################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AccIII AclI AgeI Aor51HI AscI AspI AviII BcgI BciVI BglII BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DrdI Eco47III EcoO65I FseI FspI MamI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SalI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SspBI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AatII 5255 GACGTC GACGT^C Acc65I 405 GGTACC G^GTACC AflII 3768 CTTAAG C^TTAAG AhdI 4979 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 1231 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 405 GGTACC G^GTACC AspEI 4979 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 37 CTCGGG C^[CT]CG[GA]G AvrII 1882 CCTAGG C^CTAGG BalI 3939 TGGCCA TGG^CCA BamHI 5097 GGATCC G^GATCC BbvCI 1495 GCTGAGG CCTCAGC(-5/-2) BfrI 3768 CTTAAG C^TTAAG BlnI 1882 CCTAGG C^CTAGG BsiEI 115 CGGCCG CG[GA][CT]^CG BsoBI 37 CTCGGG C^[CT]CG[GA]G BspLU11I 3891 ACATGT A^CATGT BstAPI 163 GCAGTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraI 2007 TTTAAA TTT^AAA DraIII 3264 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 115 CGGCCG C^GGCCG Eam1105I 4979 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 115 CGGCCG C^GGCCG Eco52I 115 CGGCCG C^GGCCG EcoRI 3876 GAATTC G^AATTC KpnI 405 GGTACC GGTAC^C MluNI 3939 TGGCCA TGG^CCA MscI 3939 TGGCCA TGG^CCA PacI 1622 TTAATTAA TTAAT^TAA SapI 1000 GAAGAGC GCTCTTC(1/4) SbfI 773 CCTGCAGG CCTGCA^GG Sse8387I 773 CCTGCAGG CCTGCA^GG XbaI 2556 TCTAGA T^CTAGA XmnI 1231 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 542 GTCTAC GT^[AC][GT]AC 4647 GTCTAC AflIII 1694 ACGTGT A^C[GA][CT]GT 3891 ACATGT BbeI 10 GGCGCC GGCGC^C 214 GGCGCC BbrPI 1693 CACGTG CAC^GTG 2346 CACGTG BbsI 4625 GAAGAC GAAGAC(2/6) 898 GTCTTC BcnI 108 CCCGG CC^[GC]GG 113 CCCGG BpuAI 4625 GAAGAC GAAGAC(2/6) 898 GTCTTC BsaI 764 GAGACC GGTCTC(1/5) 3773 GAGACC BspHI 1239 TCATGA T^CATGA 4524 TCATGA BsrFI 408 ACCGGC [GA]^CCGG[CT] 555 GCCGGT BstXI 1482 CCAGAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3113 CCATCACACTGG BstYI 3293 GGATCT [GA]^GATC[CT] 5097 GGATCC Cfr10I 408 ACCGGC [GA]^CCGG[CT] 555 GCCGGT EcoNI 445 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2676 CCTCCCTCAGG EcoRV 2870 GATATC GAT^ATC 3575 GATATC EcoT22I 5236 ATGCAT ATGCA^T 5413 ATGCAT HindIII 2565 AAGCTT A^AGCTT 2903 AAGCTT HpaI 2067 GTTAAC GTT^AAC 4568 GTTAAC KasI 10 GGCGCC G^GCGCC 214 GGCGCC MflI 3293 GGATCT [GA]^GATC[CT] 5097 GGATCC NarI 10 GGCGCC GG^CGCC 214 GGCGCC NciI 108 CCCGG CC^[GC]GG 113 CCCGG NdeI 632 CATATG CA^TATG 2191 CATATG NsiI 5236 ATGCAT ATGCA^T 5413 ATGCAT PflMI 196 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4203 CCAGGCTGTGG PmaCI 1693 CACGTG CAC^GTG 2346 CACGTG PmlI 1693 CACGTG CAC^GTG 2346 CACGTG RcaI 1239 TCATGA T^CATGA 4524 TCATGA SfoI 10 GGCGCC GGC^GCC 214 GGCGCC SphI 1184 GCATGC GCATG^C 3979 GCATGC SspI 1244 AATATT AAT^ATT 3352 AATATT TaqI 177 TCGA T^CGA 1988 TCGA TthHB8I 177 TCGA T^CGA 1988 TCGA Van91I 196 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4203 CCAGGCTGTGG XhoII 3293 GGATCT [GA]^GATC[CT] 5097 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AseI AsnI AspHI AvaII BanI BanII BbvI BclI BfaI BglI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BssSI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI