##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccIII AclI AgeI Aor51HI AscI
AspI AviII BcgI BciVI BglII
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DrdI Eco47III EcoO65I FseI FspI
MamI MfeI MluI MroI MunI
NaeI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PinAI PmeI
PshAI Psp1406I PvuI RsrII SalI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspBI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 5255 GACGTC GACGT^C
Acc65I 405 GGTACC G^GTACC
AflII 3768 CTTAAG C^TTAAG
AhdI 4979 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1231 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 405 GGTACC G^GTACC
AspEI 4979 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 37 CTCGGG C^[CT]CG[GA]G
AvrII 1882 CCTAGG C^CTAGG
BalI 3939 TGGCCA TGG^CCA
BamHI 5097 GGATCC G^GATCC
BbvCI 1495 GCTGAGG CCTCAGC(-5/-2)
BfrI 3768 CTTAAG C^TTAAG
BlnI 1882 CCTAGG C^CTAGG
BsiEI 115 CGGCCG CG[GA][CT]^CG
BsoBI 37 CTCGGG C^[CT]CG[GA]G
BspLU11I 3891 ACATGT A^CATGT
BstAPI 163 GCAGTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 2007 TTTAAA TTT^AAA
DraIII 3264 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 115 CGGCCG C^GGCCG
Eam1105I 4979 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 115 CGGCCG C^GGCCG
Eco52I 115 CGGCCG C^GGCCG
EcoRI 3876 GAATTC G^AATTC
KpnI 405 GGTACC GGTAC^C
MluNI 3939 TGGCCA TGG^CCA
MscI 3939 TGGCCA TGG^CCA
PacI 1622 TTAATTAA TTAAT^TAA
SapI 1000 GAAGAGC GCTCTTC(1/4)
SbfI 773 CCTGCAGG CCTGCA^GG
Sse8387I 773 CCTGCAGG CCTGCA^GG
XbaI 2556 TCTAGA T^CTAGA
XmnI 1231 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 542 GTCTAC GT^[AC][GT]AC
4647 GTCTAC
AflIII 1694 ACGTGT A^C[GA][CT]GT
3891 ACATGT
BbeI 10 GGCGCC GGCGC^C
214 GGCGCC
BbrPI 1693 CACGTG CAC^GTG
2346 CACGTG
BbsI 4625 GAAGAC GAAGAC(2/6)
898 GTCTTC
BcnI 108 CCCGG CC^[GC]GG
113 CCCGG
BpuAI 4625 GAAGAC GAAGAC(2/6)
898 GTCTTC
BsaI 764 GAGACC GGTCTC(1/5)
3773 GAGACC
BspHI 1239 TCATGA T^CATGA
4524 TCATGA
BsrFI 408 ACCGGC [GA]^CCGG[CT]
555 GCCGGT
BstXI 1482 CCAGAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3113 CCATCACACTGG
BstYI 3293 GGATCT [GA]^GATC[CT]
5097 GGATCC
Cfr10I 408 ACCGGC [GA]^CCGG[CT]
555 GCCGGT
EcoNI 445 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2676 CCTCCCTCAGG
EcoRV 2870 GATATC GAT^ATC
3575 GATATC
EcoT22I 5236 ATGCAT ATGCA^T
5413 ATGCAT
HindIII 2565 AAGCTT A^AGCTT
2903 AAGCTT
HpaI 2067 GTTAAC GTT^AAC
4568 GTTAAC
KasI 10 GGCGCC G^GCGCC
214 GGCGCC
MflI 3293 GGATCT [GA]^GATC[CT]
5097 GGATCC
NarI 10 GGCGCC GG^CGCC
214 GGCGCC
NciI 108 CCCGG CC^[GC]GG
113 CCCGG
NdeI 632 CATATG CA^TATG
2191 CATATG
NsiI 5236 ATGCAT ATGCA^T
5413 ATGCAT
PflMI 196 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4203 CCAGGCTGTGG
PmaCI 1693 CACGTG CAC^GTG
2346 CACGTG
PmlI 1693 CACGTG CAC^GTG
2346 CACGTG
RcaI 1239 TCATGA T^CATGA
4524 TCATGA
SfoI 10 GGCGCC GGC^GCC
214 GGCGCC
SphI 1184 GCATGC GCATG^C
3979 GCATGC
SspI 1244 AATATT AAT^ATT
3352 AATATT
TaqI 177 TCGA T^CGA
1988 TCGA
TthHB8I 177 TCGA T^CGA
1988 TCGA
Van91I 196 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4203 CCAGGCTGTGG
XhoII 3293 GGATCT [GA]^GATC[CT]
5097 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AseI AsnI
AspHI AvaII BanI BanII BbvI
BclI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BssSI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PshBI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI