################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII AccII AccIII AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AviII AvrII BamHI BbeI BcgI BfrI BlnI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BspDI BspEI BsrBI BsrFI BssHII BstBI BstEII BstPI BstUI BstXI Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PflMI PinAI PmeI PshAI PshBI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I StuI Van91I ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccI 993 GTATAC GT^[AC][GT]AC AlwI 366 GGATC GGATC(4/5) AocI 2786 CCTCAGG CC^T[ACGT]AGG BbrPI 403 CACGTG CAC^GTG BciVI 4150 GTATCC GTATCC(6/5) BglI 1300 GCCTTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 295 TCCGGT [AT]^CCGG[AT] BsmI 2682 GCATTC GAATGC(1/-1) Bsp1407I 4419 TGTACA T^GTACA BspHI 3770 TCATGA T^CATGA BsrGI 4419 TGTACA T^GTACA BssSI 396 CACGAG CACGAG(-5/-1) Bst1107I 993 GTATAC GTA^TAC BstZ17I 993 GTATAC GTA^TAC Bsu36I 2786 CCTCAGG CC^T[ACGT]AGG DraIII 3949 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 2786 CCTCAGG CC^T[ACGT]AGG FauI 644 CCCGC CCCGC(4/6) HaeII 16 GGCGCT [GA]GCGC^[CT] NcoI 3105 CCATGG C^CATGG NheI 426 GCTAGC G^CTAGC PaeR7I 2722 CTCGAG C^TCGAG PmaCI 403 CACGTG CAC^GTG PmlI 403 CACGTG CAC^GTG PstI 2080 CTGCAG CTGCA^G RcaI 3770 TCATGA T^CATGA SpeI 2587 ACTAGT A^CTAGT SspBI 4419 TGTACA T^GTACA SwaI 4099 ATTTAAAT ATTT^AAAT XhoI 2722 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 298 GGTACC G^GTACC 4513 GGTACC AclI 2410 AACGTT AA^CGTT 4265 AACGTT Asp700I 4367 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4549 GAATTGTTTC Asp718I 298 GGTACC G^GTACC 4513 GGTACC AspI 156 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 2446 GACGAAGTC BalI 386 TGGCCA TGG^CCA 4593 TGGCCA BanI 298 GGTACC G^G[CT][GA]CC 4513 GGTACC BbvCI 198 CCTCAGC CCTCAGC(-5/-2) 2422 GCTGAGG BclI 2976 TGATCA T^GATCA 4005 TGATCA BglII 290 AGATCT A^GATCT 2482 AGATCT BlpI 799 GCTGAGC GC^T[ACGT]AGC 2775 GCTCAGC Bpu1102I 799 GCTGAGC GC^T[ACGT]AGC 2775 GCTCAGC BsaAI 403 CACGTG [CT]AC^GT[GA] 749 CACGTA BsgI 3836 CTGCAC GTGCAG(16/14) 3271 GTGCAG BsmBI 619 GAGACG CGTCTC(1/5) 1174 GAGACG BspMI 3777 GCAGGT ACCTGC(4/8) 4146 GCAGGT BstAPI 1180 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3448 GCACCAGATGC CelII 799 GCTGAGC GC^T[ACGT]AGC 2775 GCTCAGC DsaI 1001 CCACGG C^C[GA][CT]GG 3105 CCATGG EaeI 386 TGGCCA [CT]^GGCC[GA] 4593 TGGCCA EcoNI 309 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2355 CCTAAAGCAGG FbaI 2976 TGATCA T^GATCA 4005 TGATCA HincII 47 GTCAAC GT[CT]^[GA]AC 3310 GTCAAC HindII 47 GTCAAC GT[CT]^[GA]AC 3310 GTCAAC KpnI 298 GGTACC GGTAC^C 4513 GGTACC MluNI 386 TGGCCA TGG^CCA 4593 TGGCCA MscI 386 TGGCCA TGG^CCA 4593 TGGCCA PflFI 156 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 2446 GACGAAGTC Psp1406I 2410 AACGTT AA^CGTT 4265 AACGTT SapI 1621 GAAGAGC GCTCTTC(1/4) 2278 GAAGAGC SmaI 437 CCCGGG CCC^GGG 956 CCCGGG SphI 639 GCATGC GCATG^C 1050 GCATGC Tth111I 156 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 2446 GACGAAGTC XbaI 2700 TCTAGA T^CTAGA 4375 TCTAGA XmaCI 437 CCCGGG C^CCGGG 956 CCCGGG XmaI 437 CCCGGG C^CCGGG 956 CCCGGG XmnI 4367 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4549 GAATTGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwNI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DrdI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII