##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccIII AflIII AgeI Alw44I
AlwI ApaI ApaLI AscI AseI
AsnI AviII BamHI BbeI BbrPI
BcgI BclI BglII BsaBI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspLU11I BstAPI BstBI BstYI
ClaI CpoI DraIII EagI EclXI
Eco52I EcoRV EcoT22I FbaI FseI
FspI KasI KspI MamI MflI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI Sse8387I SwaI
Van91I XhoI XhoII
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 892 GGTACC G^GTACC
AclI 2449 AACGTT AA^CGTT
AcyI 4538 GGCGTC G[GA]^CG[CT]C
AflII 5281 CTTAAG C^TTAAG
AhdI 132 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 107 AGCGCT AGC^GCT
Asp700I 2045 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 892 GGTACC G^GTACC
AspEI 132 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2284 CCCGGG C^[CT]CG[GA]G
AvrII 2606 CCTAGG C^CTAGG
BciVI 2291 GTATCC GTATCC(6/5)
BfrI 5281 CTTAAG C^TTAAG
BglI 3266 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2606 CCTAGG C^CTAGG
BsaHI 4538 GGCGTC G[GA]^CG[CT]C
BsoBI 2284 CCCGGG C^[CT]CG[GA]G
Bsp1407I 5294 TGTACA T^GTACA
BsrFI 1156 GCCGGT [GA]^CCGG[CT]
BsrGI 5294 TGTACA T^GTACA
BssHII 5401 GCGCGC G^CGCGC
BstEII 4726 GGTCACC G^GT[ACGT]ACC
BstPI 4726 GGTCACC G^GT[ACGT]ACC
Cfr10I 1156 GCCGGT [GA]^CCGG[CT]
DraI 5160 TTTAAA TTT^AAA
Eam1105I 132 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 107 AGCGCT AGC^GCT
EcoO65I 4726 GGTCACC G^GT[ACGT]ACC
EcoRI 1026 GAATTC G^AATTC
HaeII 107 AGCGCT [GA]GCGC^[CT]
Hin1I 4538 GGCGTC G[GA]^CG[CT]C
KpnI 892 GGTACC GGTAC^C
MfeI 279 CAATTG C^AATTG
MunI 279 CAATTG C^AATTG
NcoI 3429 CCATGG C^CATGG
NdeI 3057 CATATG CA^TATG
Psp1406I 2449 AACGTT AA^CGTT
SacI 84 GAGCTC GAGCT^C
ScaI 5016 AGTACT AGT^ACT
SexAI 4891 ACCTGGT A^CC[AT]GGT
SmaI 2284 CCCGGG CCC^GGG
SnaBI 597 TACGTA TAC^GTA
SpeI 5139 ACTAGT A^CTAGT
SphI 4804 GCATGC GCATG^C
SspBI 5294 TGTACA T^GTACA
TaqI 1764 TCGA T^CGA
TthHB8I 1764 TCGA T^CGA
XcmI 4639 CCATGCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2284 CCCGGG C^CCGGG
XmaI 2284 CCCGGG C^CCGGG
XmnI 2045 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AocI 4479 CCTCAGG CC^T[ACGT]AGG
4968 CCTGAGG
AspI 586 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5060 GACGGTGTC
BbvCI 1237 CCTCAGC CCTCAGC(-5/-2)
4362 CCTCAGC
BspHI 677 TCATGA T^CATGA
5177 TCATGA
BsrBI 1169 CCGCTC CCGCTC(-3/-3)
4555 CCGCTC
BssSI 4528 CACGAG CACGAG(-5/-1)
2485 CTCGTG
Bsu36I 4479 CCTCAGG CC^T[ACGT]AGG
4968 CCTGAGG
DrdI 396 GACAGGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1927 GACAGTTTTGTC
Eco81I 4479 CCTCAGG CC^T[ACGT]AGG
4968 CCTGAGG
HpaI 2175 GTTAAC GTT^AAC
4571 GTTAAC
PflFI 586 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5060 GACGGTGTC
RcaI 677 TCATGA T^CATGA
5177 TCATGA
SapI 4984 GAAGAGC GCTCTTC(1/4)
3138 GCTCTTC
SspI 425 AATATT AAT^ATT
3132 AATATT
Tth111I 586 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5060 GACGGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwNI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI Bst1107I BstF5I
BstNI BstUI BstXI BstZ17I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XbaI