##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AviII AvrII
BbeI BbrPI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PstI PvuI RsrII SacI SacII
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I SspBI
StuI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 3785 AACGTT AA^CGTT
AflII 3291 CTTAAG C^TTAAG
AflIII 41 ACGTGT A^C[GA][CT]GT
AlwNI 3550 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 2216 ATTAAT AT^TAAT
AsnI 2216 ATTAAT AT^TAAT
AspHI 67 GTGCTC G[AT]GC[AT]^C
AspI 1044 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3469 TGGCCA TGG^CCA
BamHI 104 GGATCC G^GATCC
BbvCI 3387 CCTCAGC CCTCAGC(-5/-2)
BciVI 3856 GTATCC GTATCC(6/5)
BfrI 3291 CTTAAG C^TTAAG
BsaBI 1706 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 381 GTGCAG GTGCAG(16/14)
BsiHKAI 67 GTGCTC G[AT]GC[AT]^C
BsmBI 3449 GAGACG CGTCTC(1/5)
BssSI 3496 CTCGTG CACGAG(-5/-1)
DraIII 498 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1374 GACTGTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3469 TGGCCA [CT]^GGCC[GA]
EcoT22I 1569 ATGCAT ATGCA^T
MamI 1706 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3469 TGGCCA TGG^CCA
MscI 3469 TGGCCA TGG^CCA
MspA1I 544 CAGCTG C[AC]G^C[GT]G
NcoI 2945 CCATGG C^CATGG
NheI 2430 GCTAGC G^CTAGC
NsiI 1569 ATGCAT ATGCA^T
PflFI 1044 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2216 ATTAAT AT^TAAT
Psp1406I 3785 AACGTT AA^CGTT
PvuII 544 CAGCTG CAG^CTG
SalI 759 GTCGAC G^TCGAC
SmaI 3364 CCCGGG CCC^GGG
SmlI 3291 CTTAAG C^T[CT][GA]AG
SpeI 2387 ACTAGT A^CTAGT
SphI 2874 GCATGC GCATG^C
SwaI 2455 ATTTAAAT ATTT^AAAT
Tth111I 1044 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2978 TCTAGA T^CTAGA
XmaCI 3364 CCCGGG C^CCGGG
XmaI 3364 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 2781 GACTTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2942 GACCCATGGTC
AocI 201 CCTGAGG CC^T[ACGT]AGG
3860 CCTGAGG
AspEI 2781 GACTTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2942 GACCCATGGTC
BanI 3407 GGTGCC G^G[CT][GA]CC
3910 GGTGCC
BanII 992 GAGCCC G[GA]GC[CT]^C
3545 GAGCCC
BcgI 2803 CGATTTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1062 GCAAGCAACTCG
BpmI 3320 CTGGAG CTGGAG(16/14)
4137 CTGGAG
BsaI 3213 GAGACC GGTCTC(1/5)
2949 GGTCTC
BsaWI 963 ACCGGA [AT]^CCGG[AT]
2672 TCCGGT
BsmI 1189 GAATGC GAATGC(1/-1)
1736 GAATGC
BspHI 566 TCATGA T^CATGA
2467 TCATGA
BspMI 383 GCAGGT ACCTGC(4/8)
597 GCAGGT
BstXI 280 CCAATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3521 CCAAAGTGCTGG
Bsu36I 201 CCTGAGG CC^T[ACGT]AGG
3860 CCTGAGG
CfoI 3552 GCGC GCG^C
3944 GCGC
Eam1105I 2781 GACTTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2942 GACCCATGGTC
Eco81I 201 CCTGAGG CC^T[ACGT]AGG
3860 CCTGAGG
EcoRI 570 GAATTC G^AATTC
2374 GAATTC
FauI 996 CCCGC CCCGC(4/6)
3507 CCCGC
HaeII 3551 AGCGCC [GA]GCGC^[CT]
3943 AGCGCC
HhaI 3552 GCGC GCG^C
3944 GCGC
HinP1I 3552 GCGC G^CGC
3944 GCGC
HindIII 3012 AAGCTT A^AGCTT
4128 AAGCTT
PpuMI 1133 AGGACCT [GA]G^G[AT]CC[CT]
3932 AGGTCCC
RcaI 566 TCATGA T^CATGA
2467 TCATGA
ScaI 1977 AGTACT AGT^ACT
2225 AGTACT
SspI 1552 AATATT AAT^ATT
2452 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BbsI
BbvI BclI BcnI BfaI BmyI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII