################################## # NO CUT ENZYMES ( 44 enzymes) # ################################## AclI AhdI ApaI AspEI AviII AvrII BamHI BbrPI BciVI BclI BlnI BsaAI BsiWI BsmBI BspDI BsrBI BssSI Bst1107I BstZ17I ClaI CpoI Eam1105I EcoRV FbaI FseI FspI NheI NotI NruI PacI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI SfiI SnaBI SphI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 2194 GACGTC GACGT^C Acc65I 5784 GGTACC G^GTACC AccI 2926 GTCTAC GT^[AC][GT]AC AccIII 771 TCCGGA T^CCGGA AgeI 2209 ACCGGT A^CCGGT Alw44I 2950 GTGCAC G^TGCAC Aor51HI 1872 AGCGCT AGC^GCT ApaLI 2950 GTGCAC G^TGCAC AscI 648 GGCGCGCC GG^CGCGCC Asp718I 5784 GGTACC G^GTACC AspI 2877 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2963 TGGCCA TGG^CCA BbsI 2646 GAAGAC GAAGAC(2/6) BcgI 454 GCAGGAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 460 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 780 GCTCAGC GC^T[ACGT]AGC Bpu1102I 780 GCTCAGC GC^T[ACGT]AGC BpuAI 2646 GAAGAC GAAGAC(2/6) BseAI 771 TCCGGA T^CCGGA BspEI 771 TCCGGA T^CCGGA BspHI 3149 TCATGA T^CATGA BsrDI 2348 GCAATG GCAATG(2/0) BssHII 649 GCGCGC G^CGCGC BstBI 4967 TTCGAA TT^CGAA CelII 780 GCTCAGC GC^T[ACGT]AGC DraIII 2944 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1314 GACGAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1872 AGCGCT AGC^GCT EcoNI 941 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3602 GAATTC G^AATTC EcoT22I 154 ATGCAT ATGCA^T KpnI 5784 GGTACC GGTAC^C MluI 190 ACGCGT A^CGCGT MluNI 2963 TGGCCA TGG^CCA MroI 771 TCCGGA T^CCGGA MscI 2963 TGGCCA TGG^CCA NaeI 533 GCCGGC GCC^GGC NcoI 965 CCATGG C^CATGG NgoMIV 533 GCCGGC G^CCGGC NsiI 154 ATGCAT ATGCA^T NspV 4967 TTCGAA TT^CGAA PaeR7I 563 CTCGAG C^TCGAG PflFI 2877 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 5423 CCATACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2209 ACCGGT A^CCGGT RcaI 3149 TCATGA T^CATGA SapI 4207 GCTCTTC GCTCTTC(1/4) SexAI 1634 ACCTGGT A^CC[AT]GGT SfuI 4967 TTCGAA TT^CGAA SgrAI 532 CGCCGGCG C[GA]^CCGG[CT]G SpeI 4548 ACTAGT A^CTAGT Tth111I 2877 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 5423 CCATACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 563 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflII 1680 CTTAAG C^TTAAG 2293 CTTAAG AocI 2817 CCTCAGG CC^T[ACGT]AGG 3241 CCTGAGG Asp700I 1329 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3692 GAACTTGTTC BbeI 527 GGCGCC GGCGC^C 547 GGCGCC BfrI 1680 CTTAAG C^TTAAG 2293 CTTAAG BsaBI 153 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4414 GATAAGCATC BsmI 2599 GAATGC GAATGC(1/-1) 1838 GCATTC Bsp1407I 3035 TGTACA T^GTACA 5719 TGTACA BsrGI 3035 TGTACA T^GTACA 5719 TGTACA Bsu36I 2817 CCTCAGG CC^T[ACGT]AGG 3241 CCTGAGG EagI 670 CGGCCG C^GGCCG 883 CGGCCG EclXI 670 CGGCCG C^GGCCG 883 CGGCCG Eco52I 670 CGGCCG C^GGCCG 883 CGGCCG Eco81I 2817 CCTCAGG CC^T[ACGT]AGG 3241 CCTGAGG HindIII 1196 AAGCTT A^AGCTT 4686 AAGCTT HpaI 3919 GTTAAC GTT^AAC 3928 GTTAAC KasI 527 GGCGCC G^GCGCC 547 GGCGCC KspI 676 CCGCGG CCGC^GG 2915 CCGCGG MamI 153 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4414 GATAAGCATC NarI 527 GGCGCC GG^CGCC 547 GGCGCC NdeI 1648 CATATG CA^TATG 5544 CATATG PshAI 3350 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3484 GACGCAGGTC PvuII 1972 CAGCTG CAG^CTG 5197 CAGCTG SacI 681 GAGCTC GAGCT^C 2701 GAGCTC SacII 676 CCGCGG CCGC^GG 2915 CCGCGG ScaI 2477 AGTACT AGT^ACT 5462 AGTACT SfoI 527 GGCGCC GGC^GCC 547 GGCGCC SspBI 3035 TGTACA T^GTACA 5719 TGTACA XmnI 1329 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3692 GAACTTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BglII BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI