################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AviII AvrII BbeI BbrPI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PstI PvuI RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SspBI StuI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AclI 3785 AACGTT AA^CGTT AflII 3291 CTTAAG C^TTAAG AflIII 41 ACGTGT A^C[GA][CT]GT AlwNI 3550 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 2216 ATTAAT AT^TAAT AsnI 2216 ATTAAT AT^TAAT AspHI 67 GTGCTC G[AT]GC[AT]^C AspI 1044 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 3469 TGGCCA TGG^CCA BamHI 104 GGATCC G^GATCC BbvCI 3387 CCTCAGC CCTCAGC(-5/-2) BciVI 3856 GTATCC GTATCC(6/5) BfrI 3291 CTTAAG C^TTAAG BsaBI 1706 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 381 GTGCAG GTGCAG(16/14) BsiHKAI 67 GTGCTC G[AT]GC[AT]^C BsmBI 3449 GAGACG CGTCTC(1/5) BssSI 3496 CTCGTG CACGAG(-5/-1) DraIII 498 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1374 GACTGTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 3469 TGGCCA [CT]^GGCC[GA] EcoT22I 1569 ATGCAT ATGCA^T MamI 1706 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 3469 TGGCCA TGG^CCA MscI 3469 TGGCCA TGG^CCA MspA1I 544 CAGCTG C[AC]G^C[GT]G NcoI 2945 CCATGG C^CATGG NheI 2430 GCTAGC G^CTAGC NsiI 1569 ATGCAT ATGCA^T PflFI 1044 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 2216 ATTAAT AT^TAAT Psp1406I 3785 AACGTT AA^CGTT PvuII 544 CAGCTG CAG^CTG SalI 759 GTCGAC G^TCGAC SmaI 3364 CCCGGG CCC^GGG SmlI 3291 CTTAAG C^T[CT][GA]AG SpeI 2387 ACTAGT A^CTAGT SphI 2874 GCATGC GCATG^C SwaI 2455 ATTTAAAT ATTT^AAAT Tth111I 1044 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2978 TCTAGA T^CTAGA XmaCI 3364 CCCGGG C^CCGGG XmaI 3364 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AhdI 2781 GACTTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2942 GACCCATGGTC AocI 201 CCTGAGG CC^T[ACGT]AGG 3860 CCTGAGG AspEI 2781 GACTTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2942 GACCCATGGTC BanI 3407 GGTGCC G^G[CT][GA]CC 3910 GGTGCC BanII 992 GAGCCC G[GA]GC[CT]^C 3545 GAGCCC BcgI 2803 CGATTTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1062 GCAAGCAACTCG BpmI 3320 CTGGAG CTGGAG(16/14) 4137 CTGGAG BsaI 3213 GAGACC GGTCTC(1/5) 2949 GGTCTC BsaWI 963 ACCGGA [AT]^CCGG[AT] 2672 TCCGGT BsmI 1189 GAATGC GAATGC(1/-1) 1736 GAATGC BspHI 566 TCATGA T^CATGA 2467 TCATGA BspMI 383 GCAGGT ACCTGC(4/8) 597 GCAGGT BstXI 280 CCAATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3521 CCAAAGTGCTGG Bsu36I 201 CCTGAGG CC^T[ACGT]AGG 3860 CCTGAGG CfoI 3552 GCGC GCG^C 3944 GCGC Eam1105I 2781 GACTTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2942 GACCCATGGTC Eco81I 201 CCTGAGG CC^T[ACGT]AGG 3860 CCTGAGG EcoRI 570 GAATTC G^AATTC 2374 GAATTC FauI 996 CCCGC CCCGC(4/6) 3507 CCCGC HaeII 3551 AGCGCC [GA]GCGC^[CT] 3943 AGCGCC HhaI 3552 GCGC GCG^C 3944 GCGC HinP1I 3552 GCGC G^CGC 3944 GCGC HindIII 3012 AAGCTT A^AGCTT 4128 AAGCTT PpuMI 1133 AGGACCT [GA]G^G[AT]CC[CT] 3932 AGGTCCC RcaI 566 TCATGA T^CATGA 2467 TCATGA ScaI 1977 AGTACT AGT^ACT 2225 AGTACT SspI 1552 AATATT AAT^ATT 2452 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI AvaI AvaII BbsI BbvI BclI BcnI BfaI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII