##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AviII BciVI BglI BsiWI BsmBI
BspDI BspLU11I BsrFI BssHII Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DrdI Eco81I
EcoO65I EcoRV FseI FspI KpnI
KspI MluI NaeI NgoMIV NotI
NruI PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 310 GTCTAC GT^[AC][GT]AC
AccII 1769 CGCG CG^CG
AccIII 1821 TCCGGA T^CCGGA
AflII 4363 CTTAAG C^TTAAG
AhdI 4144 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 102 AGCGCT AGC^GCT
AspEI 4144 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2235 CCCGGG C^[CT]CG[GA]G
AvrII 2890 CCTAGG C^CTAGG
BamHI 3583 GGATCC G^GATCC
BbeI 1593 GGCGCC GGCGC^C
BbrPI 2088 CACGTG CAC^GTG
BbvCI 4232 CCTCAGC CCTCAGC(-5/-2)
BclI 4023 TGATCA T^GATCA
BfrI 4363 CTTAAG C^TTAAG
BglII 2815 AGATCT A^GATCT
BlnI 2890 CCTAGG C^CTAGG
BsaBI 3464 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 881 GAGACC GGTCTC(1/5)
BsaWI 1821 TCCGGA [AT]^CCGG[AT]
BseAI 1821 TCCGGA T^CCGGA
BsmI 3821 GCATTC GAATGC(1/-1)
BsoBI 2235 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3524 TGTACA T^GTACA
BspEI 1821 TCCGGA T^CCGGA
BsrGI 3524 TGTACA T^GTACA
BssSI 1581 CTCGTG CACGAG(-5/-1)
BstUI 1769 CGCG CG^CG
BstXI 3720 CCATCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 3384 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 3354 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3955 CGGCCG C^GGCCG
Eam1105I 4144 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3955 CGGCCG C^GGCCG
Eco47III 102 AGCGCT AGC^GCT
Eco52I 3955 CGGCCG C^GGCCG
EcoNI 3883 CCTTCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3384 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1664 ATGCAT ATGCA^T
FauI 806 CCCGC CCCGC(4/6)
FbaI 4023 TGATCA T^GATCA
KasI 1593 GGCGCC G^GCGCC
MamI 3464 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1821 TCCGGA T^CCGGA
MvnI 1769 CGCG CG^CG
NarI 1593 GGCGCC GG^CGCC
NdeI 3638 CATATG CA^TATG
NheI 1366 GCTAGC G^CTAGC
NsiI 1664 ATGCAT ATGCA^T
PmaCI 2088 CACGTG CAC^GTG
PmlI 2088 CACGTG CAC^GTG
PpuMI 3384 AGGACCT [GA]G^G[AT]CC[CT]
SexAI 3936 ACCAGGT A^CC[AT]GGT
SfoI 1593 GGCGCC GGC^GCC
SmaI 2235 CCCGGG CCC^GGG
SphI 3496 GCATGC GCATG^C
SspBI 3524 TGTACA T^GTACA
SspI 4325 AATATT AAT^ATT
XcmI 3837 CCAGCTCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2235 CCCGGG C^CCGGG
XmaI 2235 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 621 GACGCC G[GA]^CG[CT]C
1593 GGCGCC
Asp700I 1271 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2339 GAAAGTATTC
AspHI 381 GAGCTC G[AT]GC[AT]^C
2118 GAGCTC
BanII 381 GAGCTC G[GA]GC[CT]^C
2118 GAGCTC
BbsI 559 GAAGAC GAAGAC(2/6)
2067 GAAGAC
BcgI 1558 CGATGTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
12 GCAGGGCGGTCG
BlpI 2390 GCTGAGC GC^T[ACGT]AGC
2395 GCTAAGC
BmyI 381 GAGCTC G[AGT]GC[ACT]^C
2118 GAGCTC
Bpu1102I 2390 GCTGAGC GC^T[ACGT]AGC
2395 GCTAAGC
BpuAI 559 GAAGAC GAAGAC(2/6)
2067 GAAGAC
BsaAI 2088 CACGTG [CT]AC^GT[GA]
3398 TACGTG
BsaHI 621 GACGCC G[GA]^CG[CT]C
1593 GGCGCC
BseRI 720 CTCCTC GAGGAG(10/8)
143 GAGGAG
BsiEI 18 CGGTCG CG[GA][CT]^CG
3955 CGGCCG
BsiHKAI 381 GAGCTC G[AT]GC[AT]^C
2118 GAGCTC
Bsp1286I 381 GAGCTC G[AGT]GC[ACT]^C
2118 GAGCTC
BspMI 1633 ACCTGC ACCTGC(4/8)
3074 ACCTGC
BsrBI 2132 GAGCGG CCGCTC(-3/-3)
2675 GAGCGG
BstBI 1179 TTCGAA TT^CGAA
2346 TTCGAA
BstYI 2815 AGATCT [GA]^GATC[CT]
3583 GGATCC
CelII 2390 GCTGAGC GC^T[ACGT]AGC
2395 GCTAAGC
CfoI 103 GCGC GCG^C
1594 GCGC
DraI 78 TTTAAA TTT^AAA
4330 TTTAAA
HaeII 102 AGCGCT [GA]GCGC^[CT]
1593 GGCGCC
HhaI 103 GCGC GCG^C
1594 GCGC
Hin1I 621 GACGCC G[GA]^CG[CT]C
1593 GGCGCC
HinP1I 103 GCGC G^CGC
1594 GCGC
HpaI 1432 GTTAAC GTT^AAC
4676 GTTAAC
MfeI 1701 CAATTG C^AATTG
1917 CAATTG
MflI 2815 AGATCT [GA]^GATC[CT]
3583 GGATCC
MspA1I 2280 CAGCTG C[AC]G^C[GT]G
3600 CAGCTG
MunI 1701 CAATTG C^AATTG
1917 CAATTG
NspV 1179 TTCGAA TT^CGAA
2346 TTCGAA
PflMI 1545 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1646 CCAGGGCATGG
PvuII 2280 CAGCTG CAG^CTG
3600 CAGCTG
SacI 381 GAGCTC GAGCT^C
2118 GAGCTC
ScaI 281 AGTACT AGT^ACT
4030 AGTACT
SfuI 1179 TTCGAA TT^CGAA
2346 TTCGAA
Van91I 1545 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1646 CCAGGGCATGG
XbaI 1378 TCTAGA T^CTAGA
2122 TCTAGA
XhoII 2815 AGATCT [GA]^GATC[CT]
3583 GGATCC
XmnI 1271 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2339 GAAAGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BalI BanI BbvI
BcnI BfaI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BspHI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I