCTTC
BcgI 3106 CGACACTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4991 GCAGGTAGATCG
BlnI 2551 CCTAGG C^CTAGG
3875 CCTAGG
BpuAI 4424 GAAGAC GAAGAC(2/6)
1786 GTCTTC
BsaHI 1298 GGCGTC G[GA]^CG[CT]C
2068 GACGCC
BsaWI 509 ACCGGA [AT]^CCGG[AT]
1598 ACCGGA
Hin1I 1298 GGCGTC G[GA]^CG[CT]C
2068 GACGCC
MfeI 4801 CAATTG C^AATTG
5909 CAATTG
MunI 4801 CAATTG C^AATTG
5909 CAATTG
SacI 339 GAGCTC GAGCT^C
1864 GAGCTC
SapI 1491 GAAGAGC GCTCTTC(1/4)
2216 GCTCTTC
ScaI 2254 AGTACT AGT^ACT
4787 AGTACT
SphI 28 GCATGC GCATG^C
991 GCATGC
XbaI 4713 TCTAGA T^CTAGA
4819 TCTAGA
XmnI 1834 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4424 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI