##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AgeI AhdI AocI
AscI AspEI AspI AvrII BcgI
BciVI BlnI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BsrBI BsrGI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoRV FseI
HpaI KspI MamI MluI NdeI
NheI NotI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2938 GTCTAC GT^[AC][GT]AC
AccIII 60 TCCGGA T^CCGGA
AflII 3401 CTTAAG C^TTAAG
Aor51HI 140 AGCGCT AGC^GCT
ApaI 153 GGGCCC GGGCC^C
BamHI 345 GGATCC G^GATCC
BbeI 119 GGCGCC GGCGC^C
BbvCI 2468 CCTCAGC CCTCAGC(-5/-2)
BfrI 3401 CTTAAG C^TTAAG
BglI 2078 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 60 TCCGGA T^CCGGA
BseRI 1346 GAGGAG GAGGAG(10/8)
BsiEI 94 CGGTCG CG[GA][CT]^CG
BspEI 60 TCCGGA T^CCGGA
BssHII 194 GCGCGC G^CGCGC
BstEII 2182 GGTGACC G^GT[ACGT]ACC
BstPI 2182 GGTGACC G^GT[ACGT]ACC
Eco47III 140 AGCGCT AGC^GCT
EcoNI 2655 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2182 GGTGACC G^GT[ACGT]ACC
KasI 119 GGCGCC G^GCGCC
MfeI 4260 CAATTG C^AATTG
MroI 60 TCCGGA T^CCGGA
MunI 4260 CAATTG C^AATTG
NarI 119 GGCGCC GG^CGCC
NruI 97 TCGCGA TCG^CGA
SacI 26 GAGCTC GAGCT^C
SbfI 1469 CCTGCAGG CCTGCA^GG
ScaI 4938 AGTACT AGT^ACT
SexAI 1654 ACCAGGT A^CC[AT]GGT
SfoI 119 GGCGCC GGC^GCC
SphI 4524 GCATGC GCATG^C
Sse8387I 1469 CCTGCAGG CCTGCA^GG
XbaI 3560 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 101 GACGCC G[GA]^CG[CT]C
119 GGCGCC
Alw44I 1012 GTGCAC G^TGCAC
2520 GTGCAC
ApaLI 1012 GTGCAC G^TGCAC
2520 GTGCAC
AseI 2965 ATTAAT AT^TAAT
5024 ATTAAT
AsnI 2965 ATTAAT AT^TAAT
5024 ATTAAT
Asp700I 355 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4302 GAAAGCCTTC
AviII 946 TGCGCA TGC^GCA
1430 TGCGCA
BclI 423 TGATCA T^GATCA
3126 TGATCA
BglII 3767 AGATCT A^GATCT
4732 AGATCT
BlpI 28 GCTCAGC GC^T[ACGT]AGC
3667 GCTGAGC
Bpu1102I 28 GCTCAGC GC^T[ACGT]AGC
3667 GCTGAGC
BsaHI 101 GACGCC G[GA]^CG[CT]C
119 GGCGCC
BsaWI 60 TCCGGA [AT]^CCGG[AT]
92 TCCGGT
BssSI 1625 CACGAG CACGAG(-5/-1)
2012 CACGAG
CelII 28 GCTCAGC GC^T[ACGT]AGC
3667 GCTGAGC
EcoRI 2175 GAATTC G^AATTC
5648 GAATTC
FbaI 423 TGATCA T^GATCA
3126 TGATCA
FspI 946 TGCGCA TGC^GCA
1430 TGCGCA
Hin1I 101 GACGCC G[GA]^CG[CT]C
119 GGCGCC
HindIII 917 AAGCTT A^AGCTT
3366 AAGCTT
NaeI 178 GCCGGC GCC^GGC
1809 GCCGGC
NgoMIV 178 GCCGGC G^CCGGC
1809 GCCGGC
PshBI 2965 ATTAAT AT^TAAT
5024 ATTAAT
StuI 2359 AGGCCT AGG^CCT
3132 AGGCCT
XmnI 355 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4302 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspLU11I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII