################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AccIII AflIII AgeI Alw44I AlwI ApaI ApaLI AscI AseI AsnI AviII BamHI BbeI BbrPI BcgI BclI BglII BsaBI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspLU11I BstAPI BstBI BstYI ClaI CpoI DraIII EagI EclXI Eco52I EcoRV EcoT22I FbaI FseI FspI KasI KspI MamI MflI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI Sse8387I SwaI Van91I XhoI XhoII ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 892 GGTACC G^GTACC AclI 2449 AACGTT AA^CGTT AcyI 4538 GGCGTC G[GA]^CG[CT]C AflII 5281 CTTAAG C^TTAAG AhdI 132 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 107 AGCGCT AGC^GCT Asp700I 2045 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 892 GGTACC G^GTACC AspEI 132 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 2284 CCCGGG C^[CT]CG[GA]G AvrII 2606 CCTAGG C^CTAGG BciVI 2291 GTATCC GTATCC(6/5) BfrI 5281 CTTAAG C^TTAAG BglI 3266 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2606 CCTAGG C^CTAGG BsaHI 4538 GGCGTC G[GA]^CG[CT]C BsoBI 2284 CCCGGG C^[CT]CG[GA]G Bsp1407I 5294 TGTACA T^GTACA BsrFI 1156 GCCGGT [GA]^CCGG[CT] BsrGI 5294 TGTACA T^GTACA BssHII 5401 GCGCGC G^CGCGC BstEII 4726 GGTCACC G^GT[ACGT]ACC BstPI 4726 GGTCACC G^GT[ACGT]ACC Cfr10I 1156 GCCGGT [GA]^CCGG[CT] DraI 5160 TTTAAA TTT^AAA Eam1105I 132 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 107 AGCGCT AGC^GCT EcoO65I 4726 GGTCACC G^GT[ACGT]ACC EcoRI 1026 GAATTC G^AATTC HaeII 107 AGCGCT [GA]GCGC^[CT] Hin1I 4538 GGCGTC G[GA]^CG[CT]C KpnI 892 GGTACC GGTAC^C MfeI 279 CAATTG C^AATTG MunI 279 CAATTG C^AATTG NcoI 3429 CCATGG C^CATGG NdeI 3057 CATATG CA^TATG Psp1406I 2449 AACGTT AA^CGTT SacI 84 GAGCTC GAGCT^C ScaI 5016 AGTACT AGT^ACT SexAI 4891 ACCTGGT A^CC[AT]GGT SmaI 2284 CCCGGG CCC^GGG SnaBI 597 TACGTA TAC^GTA SpeI 5139 ACTAGT A^CTAGT SphI 4804 GCATGC GCATG^C SspBI 5294 TGTACA T^GTACA TaqI 1764 TCGA T^CGA TthHB8I 1764 TCGA T^CGA XcmI 4639 CCATGCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 2284 CCCGGG C^CCGGG XmaI 2284 CCCGGG C^CCGGG XmnI 2045 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AocI 4479 CCTCAGG CC^T[ACGT]AGG 4968 CCTGAGG AspI 586 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5060 GACGGTGTC BbvCI 1237 CCTCAGC CCTCAGC(-5/-2) 4362 CCTCAGC BspHI 677 TCATGA T^CATGA 5177 TCATGA BsrBI 1169 CCGCTC CCGCTC(-3/-3) 4555 CCGCTC BssSI 4528 CACGAG CACGAG(-5/-1) 2485 CTCGTG Bsu36I 4479 CCTCAGG CC^T[ACGT]AGG 4968 CCTGAGG DrdI 396 GACAGGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1927 GACAGTTTTGTC Eco81I 4479 CCTCAGG CC^T[ACGT]AGG 4968 CCTGAGG HpaI 2175 GTTAAC GTT^AAC 4571 GTTAAC PflFI 586 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5060 GACGGTGTC RcaI 677 TCATGA T^CATGA 5177 TCATGA SapI 4984 GAAGAGC GCTCTTC(1/4) 3138 GCTCTTC SspI 425 AATATT AAT^ATT 3132 AATATT Tth111I 586 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5060 GACGGTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwNI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI Bst1107I BstF5I BstNI BstUI BstXI BstZ17I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI