##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BamHI BbeI BcgI
BfrI BlnI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BsrFI BssHII BstBI BstEII
BstPI BstUI BstXI Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI Hin1I HpaI KasI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflMI PinAI PmeI PshAI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I StuI
Van91I
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 993 GTATAC GT^[AC][GT]AC
AlwI 366 GGATC GGATC(4/5)
AocI 2786 CCTCAGG CC^T[ACGT]AGG
BbrPI 403 CACGTG CAC^GTG
BciVI 4150 GTATCC GTATCC(6/5)
BglI 1300 GCCTTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 295 TCCGGT [AT]^CCGG[AT]
BsmI 2682 GCATTC GAATGC(1/-1)
Bsp1407I 4419 TGTACA T^GTACA
BspHI 3770 TCATGA T^CATGA
BsrGI 4419 TGTACA T^GTACA
BssSI 396 CACGAG CACGAG(-5/-1)
Bst1107I 993 GTATAC GTA^TAC
BstZ17I 993 GTATAC GTA^TAC
Bsu36I 2786 CCTCAGG CC^T[ACGT]AGG
DraIII 3949 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2786 CCTCAGG CC^T[ACGT]AGG
FauI 644 CCCGC CCCGC(4/6)
HaeII 16 GGCGCT [GA]GCGC^[CT]
NcoI 3105 CCATGG C^CATGG
NheI 426 GCTAGC G^CTAGC
PaeR7I 2722 CTCGAG C^TCGAG
PmaCI 403 CACGTG CAC^GTG
PmlI 403 CACGTG CAC^GTG
PstI 2080 CTGCAG CTGCA^G
RcaI 3770 TCATGA T^CATGA
SpeI 2587 ACTAGT A^CTAGT
SspBI 4419 TGTACA T^GTACA
SwaI 4099 ATTTAAAT ATTT^AAAT
XhoI 2722 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 298 GGTACC G^GTACC
4513 GGTACC
AclI 2410 AACGTT AA^CGTT
4265 AACGTT
Asp700I 4367 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4549 GAATTGTTTC
Asp718I 298 GGTACC G^GTACC
4513 GGTACC
AspI 156 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2446 GACGAAGTC
BalI 386 TGGCCA TGG^CCA
4593 TGGCCA
BanI 298 GGTACC G^G[CT][GA]CC
4513 GGTACC
BbvCI 198 CCTCAGC CCTCAGC(-5/-2)
2422 GCTGAGG
BclI 2976 TGATCA T^GATCA
4005 TGATCA
BglII 290 AGATCT A^GATCT
2482 AGATCT
BlpI 799 GCTGAGC GC^T[ACGT]AGC
2775 GCTCAGC
Bpu1102I 799 GCTGAGC GC^T[ACGT]AGC
2775 GCTCAGC
BsaAI 403 CACGTG [CT]AC^GT[GA]
749 CACGTA
BsgI 3836 CTGCAC GTGCAG(16/14)
3271 GTGCAG
BsmBI 619 GAGACG CGTCTC(1/5)
1174 GAGACG
BspMI 3777 GCAGGT ACCTGC(4/8)
4146 GCAGGT
BstAPI 1180 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3448 GCACCAGATGC
CelII 799 GCTGAGC GC^T[ACGT]AGC
2775 GCTCAGC
DsaI 1001 CCACGG C^C[GA][CT]GG
3105 CCATGG
EaeI 386 TGGCCA [CT]^GGCC[GA]
4593 TGGCCA
EcoNI 309 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2355 CCTAAAGCAGG
FbaI 2976 TGATCA T^GATCA
4005 TGATCA
HincII 47 GTCAAC GT[CT]^[GA]AC
3310 GTCAAC
HindII 47 GTCAAC GT[CT]^[GA]AC
3310 GTCAAC
KpnI 298 GGTACC GGTAC^C
4513 GGTACC
MluNI 386 TGGCCA TGG^CCA
4593 TGGCCA
MscI 386 TGGCCA TGG^CCA
4593 TGGCCA
PflFI 156 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2446 GACGAAGTC
Psp1406I 2410 AACGTT AA^CGTT
4265 AACGTT
SapI 1621 GAAGAGC GCTCTTC(1/4)
2278 GAAGAGC
SmaI 437 CCCGGG CCC^GGG
956 CCCGGG
SphI 639 GCATGC GCATG^C
1050 GCATGC
Tth111I 156 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2446 GACGAAGTC
XbaI 2700 TCTAGA T^CTAGA
4375 TCTAGA
XmaCI 437 CCCGGG C^CCGGG
956 CCCGGG
XmaI 437 CCCGGG C^CCGGG
956 CCCGGG
XmnI 4367 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4549 GAATTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DrdI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII