##################################
# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BspMI BsrBI BsrDI BsrFI BssHII
BstBI BstEII BstPI BstUI BstXI
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaI HpaII KasI KpnI
KspI MaeII MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflIII 82 ACATGT A^C[GA][CT]GT
AlwNI 1525 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 934 ATTAAT AT^TAAT
AsnI 934 ATTAAT AT^TAAT
BamHI 477 GGATCC G^GATCC
BanII 1540 GAGCTC G[GA]GC[CT]^C
BfaI 1252 CTAG C^TAG
BglII 1401 AGATCT A^GATCT
BpmI 392 CTCCAG CTGGAG(16/14)
BsaI 1281 GGTCTC GGTCTC(1/5)
BseRI 483 CTCCTC GAGGAG(10/8)
BsgI 161 GTGCAG GTGCAG(16/14)
BsmI 452 GCATTC GAATGC(1/-1)
Bsp1407I 1684 TGTACA T^GTACA
BspHI 923 TCATGA T^CATGA
BspLU11I 82 ACATGT A^CATGT
BsrGI 1684 TGTACA T^GTACA
BssSI 565 CACGAG CACGAG(-5/-1)
Bst1107I 1224 GTATAC GTA^TAC
BstAPI 1045 GCAAACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1224 GTATAC GTA^TAC
EcoNI 485 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 596 GATATC GAT^ATC
EcoT14I 95 CCTTGG C^C[AT][AT]GG
HincII 776 GTCAAC GT[CT]^[GA]AC
HindII 776 GTCAAC GT[CT]^[GA]AC
HindIII 1015 AAGCTT A^AGCTT
MaeI 1252 CTAG C^TAG
MslI 127 CATAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1492 CAGCTG C[AC]G^C[GT]G
MwoI 1045 GCAAACCGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 82 ACATGT [GA]CATG^[CT]
PshBI 934 ATTAAT AT^TAAT
PstI 751 CTGCAG CTGCA^G
PvuII 1492 CAGCTG CAG^CTG
RcaI 923 TCATGA T^CATGA
SacI 1540 GAGCTC GAGCT^C
SapI 551 GAAGAGC GCTCTTC(1/4)
ScaI 1479 AGTACT AGT^ACT
SspBI 1684 TGTACA T^GTACA
StyI 95 CCTTGG C^C[AT][AT]GG
TaqI 1194 TCGA T^CGA
Tsp45I 1573 GTGAC ^GT[GC]AC
TthHB8I 1194 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BsaBI 844 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1231 GATTACTATC
BsaJI 95 CCTTGG C^C[ACGT][ACGT]GG
1214 CCCTGG
BsmAI 948 GTCTC GTCTC(1/5)
1282 GTCTC
BstYI 477 GGATCC [GA]^GATC[CT]
1401 AGATCT
Cfr13I 23 GGCCC G^G[ACGT]CC
685 GGCCC
DraI 1033 TTTAAA TTT^AAA
1204 TTTAAA
EarI 551 GAAGAG CTCTTC(1/4)
721 GAAGAG
HaeIII 23 GGCC GG^CC
685 GGCC
Ksp632I 551 GAAGAG CTCTTC(1/4)
721 GAAGAG
MaeIII 1564 GTTAC ^GT[ACGT]AC
1573 GTGAC
MamI 844 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1231 GATTACTATC
MflI 477 GGATCC [GA]^GATC[CT]
1401 AGATCT
NdeI 75 CATATG CA^TATG
458 CATATG
PleI 946 GAGTC GAGTC(4/5)
1115 GAGTC
Sau96I 23 GGCCC G^G[ACGT]CC
685 GGCCC
XhoII 477 GGATCC [GA]^GATC[CT]
1401 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI BbvI BmyI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRI EcoRII Fnu4HI FokI HinfI
HphI ItaI MboI MboII MnlI
MseI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI
XmnI