##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AvrII BbeI BbrPI BbvCI
BcgI BglI BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspMI BsrBI BssHII
ClaI CpoI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI FauI
FseI Hin1I KasI KpnI KspI
MamI MluI MroI NaeI NarI
NcoI NgoMIV NheI NotI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SnaBI SpeI SphI
Sse8387I Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 2707 AACGTT AA^CGTT
AflII 4519 CTTAAG C^TTAAG
AspI 5336 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1732 TGCGCA TGC^GCA
BamHI 1779 GGATCC G^GATCC
BfrI 4519 CTTAAG C^TTAAG
BlpI 1743 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1743 GCTGAGC GC^T[ACGT]AGC
BsaI 1813 GGTCTC GGTCTC(1/5)
BsgI 3166 CTGCAC GTGCAG(16/14)
BsiEI 38 CGACCG CG[GA][CT]^CG
BspHI 1622 TCATGA T^CATGA
BsrFI 1960 ACCGGC [GA]^CCGG[CT]
BssSI 967 CACGAG CACGAG(-5/-1)
BstEII 1433 GGTTACC G^GT[ACGT]ACC
BstPI 1433 GGTTACC G^GT[ACGT]ACC
CelII 1743 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1960 ACCGGC [GA]^CCGG[CT]
DraII 177 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 2202 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2357 GACTAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 177 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1433 GGTTACC G^GT[ACGT]ACC
EcoRI 973 GAATTC G^AATTC
FspI 1732 TGCGCA TGC^GCA
HaeII 3053 AGCGCC [GA]GCGC^[CT]
HgaI 24 GCGTC GACGC(5/10)
HindIII 4655 AAGCTT A^AGCTT
HpaI 206 GTTAAC GTT^AAC
MfeI 5679 CAATTG C^AATTG
MunI 5679 CAATTG C^AATTG
NruI 31 TCGCGA TCG^CGA
PflFI 5336 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 177 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2707 AACGTT AA^CGTT
PstI 4027 CTGCAG CTGCA^G
RcaI 1622 TCATGA T^CATGA
SgrAI 1959 CACCGGCG C[GA]^CCGG[CT]G
StuI 1066 AGGCCT AGG^CCT
Tth111I 5336 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 23 CGCG CG^CG
32 CGCG
Alw44I 842 GTGCAC G^TGCAC
5256 GTGCAC
ApaLI 842 GTGCAC G^TGCAC
5256 GTGCAC
AvaII 178 GGACC G^G[AT]CC
276 GGACC
BanII 4862 GAGCCC G[GA]GC[CT]^C
5127 GAGCCC
BciVI 1425 GGATAC GTATCC(6/5)
4544 GTATCC
BclI 752 TGATCA T^GATCA
1919 TGATCA
BsmFI 4530 GGGAC GGGAC(10/14)
1317 GTCCC
BstBI 2776 TTCGAA TT^CGAA
5515 TTCGAA
BstUI 23 CGCG CG^CG
32 CGCG
BstXI 4486 CCAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4549 CCAGTTATTTGG
EcoRV 1179 GATATC GAT^ATC
4581 GATATC
FbaI 752 TGATCA T^GATCA
1919 TGATCA
MaeII 2708 ACGT A^CGT
5030 ACGT
MspA1I 1168 CAGCTG C[AC]G^C[GT]G
1522 CAGCTG
MvnI 23 CGCG CG^CG
32 CGCG
NspV 2776 TTCGAA TT^CGAA
5515 TTCGAA
PvuII 1168 CAGCTG CAG^CTG
1522 CAGCTG
SapI 6138 GAAGAGC GCTCTTC(1/4)
5 GCTCTTC
SfuI 2776 TTCGAA TT^CGAA
5515 TTCGAA
SmaI 82 CCCGGG CCC^GGG
190 CCCGGG
XbaI 1160 TCTAGA T^CTAGA
4007 TCTAGA
XmaCI 82 CCCGGG C^CCGGG
190 CCCGGG
XmaI 82 CCCGGG C^CCGGG
190 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspHI AvaI
BalI BanI BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmI BsoBI Bsp1286I Bsp1407I
BspLU11I BsrDI BsrGI BsrI BssKI
Bst1107I BstAPI BstF5I BstNI BstYI
BstZ17I Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
Eco81I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NsiI NspI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI SwaI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI