##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
AspEI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BlnI BsaAI
BsaHI BsiWI BsmBI BspDI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRV
FseI FspI Hin1I HpaI KasI
KspI MfeI MluI MunI NarI
NdeI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 4366 GTCTAC GT^[AC][GT]AC
AflII 4475 CTTAAG C^TTAAG
AflIII 3079 ACATGT A^C[GA][CT]GT
AocI 3321 CCTAAGG CC^T[ACGT]AGG
Aor51HI 4868 AGCGCT AGC^GCT
AscI 3822 GGCGCGCC GG^CGCGCC
AseI 4748 ATTAAT AT^TAAT
AsnI 4748 ATTAAT AT^TAAT
AspI 4606 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 1048 TGATCA T^GATCA
BfrI 4475 CTTAAG C^TTAAG
BsaBI 1070 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 184 CGGTCG CG[GA][CT]^CG
Bsp1407I 2271 TGTACA T^GTACA
BspLU11I 3079 ACATGT A^CATGT
BsrBI 2826 CCGCTC CCGCTC(-3/-3)
BsrGI 2271 TGTACA T^GTACA
BssHII 3823 GCGCGC G^CGCGC
Bsu36I 3321 CCTAAGG CC^T[ACGT]AGG
DrdI 215 GACGCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4868 AGCGCT AGC^GCT
Eco81I 3321 CCTAAGG CC^T[ACGT]AGG
FbaI 1048 TGATCA T^GATCA
HincII 3001 GTTGAC GT[CT]^[GA]AC
HindII 3001 GTTGAC GT[CT]^[GA]AC
HindIII 4710 AAGCTT A^AGCTT
MamI 1070 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 2698 CTCGAG C^TCGAG
PflFI 4606 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 822 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4748 ATTAAT AT^TAAT
SbfI 5045 CCTGCAGG CCTGCA^GG
ScaI 3290 AGTACT AGT^ACT
SphI 4349 GCATGC GCATG^C
Sse8387I 5045 CCTGCAGG CCTGCA^GG
SspBI 2271 TGTACA T^GTACA
Tth111I 4606 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2698 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 396 GGTACC G^GTACC
808 GGTACC
AccIII 1066 TCCGGA T^CCGGA
3921 TCCGGA
Asp700I 2602 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5532 GAAGATGTTC
Asp718I 396 GGTACC G^GTACC
808 GGTACC
BbvCI 126 GCTGAGG CCTCAGC(-5/-2)
2213 GCTGAGG
BsaI 820 GAGACC GGTCTC(1/5)
4049 GGTCTC
BseAI 1066 TCCGGA T^CCGGA
3921 TCCGGA
BspEI 1066 TCCGGA T^CCGGA
3921 TCCGGA
BspHI 1304 TCATGA T^CATGA
1666 TCATGA
BsrDI 794 CATTGC GCAATG(2/0)
1865 CATTGC
BssSI 865 CTCGTG CACGAG(-5/-1)
3468 CTCGTG
BstEII 1139 GGTCACC G^GT[ACGT]ACC
1337 GGTCACC
BstPI 1139 GGTCACC G^GT[ACGT]ACC
1337 GGTCACC
BstXI 410 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4488 CCACCTTCCTGG
DraI 4622 TTTAAA TTT^AAA
5585 TTTAAA
EcoO65I 1139 GGTCACC G^GT[ACGT]ACC
1337 GGTCACC
EcoT22I 4092 ATGCAT ATGCA^T
4347 ATGCAT
KpnI 396 GGTACC GGTAC^C
808 GGTACC
MroI 1066 TCCGGA T^CCGGA
3921 TCCGGA
NaeI 29 GCCGGC GCC^GGC
1981 GCCGGC
NgoMIV 29 GCCGGC G^CCGGC
1981 GCCGGC
NsiI 4092 ATGCAT ATGCA^T
4347 ATGCAT
RcaI 1304 TCATGA T^CATGA
1666 TCATGA
SacI 2693 GAGCTC GAGCT^C
5170 GAGCTC
SapI 5167 GAAGAGC GCTCTTC(1/4)
2986 GCTCTTC
SfiI 78 GGCCGCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
197 GGCCCTGCTGGCC
StuI 44 AGGCCT AGG^CCT
2666 AGGCCT
XmnI 2602 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5532 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII