##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AclI AhdI ApaI AspEI AviII
AvrII BamHI BbrPI BciVI BclI
BlnI BsaAI BsiWI BsmBI BspDI
BsrBI BssSI Bst1107I BstZ17I ClaI
CpoI Eam1105I EcoRV FbaI FseI
FspI NheI NotI NruI PacI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SalI SbfI SfiI SnaBI
SphI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 2194 GACGTC GACGT^C
Acc65I 5784 GGTACC G^GTACC
AccI 2926 GTCTAC GT^[AC][GT]AC
AccIII 771 TCCGGA T^CCGGA
AgeI 2209 ACCGGT A^CCGGT
Alw44I 2950 GTGCAC G^TGCAC
Aor51HI 1872 AGCGCT AGC^GCT
ApaLI 2950 GTGCAC G^TGCAC
AscI 648 GGCGCGCC GG^CGCGCC
Asp718I 5784 GGTACC G^GTACC
AspI 2877 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2963 TGGCCA TGG^CCA
BbsI 2646 GAAGAC GAAGAC(2/6)
BcgI 454 GCAGGAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 460 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 780 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 780 GCTCAGC GC^T[ACGT]AGC
BpuAI 2646 GAAGAC GAAGAC(2/6)
BseAI 771 TCCGGA T^CCGGA
BspEI 771 TCCGGA T^CCGGA
BspHI 3149 TCATGA T^CATGA
BsrDI 2348 GCAATG GCAATG(2/0)
BssHII 649 GCGCGC G^CGCGC
BstBI 4967 TTCGAA TT^CGAA
CelII 780 GCTCAGC GC^T[ACGT]AGC
DraIII 2944 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1314 GACGAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1872 AGCGCT AGC^GCT
EcoNI 941 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3602 GAATTC G^AATTC
EcoT22I 154 ATGCAT ATGCA^T
KpnI 5784 GGTACC GGTAC^C
MluI 190 ACGCGT A^CGCGT
MluNI 2963 TGGCCA TGG^CCA
MroI 771 TCCGGA T^CCGGA
MscI 2963 TGGCCA TGG^CCA
NaeI 533 GCCGGC GCC^GGC
NcoI 965 CCATGG C^CATGG
NgoMIV 533 GCCGGC G^CCGGC
NsiI 154 ATGCAT ATGCA^T
NspV 4967 TTCGAA TT^CGAA
PaeR7I 563 CTCGAG C^TCGAG
PflFI 2877 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 5423 CCATACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2209 ACCGGT A^CCGGT
RcaI 3149 TCATGA T^CATGA
SapI 4207 GCTCTTC GCTCTTC(1/4)
SexAI 1634 ACCTGGT A^CC[AT]GGT
SfuI 4967 TTCGAA TT^CGAA
SgrAI 532 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 4548 ACTAGT A^CTAGT
Tth111I 2877 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 5423 CCATACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 563 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 1680 CTTAAG C^TTAAG
2293 CTTAAG
AocI 2817 CCTCAGG CC^T[ACGT]AGG
3241 CCTGAGG
Asp700I 1329 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3692 GAACTTGTTC
BbeI 527 GGCGCC GGCGC^C
547 GGCGCC
BfrI 1680 CTTAAG C^TTAAG
2293 CTTAAG
BsaBI 153 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4414 GATAAGCATC
BsmI 2599 GAATGC GAATGC(1/-1)
1838 GCATTC
Bsp1407I 3035 TGTACA T^GTACA
5719 TGTACA
BsrGI 3035 TGTACA T^GTACA
5719 TGTACA
Bsu36I 2817 CCTCAGG CC^T[ACGT]AGG
3241 CCTGAGG
EagI 670 CGGCCG C^GGCCG
883 CGGCCG
EclXI 670 CGGCCG C^GGCCG
883 CGGCCG
Eco52I 670 CGGCCG C^GGCCG
883 CGGCCG
Eco81I 2817 CCTCAGG CC^T[ACGT]AGG
3241 CCTGAGG
HindIII 1196 AAGCTT A^AGCTT
4686 AAGCTT
HpaI 3919 GTTAAC GTT^AAC
3928 GTTAAC
KasI 527 GGCGCC G^GCGCC
547 GGCGCC
KspI 676 CCGCGG CCGC^GG
2915 CCGCGG
MamI 153 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4414 GATAAGCATC
NarI 527 GGCGCC GG^CGCC
547 GGCGCC
NdeI 1648 CATATG CA^TATG
5544 CATATG
PshAI 3350 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3484 GACGCAGGTC
PvuII 1972 CAGCTG CAG^CTG
5197 CAGCTG
SacI 681 GAGCTC GAGCT^C
2701 GAGCTC
SacII 676 CCGCGG CCGC^GG
2915 CCGCGG
ScaI 2477 AGTACT AGT^ACT
5462 AGTACT
SfoI 527 GGCGCC GGC^GCC
547 GGCGCC
SspBI 3035 TGTACA T^GTACA
5719 TGTACA
XmnI 1329 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3692 GAACTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BglII BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshBI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI