##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccIII AclI AgeI Aor51HI AscI
AseI AsnI AspI AviII AvrII
BciVI BclI BlnI BseAI BsiWI
BsmBI BspDI BspEI BsrDI Bst1107I
BstBI BstZ17I ClaI EagI EclXI
Eco47III Eco52I EcoT22I FbaI FseI
FspI MfeI MluI MroI MunI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 2811 GGTACC G^GTACC
AccI 4842 GTCGAC GT^[AC][GT]AC
AflII 496 CTTAAG C^TTAAG
Asp718I 2811 GGTACC G^GTACC
BbrPI 2179 CACGTG CAC^GTG
BbvCI 5567 GCTGAGG CCTCAGC(-5/-2)
BfrI 496 CTTAAG C^TTAAG
BsaAI 2179 CACGTG [CT]AC^GT[GA]
BsaWI 1199 ACCGGA [AT]^CCGG[AT]
BsmI 4577 GAATGC GAATGC(1/-1)
BspMI 3271 ACCTGC ACCTGC(4/8)
BssHII 1902 GCGCGC G^CGCGC
BssSI 2460 CTCGTG CACGAG(-5/-1)
BstAPI 1386 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2879 GGTGACC G^GT[ACGT]ACC
BstPI 2879 GGTGACC G^GT[ACGT]ACC
CpoI 76 CGGTCCG CG^G[AT]CCG
DraIII 2284 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2796 GACACCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2879 GGTGACC G^GT[ACGT]ACC
EcoRV 2139 GATATC GAT^ATC
HpaI 4956 GTTAAC GTT^AAC
KpnI 2811 GGTACC GGTAC^C
NdeI 4779 CATATG CA^TATG
PmaCI 2179 CACGTG CAC^GTG
PmlI 2179 CACGTG CAC^GTG
PshAI 2399 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 884 CGATCG CGAT^CG
RsrII 76 CGGTCCG CG^G[AT]CCG
SalI 4842 GTCGAC G^TCGAC
SapI 3825 GAAGAGC GCTCTTC(1/4)
ScaI 5316 AGTACT AGT^ACT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 1750 GACGTC GACGT^C
2158 GACGTC
ApaI 238 GGGCCC GGGCC^C
2862 GGGCCC
Asp700I 4192 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4856 GAAGGTGTTC
BbsI 250 GAAGAC GAAGAC(2/6)
41 GTCTTC
BglI 2489 GCCCGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4506 GCCCATGGGGC
BpuAI 250 GAAGAC GAAGAC(2/6)
41 GTCTTC
BsiEI 884 CGATCG CG[GA][CT]^CG
2774 CGGTCG
BspHI 1922 TCATGA T^CATGA
5013 TCATGA
BspLU11I 4627 ACATGT A^CATGT
4846 ACATGT
EcoRI 4869 GAATTC G^AATTC
5180 GAATTC
KspI 2344 CCGCGG CCGC^GG
2711 CCGCGG
NaeI 21 GCCGGC GCC^GGC
3704 GCCGGC
NgoMIV 21 GCCGGC G^CCGGC
3704 GCCGGC
NspI 4627 ACATGT [GA]CATG^[CT]
4846 ACATGT
RcaI 1922 TCATGA T^CATGA
5013 TCATGA
SacII 2344 CCGCGG CCGC^GG
2711 CCGCGG
SexAI 1667 ACCTGGT A^CC[AT]GGT
2285 ACCTGGT
StuI 1240 AGGCCT AGG^CCT
4394 AGGCCT
XmnI 4192 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4856 GAAGGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI AocI ApaLI ApoI AspEI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbvI BcgI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrBI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI