################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspMI BsrBI BsrDI BsrFI BssHII BstBI BstEII BstPI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MaeII MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AflIII 82 ACATGT A^C[GA][CT]GT AlwNI 1525 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 934 ATTAAT AT^TAAT AsnI 934 ATTAAT AT^TAAT BamHI 477 GGATCC G^GATCC BanII 1540 GAGCTC G[GA]GC[CT]^C BfaI 1252 CTAG C^TAG BglII 1401 AGATCT A^GATCT BpmI 392 CTCCAG CTGGAG(16/14) BsaI 1281 GGTCTC GGTCTC(1/5) BseRI 483 CTCCTC GAGGAG(10/8) BsgI 161 GTGCAG GTGCAG(16/14) BsmI 452 GCATTC GAATGC(1/-1) Bsp1407I 1684 TGTACA T^GTACA BspHI 923 TCATGA T^CATGA BspLU11I 82 ACATGT A^CATGT BsrGI 1684 TGTACA T^GTACA BssSI 565 CACGAG CACGAG(-5/-1) Bst1107I 1224 GTATAC GTA^TAC BstAPI 1045 GCAAACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 1224 GTATAC GTA^TAC EcoNI 485 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 596 GATATC GAT^ATC EcoT14I 95 CCTTGG C^C[AT][AT]GG HincII 776 GTCAAC GT[CT]^[GA]AC HindII 776 GTCAAC GT[CT]^[GA]AC HindIII 1015 AAGCTT A^AGCTT MaeI 1252 CTAG C^TAG MslI 127 CATAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1492 CAGCTG C[AC]G^C[GT]G MwoI 1045 GCAAACCGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 82 ACATGT [GA]CATG^[CT] PshBI 934 ATTAAT AT^TAAT PstI 751 CTGCAG CTGCA^G PvuII 1492 CAGCTG CAG^CTG RcaI 923 TCATGA T^CATGA SacI 1540 GAGCTC GAGCT^C SapI 551 GAAGAGC GCTCTTC(1/4) ScaI 1479 AGTACT AGT^ACT SspBI 1684 TGTACA T^GTACA StyI 95 CCTTGG C^C[AT][AT]GG TaqI 1194 TCGA T^CGA Tsp45I 1573 GTGAC ^GT[GC]AC TthHB8I 1194 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BsaBI 844 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1231 GATTACTATC BsaJI 95 CCTTGG C^C[ACGT][ACGT]GG 1214 CCCTGG BsmAI 948 GTCTC GTCTC(1/5) 1282 GTCTC BstYI 477 GGATCC [GA]^GATC[CT] 1401 AGATCT Cfr13I 23 GGCCC G^G[ACGT]CC 685 GGCCC DraI 1033 TTTAAA TTT^AAA 1204 TTTAAA EarI 551 GAAGAG CTCTTC(1/4) 721 GAAGAG HaeIII 23 GGCC GG^CC 685 GGCC Ksp632I 551 GAAGAG CTCTTC(1/4) 721 GAAGAG MaeIII 1564 GTTAC ^GT[ACGT]AC 1573 GTGAC MamI 844 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1231 GATTACTATC MflI 477 GGATCC [GA]^GATC[CT] 1401 AGATCT NdeI 75 CATATG CA^TATG 458 CATATG PleI 946 GAGTC GAGTC(4/5) 1115 GAGTC Sau96I 23 GGCCC G^G[ACGT]CC 685 GGCCC XhoII 477 GGATCC [GA]^GATC[CT] 1401 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AccI AcsI AfaI AluI AlwI ApoI Asp700I AspHI BbvI BmyI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI DdeI DpnII EcoRI EcoRII Fnu4HI FokI HinfI HphI ItaI MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI XmnI