##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AlwNI
AocI ApaI AscI Asp718I AviII
BalI BamHI BbeI BbrPI BcgI
BcnI BsaAI BsaBI BsaI BseAI
BsiWI BsmI BspEI BspHI BssHII
BssSI Bsu36I CpoI DrdI Eco81I
EcoRV FseI FspI KasI KpnI
KspI MamI MluNI MroI MscI
NaeI NarI NciI NgoMIV NheI
NotI NruI PacI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 1441 CGCG CG^CG
AcyI 218 GGCGTC G[GA]^CG[CT]C
AflII 1557 CTTAAG C^TTAAG
AgeI 5012 ACCGGT A^CCGGT
AhdI 4580 GACTTATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4777 AGCGCT AGC^GCT
AspEI 4580 GACTTATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4502 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4065 CCTAGG C^CTAGG
BanI 4091 GGTGCC G^G[CT][GA]CC
BanII 1964 GAGCCC G[GA]GC[CT]^C
BbvCI 4780 GCTGAGG CCTCAGC(-5/-2)
BfrI 1557 CTTAAG C^TTAAG
BglI 5120 GCCCTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4065 CCTAGG C^CTAGG
BlpI 2250 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2250 GCTGAGC GC^T[ACGT]AGC
BsaHI 218 GGCGTC G[GA]^CG[CT]C
BsaWI 5012 ACCGGT [AT]^CCGG[AT]
BsiEI 3638 CGGCCG CG[GA][CT]^CG
BspLU11I 4935 ACATGT A^CATGT
BsrFI 5012 ACCGGT [GA]^CCGG[CT]
BstEII 2692 GGTTACC G^GT[ACGT]ACC
BstPI 2692 GGTTACC G^GT[ACGT]ACC
BstUI 1441 CGCG CG^CG
CelII 2250 GCTGAGC GC^T[ACGT]AGC
Cfr10I 5012 ACCGGT [GA]^CCGG[CT]
DraII 3003 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 957 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3638 CGGCCG C^GGCCG
Eam1105I 4580 GACTTATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3638 CGGCCG C^GGCCG
Eco47III 4777 AGCGCT AGC^GCT
Eco52I 3638 CGGCCG C^GGCCG
EcoO109I 3003 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 2692 GGTTACC G^GT[ACGT]ACC
EcoRI 2080 GAATTC G^AATTC
Hin1I 218 GGCGTC G[GA]^CG[CT]C
HincII 4884 GTTAAC GT[CT]^[GA]AC
HindII 4884 GTTAAC GT[CT]^[GA]AC
HpaI 4884 GTTAAC GTT^AAC
MluI 1440 ACGCGT A^CGCGT
MvnI 1441 CGCG CG^CG
PflFI 4502 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5012 ACCGGT A^CCGGT
PpuMI 3003 GGGACCC [GA]G^G[AT]CC[CT]
SapI 630 GCTCTTC GCTCTTC(1/4)
StuI 1001 AGGCCT AGG^CCT
SwaI 5355 ATTTAAAT ATTT^AAAT
Tth111I 4502 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Alw44I 966 GTGCAC G^TGCAC
4789 GTGCAC
ApaLI 966 GTGCAC G^TGCAC
4789 GTGCAC
Asp700I 5180 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5407 GAATGATTTC
AspHI 966 GTGCAC G[AT]GC[AT]^C
4789 GTGCAC
AvaI 201 CTCGAG C^[CT]CG[GA]G
3043 CTCGAG
BciVI 2175 GGATAC GTATCC(6/5)
4825 GTATCC
BglII 402 AGATCT A^GATCT
1010 AGATCT
BsiHKAI 966 GTGCAC G[AT]GC[AT]^C
4789 GTGCAC
BsoBI 201 CTCGAG C^[CT]CG[GA]G
3043 CTCGAG
BspDI 552 ATCGAT AT^CGAT
2346 ATCGAT
BspMI 679 ACCTGC ACCTGC(4/8)
4728 GCAGGT
Bst1107I 987 GTATAC GTA^TAC
2712 GTATAC
BstAPI 1768 GCAACCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4808 GCAGCTGATGC
BstBI 461 TTCGAA TT^CGAA
1348 TTCGAA
BstXI 2069 CCATCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2977 CCACAGGGATGG
BstZ17I 987 GTATAC GTA^TAC
2712 GTATAC
ClaI 552 ATCGAT AT^CGAT
2346 ATCGAT
EaeI 238 TGGCCG [CT]^GGCC[GA]
3638 CGGCCG
EarI 631 CTCTTC CTCTTC(1/4)
1215 GAAGAG
EcoNI 2460 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5166 CCTCCTATAGG
EcoT22I 526 ATGCAT ATGCA^T
1329 ATGCAT
FauI 591 GCGGG CCCGC(4/6)
1034 GCGGG
HaeII 2118 GGCGCT [GA]GCGC^[CT]
4777 AGCGCT
HapII 3637 CCGG C^CGG
5013 CCGG
HpaII 3637 CCGG C^CGG
5013 CCGG
Ksp632I 631 CTCTTC CTCTTC(1/4)
1215 GAAGAG
MfeI 508 CAATTG C^AATTG
733 CAATTG
MspI 3637 CCGG C^CGG
5013 CCGG
MunI 508 CAATTG C^AATTG
733 CAATTG
NdeI 1810 CATATG CA^TATG
4678 CATATG
NsiI 526 ATGCAT ATGCA^T
1329 ATGCAT
NspV 461 TTCGAA TT^CGAA
1348 TTCGAA
PaeR7I 201 CTCGAG C^TCGAG
3043 CTCGAG
SfuI 461 TTCGAA TT^CGAA
1348 TTCGAA
SphI 316 GCATGC GCATG^C
1921 GCATGC
XbaI 3143 TCTAGA T^CTAGA
4757 TCTAGA
XhoI 201 CTCGAG C^TCGAG
3043 CTCGAG
XmnI 5180 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5407 GAATGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AvaII BbsI BbvI BclI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I Bsp1407I BsrBI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HgaI
HhaI HinP1I HindIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII