##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII AccII AclI AflII AgeI
AscI AviII AvrII BcgI BfrI
BlnI BsiEI BsiWI BsmBI BssHII
BstUI CpoI DrdI EagI EclXI
Eco52I EcoRV FseI FspI HgaI
HindIII KspI MluI MvnI NdeI
NotI NruI PacI PinAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SgrAI SphI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 3097 TCCGGA T^CCGGA
Aor51HI 1200 AGCGCT AGC^GCT
AspI 2976 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglI 782 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 84 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3097 TCCGGA T^CCGGA
BsgI 5436 GTGCAG GTGCAG(16/14)
BspDI 5147 ATCGAT AT^CGAT
BspEI 3097 TCCGGA T^CCGGA
BssSI 5475 CACGAG CACGAG(-5/-1)
BstBI 2872 TTCGAA TT^CGAA
BstEII 4568 GGTGACC G^GT[ACGT]ACC
BstPI 4568 GGTGACC G^GT[ACGT]ACC
ClaI 5147 ATCGAT AT^CGAT
DraIII 2662 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1200 AGCGCT AGC^GCT
EcoO65I 4568 GGTGACC G^GT[ACGT]ACC
HpaI 753 GTTAAC GTT^AAC
MamI 84 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3097 TCCGGA T^CCGGA
NaeI 101 GCCGGC GCC^GGC
NcoI 3064 CCATGG C^CATGG
NgoMIV 101 GCCGGC G^CCGGC
NheI 1236 GCTAGC G^CTAGC
NspV 2872 TTCGAA TT^CGAA
PflFI 2976 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 3889 GTTTAAAC GTTT^AAAC
PshAI 1120 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 2872 TTCGAA TT^CGAA
SmaI 482 CCCGGG CCC^GGG
SnaBI 2329 TACGTA TAC^GTA
SpeI 3978 ACTAGT A^CTAGT
StuI 642 AGGCCT AGG^CCT
Tth111I 2976 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4952 TCTAGA T^CTAGA
XmaCI 482 CCCGGG C^CCGGG
XmaI 482 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 191 GGTACC G^GTACC
3797 GGTACC
AcyI 60 GGCGCC G[GA]^CG[CT]C
299 GGCGCC
AhdI 3670 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4083 GACAATCAGTC
Alw44I 703 GTGCAC G^TGCAC
5472 GTGCAC
ApaI 283 GGGCCC GGGCC^C
840 GGGCCC
ApaLI 703 GTGCAC G^TGCAC
5472 GTGCAC
AseI 1665 ATTAAT AT^TAAT
4305 ATTAAT
AsnI 1665 ATTAAT AT^TAAT
4305 ATTAAT
Asp700I 3588 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3783 GAAAAAATTC
Asp718I 191 GGTACC G^GTACC
3797 GGTACC
AspEI 3670 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4083 GACAATCAGTC
BalI 2717 TGGCCA TGG^CCA
4525 TGGCCA
BamHI 1037 GGATCC G^GATCC
2597 GGATCC
BbeI 60 GGCGCC GGCGC^C
299 GGCGCC
BbrPI 5315 CACGTG CAC^GTG
5467 CACGTG
BbvCI 1143 CCTCAGC CCTCAGC(-5/-2)
4739 CCTCAGC
BciVI 1368 GTATCC GTATCC(6/5)
1801 GTATCC
BclI 5162 TGATCA T^GATCA
5507 TGATCA
BglII 2706 AGATCT A^GATCT
4927 AGATCT
BlpI 2319 GCTGAGC GC^T[ACGT]AGC
4176 GCTGAGC
Bpu1102I 2319 GCTGAGC GC^T[ACGT]AGC
4176 GCTGAGC
BsaHI 60 GGCGCC G[GA]^CG[CT]C
299 GGCGCC
BsaI 2266 GAGACC GGTCTC(1/5)
4122 GAGACC
BsrBI 166 GAGCGG CCGCTC(-3/-3)
2955 GAGCGG
BsrDI 6182 CATTGC GCAATG(2/0)
4221 GCAATG
BstXI 2115 CCAAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2427 CCAGAATCCTGG
CelII 2319 GCTGAGC GC^T[ACGT]AGC
4176 GCTGAGC
DsaI 1702 CCACGG C^C[GA][CT]GG
3064 CCATGG
Eam1105I 3670 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4083 GACAATCAGTC
EcoRI 584 GAATTC G^AATTC
1030 GAATTC
EcoT14I 3064 CCATGG C^C[AT][AT]GG
3241 CCAAGG
EcoT22I 490 ATGCAT ATGCA^T
3327 ATGCAT
FbaI 5162 TGATCA T^GATCA
5507 TGATCA
Hin1I 60 GGCGCC G[GA]^CG[CT]C
299 GGCGCC
HincII 753 GTTAAC GT[CT]^[GA]AC
2943 GTTGAC
HindII 753 GTTAAC GT[CT]^[GA]AC
2943 GTTGAC
KasI 60 GGCGCC G^GCGCC
299 GGCGCC
KpnI 191 GGTACC GGTAC^C
3797 GGTACC
MluNI 2717 TGGCCA TGG^CCA
4525 TGGCCA
MscI 2717 TGGCCA TGG^CCA
4525 TGGCCA
NarI 60 GGCGCC GG^CGCC
299 GGCGCC
NsiI 490 ATGCAT ATGCA^T
3327 ATGCAT
PaeR7I 2614 CTCGAG C^TCGAG
4887 CTCGAG
PflMI 897 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3680 CCAGAGAGTGG
PmaCI 5315 CACGTG CAC^GTG
5467 CACGTG
PmlI 5315 CACGTG CAC^GTG
5467 CACGTG
PshBI 1665 ATTAAT AT^TAAT
4305 ATTAAT
SfoI 60 GGCGCC GGC^GCC
299 GGCGCC
StyI 3064 CCATGG C^C[AT][AT]GG
3241 CCAAGG
Van91I 897 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3680 CCAGAGAGTGG
XhoI 2614 CTCGAG C^TCGAG
4887 CTCGAG
XmnI 3588 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3783 GAAAAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspHI BspLU11I BspMI BsrFI BsrGI
BsrI BssKI Bst1107I BstAPI BstF5I
BstNI BstYI BstZ17I Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScaI ScrFI SexAI SfaNI SfcI
SmlI SspBI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII