##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AlwNI AocI Aor51HI AscI AspI
AviII BbeI BbrPI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII CfoI
Cfr10I CpoI EagI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV FseI
FspI HaeII HhaI Hin1I HinP1I
HpaI KasI KspI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AflII 5501 CTTAAG C^TTAAG
AhdI 2654 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 800 GTGCAC G^TGCAC
ApaI 3591 GGGCCC GGGCC^C
ApaLI 800 GTGCAC G^TGCAC
AspEI 2654 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2302 CCTAGG C^CTAGG
BclI 4884 TGATCA T^GATCA
BfrI 5501 CTTAAG C^TTAAG
BglI 2142 GCCACTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2587 AGATCT A^GATCT
BlnI 2302 CCTAGG C^CTAGG
BspDI 1780 ATCGAT AT^CGAT
BspMI 3671 GCAGGT ACCTGC(4/8)
ClaI 1780 ATCGAT AT^CGAT
DrdI 2000 GACAATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2654 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3529 CCTAGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1169 ATGCAT ATGCA^T
FbaI 4884 TGATCA T^GATCA
HindIII 2508 AAGCTT A^AGCTT
MfeI 2002 CAATTG C^AATTG
MluI 2621 ACGCGT A^CGCGT
MunI 2002 CAATTG C^AATTG
NsiI 1169 ATGCAT ATGCA^T
PpuMI 5530 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 390 GAGCTC GAGCT^C
SmaI 1219 CCCGGG CCC^GGG
SpeI 1318 ACTAGT A^CTAGT
XmaCI 1219 CCCGGG C^CCGGG
XmaI 1219 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 312 GGTACC G^GTACC
605 GGTACC
AccII 859 CGCG CG^CG
2622 CGCG
AseI 4876 ATTAAT AT^TAAT
5547 ATTAAT
AsnI 4876 ATTAAT AT^TAAT
5547 ATTAAT
Asp718I 312 GGTACC G^GTACC
605 GGTACC
BamHI 655 GGATCC G^GATCC
5075 GGATCC
BsaBI 850 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4165 GATAGGCATC
BstUI 859 CGCG CG^CG
2622 CGCG
DraII 3590 GGGGCCC [GA]G^G[ACGT]CC[CT]
5530 GGGTCCC
DraIII 1848 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3560 CACCCAGTG
EcoO109I 3590 GGGGCCC [GA]G^G[ACGT]CC[CT]
5530 GGGTCCC
FauI 2397 CCCGC CCCGC(4/6)
860 GCGGG
KpnI 312 GGTACC GGTAC^C
605 GGTACC
MamI 850 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4165 GATAGGCATC
MvnI 859 CGCG CG^CG
2622 CGCG
PshBI 4876 ATTAAT AT^TAAT
5547 ATTAAT
PstI 2919 CTGCAG CTGCA^G
3317 CTGCAG
StuI 1911 AGGCCT AGG^CCT
3461 AGGCCT
XcmI 1926 CCAACCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4068 CCACTGGAGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BsrDI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI