##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI AspEI AvrII BbrPI
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
Eam1105I Eco47III EcoO65I FseI HpaI
MamI MluI MroI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2973 GGTACC G^GTACC
AflII 4838 CTTAAG C^TTAAG
AflIII 1275 ACATGT A^C[GA][CT]GT
Alw44I 1279 GTGCAC G^TGCAC
ApaLI 1279 GTGCAC G^TGCAC
AseI 2808 ATTAAT AT^TAAT
AsnI 2808 ATTAAT AT^TAAT
Asp700I 3617 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2973 GGTACC G^GTACC
AviII 1354 TGCGCA TGC^GCA
BalI 5523 TGGCCA TGG^CCA
BbvCI 193 GCTGAGG CCTCAGC(-5/-2)
BcgI 5140 GCACCAAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3502 TGATCA T^GATCA
BfrI 4838 CTTAAG C^TTAAG
BlpI 1821 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1821 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 938 TGTACA T^GTACA
BspHI 1300 TCATGA T^CATGA
BspLU11I 1275 ACATGT A^CATGT
BsrGI 938 TGTACA T^GTACA
BssSI 4663 CACGAG CACGAG(-5/-1)
CelII 1821 GCTGAGC GC^T[ACGT]AGC
DrdI 5241 GACAAAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2106 GAATTC G^AATTC
EcoRV 432 GATATC GAT^ATC
FbaI 3502 TGATCA T^GATCA
FspI 1354 TGCGCA TGC^GCA
KpnI 2973 GGTACC GGTAC^C
KspI 3434 CCGCGG CCGC^GG
MfeI 4828 CAATTG C^AATTG
MluNI 5523 TGGCCA TGG^CCA
MscI 5523 TGGCCA TGG^CCA
MunI 4828 CAATTG C^AATTG
PaeR7I 3352 CTCGAG C^TCGAG
PshAI 5600 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2808 ATTAAT AT^TAAT
RcaI 1300 TCATGA T^CATGA
SacI 2865 GAGCTC GAGCT^C
SacII 3434 CCGCGG CCGC^GG
SphI 539 GCATGC GCATG^C
SspBI 938 TGTACA T^GTACA
SspI 5713 AATATT AAT^ATT
XbaI 5871 TCTAGA T^CTAGA
XhoI 3352 CTCGAG C^TCGAG
XmnI 3617 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 351 GTAGAC GT^[AC][GT]AC
786 GTAGAC
AcsI 2106 GAATTC [GA]^AATT[CT]
5218 AAATTT
ApoI 2106 GAATTC [GA]^AATT[CT]
5218 AAATTT
AspI 3057 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3675 GACAAAGTC
BamHI 1108 GGATCC G^GATCC
2048 GGATCC
BbeI 1053 GGCGCC GGCGC^C
4871 GGCGCC
BciVI 4254 GGATAC GTATCC(6/5)
2613 GTATCC
BglII 1681 AGATCT A^GATCT
5625 AGATCT
BsiEI 32 CGGCCG CG[GA][CT]^CG
2968 CGGCCG
BspDI 849 ATCGAT AT^CGAT
3507 ATCGAT
BsrDI 4351 CATTGC GCAATG(2/0)
901 GCAATG
ClaI 849 ATCGAT AT^CGAT
3507 ATCGAT
EagI 32 CGGCCG C^GGCCG
2968 CGGCCG
EclXI 32 CGGCCG C^GGCCG
2968 CGGCCG
Eco52I 32 CGGCCG C^GGCCG
2968 CGGCCG
EcoT22I 541 ATGCAT ATGCA^T
4956 ATGCAT
HincII 2076 GTCAAC GT[CT]^[GA]AC
4587 GTTGAC
HindII 2076 GTCAAC GT[CT]^[GA]AC
4587 GTTGAC
HindIII 3298 AAGCTT A^AGCTT
4841 AAGCTT
KasI 1053 GGCGCC G^GCGCC
4871 GGCGCC
MaeII 2401 ACGT A^CGT
3574 ACGT
MslI 4728 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4857 CACCTGGATG
NaeI 265 GCCGGC GCC^GGC
3926 GCCGGC
NarI 1053 GGCGCC GG^CGCC
4871 GGCGCC
NcoI 1981 CCATGG C^CATGG
3083 CCATGG
NdeI 322 CATATG CA^TATG
5428 CATATG
NgoMIV 265 GCCGGC G^CCGGC
3926 GCCGGC
NsiI 541 ATGCAT ATGCA^T
4956 ATGCAT
NspI 539 GCATGC [GA]CATG^[CT]
1275 ACATGT
PflFI 3057 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3675 GACAAAGTC
SexAI 1388 ACCAGGT A^CC[AT]GGT
2482 ACCTGGT
SfoI 1053 GGCGCC GGC^GCC
4871 GGCGCC
Tth111I 3057 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3675 GACAAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI