##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AclI AocI Aor51HI ApaI
AscI Asp718I AviII BamHI BglII
BlpI Bpu1102I BsiEI BsiWI BspDI
BsrBI BssHII BstBI BstEII BstPI
Bsu36I CelII ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I FseI FspI HincII
HindII HpaI KpnI KspI MluI
NheI NotI NruI NspV PacI
PaeR7I PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 119 GACGTC GACGT^C
AccIII 39 TCCGGA T^CCGGA
AflII 5481 CTTAAG C^TTAAG
AgeI 1415 ACCGGT A^CCGGT
AhdI 1705 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3235 GTGCAC G^TGCAC
ApaLI 3235 GTGCAC G^TGCAC
AseI 890 ATTAAT AT^TAAT
AsnI 890 ATTAAT AT^TAAT
Asp700I 590 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1705 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5756 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 200 GGCGCC GGCGC^C
BbrPI 5452 CACGTG CAC^GTG
BfrI 5481 CTTAAG C^TTAAG
BglI 178 GCCCTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 5452 CACGTG [CT]AC^GT[GA]
BsaBI 754 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 39 TCCGGA T^CCGGA
BspEI 39 TCCGGA T^CCGGA
BssSI 5547 CACGAG CACGAG(-5/-1)
DsaI 3941 CCATGG C^C[GA][CT]GG
Eam1105I 1705 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 4266 GATATC GAT^ATC
HgaI 4768 GACGC GACGC(5/10)
HindIII 4872 AAGCTT A^AGCTT
KasI 200 GGCGCC G^GCGCC
MamI 754 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 39 TCCGGA T^CCGGA
NaeI 174 GCCGGC GCC^GGC
NarI 200 GGCGCC GG^CGCC
NcoI 3941 CCATGG C^CATGG
NgoMIV 174 GCCGGC G^CCGGC
PflFI 5756 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3216 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1415 ACCGGT A^CCGGT
PmaCI 5452 CACGTG CAC^GTG
PmlI 5452 CACGTG CAC^GTG
PshBI 890 ATTAAT AT^TAAT
SacI 1465 GAGCTC GAGCT^C
SexAI 453 ACCTGGT A^CC[AT]GGT
SfoI 200 GGCGCC GGC^GCC
Tth111I 5756 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3216 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4278 TCTAGA T^CTAGA
XmnI 590 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 119 GACGTC G[GA]^CG[CT]C
200 GGCGCC
AflIII 1762 ACATGT A^C[GA][CT]GT
5051 ACATGT
AspHI 1465 GAGCTC G[AT]GC[AT]^C
3235 GTGCAC
AvrII 1881 CCTAGG C^CTAGG
4736 CCTAGG
BcgI 3194 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
116 GCAGACGTCTCG
BciVI 3494 GGATAC GTATCC(6/5)
4061 GGATAC
BclI 756 TGATCA T^GATCA
4434 TGATCA
BlnI 1881 CCTAGG C^CTAGG
4736 CCTAGG
BsaHI 119 GACGTC G[GA]^CG[CT]C
200 GGCGCC
BsaWI 39 TCCGGA [AT]^CCGG[AT]
1415 ACCGGT
BsiHKAI 1465 GAGCTC G[AT]GC[AT]^C
3235 GTGCAC
BspLU11I 1762 ACATGT A^CATGT
5051 ACATGT
Bst1107I 3453 GTATAC GTA^TAC
5026 GTATAC
BstAPI 4713 GCACCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5236 GCAAGATGTGC
BstZ17I 3453 GTATAC GTA^TAC
5026 GTATAC
EcoNI 3725 CCTAGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4731 CCTCGCCTAGG
FbaI 756 TGATCA T^GATCA
4434 TGATCA
HaeII 53 GGCGCT [GA]GCGC^[CT]
200 GGCGCC
Hin1I 119 GACGTC G[GA]^CG[CT]C
200 GGCGCC
MfeI 1640 CAATTG C^AATTG
5388 CAATTG
MunI 1640 CAATTG C^AATTG
5388 CAATTG
PpuMI 300 AGGACCT [GA]G^G[AT]CC[CT]
5062 AGGTCCT
PstI 2849 CTGCAG CTGCA^G
3154 CTGCAG
SspI 986 AATATT AAT^ATT
2520 AATATT
TaqI 621 TCGA T^CGA
1126 TCGA
TthHB8I 621 TCGA T^CGA
1126 TCGA
XcmI 2301 CCAAAACTACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5149 CCATTATTGACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspHI BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
NsiI NspI PleI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI SspBI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmaCI
XmaI