##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Aor51HI AscI AspEI BamHI
BbeI BbrPI BglI BglII BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BspEI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstZ17I CelII Cfr10I CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FseI HaeII Hin1I KasI
KspI MluI MroI NaeI NarI
NcoI NgoMIV NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
StuI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 562 GGTACC G^GTACC
AccI 4395 GTCTAC GT^[AC][GT]AC
AccII 1359 CGCG CG^CG
Alw44I 453 GTGCAC G^TGCAC
AlwI 17 GGATC GGATC(4/5)
ApaI 1065 GGGCCC GGGCC^C
ApaLI 453 GTGCAC G^TGCAC
AseI 861 ATTAAT AT^TAAT
AsnI 861 ATTAAT AT^TAAT
Asp718I 562 GGTACC G^GTACC
AspI 2546 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1176 CTCGAG C^[CT]CG[GA]G
AviII 134 TGCGCA TGC^GCA
BbvCI 764 CCTCAGC CCTCAGC(-5/-2)
BcgI 647 CGATGATAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5911 TGATCA T^GATCA
BcnI 941 CCGGG CC^[GC]GG
BsgI 584 CTGCAC GTGCAG(16/14)
BsoBI 1176 CTCGAG C^[CT]CG[GA]G
BspDI 276 ATCGAT AT^CGAT
BspHI 3345 TCATGA T^CATGA
BstAPI 4768 GCAAGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4816 GGTAACC G^GT[ACGT]ACC
BstPI 4816 GGTAACC G^GT[ACGT]ACC
BstUI 1359 CGCG CG^CG
BstXI 5331 CCACCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 17 GGATCT [GA]^GATC[CT]
ClaI 276 ATCGAT AT^CGAT
DsaI 241 CCGTGG C^C[GA][CT]GG
EcoO65I 4816 GGTAACC G^GT[ACGT]ACC
EcoRI 3956 GAATTC G^AATTC
FauI 1423 CCCGC CCCGC(4/6)
FbaI 5911 TGATCA T^GATCA
FspI 134 TGCGCA TGC^GCA
HapII 941 CCGG C^CGG
HindIII 774 AAGCTT A^AGCTT
HpaI 2218 GTTAAC GTT^AAC
HpaII 941 CCGG C^CGG
KpnI 562 GGTACC GGTAC^C
MaeII 5440 ACGT A^CGT
MfeI 5892 CAATTG C^AATTG
MflI 17 GGATCT [GA]^GATC[CT]
MspI 941 CCGG C^CGG
MunI 5892 CAATTG C^AATTG
MvnI 1359 CGCG CG^CG
NciI 941 CCGGG CC^[GC]GG
NheI 1244 GCTAGC G^CTAGC
PaeR7I 1176 CTCGAG C^TCGAG
PflFI 2546 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 222 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 207 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 861 ATTAAT AT^TAAT
PstI 5651 CTGCAG CTGCA^G
RcaI 3345 TCATGA T^CATGA
SpeI 2913 ACTAGT A^CTAGT
SphI 1872 GCATGC GCATG^C
Tth111I 2546 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 222 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1176 CTCGAG C^TCGAG
XhoII 17 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AlwNI 2309 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
3547 CAGAATCTG
AvrII 2198 CCTAGG C^CTAGG
3619 CCTAGG
BciVI 609 GGATAC GTATCC(6/5)
3299 GTATCC
BlnI 2198 CCTAGG C^CTAGG
3619 CCTAGG
BsmBI 177 GAGACG CGTCTC(1/5)
1355 GAGACG
DraIII 456 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1716 CACATGGTG
HgaI 1357 GACGC GACGC(5/10)
1590 GACGC
SapI 3255 GCTCTTC GCTCTTC(1/4)
3758 GCTCTTC
SspI 5449 AATATT AAT^ATT
5470 AATATT
XbaI 3763 TCTAGA T^CTAGA
4574 TCTAGA
XcmI 271 CCAGCATCGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2304 CCACACAGCATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AocI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BfaI BfrI BmyI BpmI
BpuAI BsaBI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeIII MamI
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI