##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AviII BlpI Bpu1102I
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BssHII BssSI BstUI CelII
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI FseI FspI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NgoMIV NotI
NruI PacI PaeR7I PmeI PshAI
Psp1406I PvuI RsrII SacI SacII
SapI SbfI SexAI SfiI SgrAI
SnaBI SphI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AcyI 5532 GGCGCC G[GA]^CG[CT]C
AflII 2129 CTTAAG C^TTAAG
AgeI 2323 ACCGGT A^CCGGT
AocI 1469 CCTGAGG CC^T[ACGT]AGG
ApaI 2514 GGGCCC GGGCC^C
AvaI 5500 CCCGGG C^[CT]CG[GA]G
AvrII 920 CCTAGG C^CTAGG
BamHI 4758 GGATCC G^GATCC
BanII 2514 GGGCCC G[GA]GC[CT]^C
BbeI 5532 GGCGCC GGCGC^C
BbrPI 425 CACGTG CAC^GTG
BbsI 5128 GAAGAC GAAGAC(2/6)
BbvCI 5341 GCTGAGG CCTCAGC(-5/-2)
BcgI 528 GCAACAGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3865 TGATCA T^GATCA
BfrI 2129 CTTAAG C^TTAAG
BglII 2267 AGATCT A^GATCT
BlnI 920 CCTAGG C^CTAGG
BpuAI 5128 GAAGAC GAAGAC(2/6)
BsaAI 425 CACGTG [CT]AC^GT[GA]
BsaHI 5532 GGCGCC G[GA]^CG[CT]C
BsaWI 2323 ACCGGT [AT]^CCGG[AT]
BsmBI 5479 GAGACG CGTCTC(1/5)
BsoBI 5500 CCCGGG C^[CT]CG[GA]G
BspMI 1827 ACCTGC ACCTGC(4/8)
BsrFI 2323 ACCGGT [GA]^CCGG[CT]
Bst1107I 458 GTATAC GTA^TAC
BstBI 5136 TTCGAA TT^CGAA
BstEII 5655 GGTAACC G^GT[ACGT]ACC
BstPI 5655 GGTAACC G^GT[ACGT]ACC
BstZ17I 458 GTATAC GTA^TAC
Bsu36I 1469 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2323 ACCGGT [GA]^CCGG[CT]
DrdI 3362 GACAATCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1375 CTCTTC CTCTTC(1/4)
Eco81I 1469 CCTGAGG CC^T[ACGT]AGG
EcoO65I 5655 GGTAACC G^GT[ACGT]ACC
EcoRI 5370 GAATTC G^AATTC
EcoRV 1370 GATATC GAT^ATC
EcoT22I 1006 ATGCAT ATGCA^T
FbaI 3865 TGATCA T^GATCA
Hin1I 5532 GGCGCC G[GA]^CG[CT]C
KasI 5532 GGCGCC G^GCGCC
Ksp632I 1375 CTCTTC CTCTTC(1/4)
NarI 5532 GGCGCC GG^CGCC
NheI 5609 GCTAGC G^CTAGC
NsiI 1006 ATGCAT ATGCA^T
NspV 5136 TTCGAA TT^CGAA
PinAI 2323 ACCGGT A^CCGGT
PmaCI 425 CACGTG CAC^GTG
PmlI 425 CACGTG CAC^GTG
SalI 776 GTCGAC G^TCGAC
SfoI 5532 GGCGCC GGC^GCC
SfuI 5136 TTCGAA TT^CGAA
SmaI 5500 CCCGGG CCC^GGG
SpeI 3542 ACTAGT A^CTAGT
StuI 3259 AGGCCT AGG^CCT
XcmI 3280 CCATAAGCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 5500 CCCGGG C^CCGGG
XmaI 5500 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspI 714 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5567 GACCCTGTC
BanI 2930 GGTGCC G^G[CT][GA]CC
5532 GGCGCC
BsaBI 2229 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2262 GATGAAGATC
BsgI 58 CTGCAC GTGCAG(16/14)
579 GTGCAG
BsmI 743 GAATGC GAATGC(1/-1)
5541 GCATTC
Bsp1407I 5684 TGTACA T^GTACA
5714 TGTACA
BsrGI 5684 TGTACA T^GTACA
5714 TGTACA
BstAPI 5484 GCAAGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5508 GCAGAGGTTGC
DraI 3203 TTTAAA TTT^AAA
5907 TTTAAA
FauI 2358 GCGGG CCCGC(4/6)
5347 GCGGG
HgaI 5481 GACGC GACGC(5/10)
774 GCGTC
HpaI 40 GTTAAC GTT^AAC
4848 GTTAAC
MaeII 426 ACGT A^CGT
609 ACGT
MamI 2229 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2262 GATGAAGATC
NcoI 130 CCATGG C^CATGG
4614 CCATGG
NdeI 3492 CATATG CA^TATG
4941 CATATG
PflFI 714 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5567 GACCCTGTC
PpuMI 1041 AGGTCCC [GA]G^G[AT]CC[CT]
4542 AGGTCCC
SspBI 5684 TGTACA T^GTACA
5714 TGTACA
Tth111I 714 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5567 GACCCTGTC
XbaI 1892 TCTAGA T^CTAGA
3107 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BalI
BbvI BciVI BcnI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI