##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Aor51HI AscI Asp718I AvaI AviII
BamHI BbeI BbrPI BbvCI BfrI
BglI BsmBI BsoBI BssSI Bst1107I
BstZ17I CpoI EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
FspI HindIII KasI KpnI KspI
MluI NarI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SmaI SnaBI SpeI Sse8387I SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 4524 GTCTAC GT^[AC][GT]AC
AhdI 1170 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2304 GTGCAC G^TGCAC
AocI 3003 CCTCAGG CC^T[ACGT]AGG
ApaLI 2304 GTGCAC G^TGCAC
AspEI 1170 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2216 CCTAGG C^CTAGG
BciVI 3489 GTATCC GTATCC(6/5)
BclI 1688 TGATCA T^GATCA
BglII 4059 AGATCT A^GATCT
BlnI 2216 CCTAGG C^CTAGG
BlpI 2027 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2027 GCTGAGC GC^T[ACGT]AGC
BsaAI 3140 CACGTA [CT]AC^GT[GA]
BsaBI 322 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3913 CGGTCG CG[GA][CT]^CG
BsiWI 1583 CGTACG C^GTACG
BsmI 4026 GAATGC GAATGC(1/-1)
BspDI 948 ATCGAT AT^CGAT
BsrFI 5 GCCGGC [GA]^CCGG[CT]
BssHII 3590 GCGCGC G^CGCGC
BstBI 1988 TTCGAA TT^CGAA
BstEII 3368 GGTGACC G^GT[ACGT]ACC
BstPI 3368 GGTGACC G^GT[ACGT]ACC
BstYI 4059 AGATCT [GA]^GATC[CT]
Bsu36I 3003 CCTCAGG CC^T[ACGT]AGG
CelII 2027 GCTGAGC GC^T[ACGT]AGC
Cfr10I 5 GCCGGC [GA]^CCGG[CT]
ClaI 948 ATCGAT AT^CGAT
DraI 4300 TTTAAA TTT^AAA
DraIII 3159 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1887 GACAGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1170 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3003 CCTCAGG CC^T[ACGT]AGG
EcoNI 3874 CCTCTCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3368 GGTGACC G^GT[ACGT]ACC
FbaI 1688 TGATCA T^GATCA
HaeII 4009 AGCGCC [GA]GCGC^[CT]
HpaI 3102 GTTAAC GTT^AAC
MamI 322 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 4059 AGATCT [GA]^GATC[CT]
NaeI 5 GCCGGC GCC^GGC
NdeI 136 CATATG CA^TATG
NgoMIV 5 GCCGGC G^CCGGC
NspV 1988 TTCGAA TT^CGAA
PpuMI 2212 GGGTCCT [GA]G^G[AT]CC[CT]
SapI 3825 GAAGAGC GCTCTTC(1/4)
ScaI 1213 AGTACT AGT^ACT
SfuI 1988 TTCGAA TT^CGAA
SgrAI 4 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2684 GCATGC GCATG^C
XhoII 4059 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 1243 TCCGGA T^CCGGA
2984 TCCGGA
ApaI 1267 GGGCCC GGGCC^C
1771 GGGCCC
Asp700I 2172 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3852 GAACGGCTTC
AspI 382 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1008 GACCCTGTC
BalI 3116 TGGCCA TGG^CCA
3724 TGGCCA
BcgI 2014 GCATCTTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3124 GCACCAGGGTCG
BseAI 1243 TCCGGA T^CCGGA
2984 TCCGGA
BseRI 63 CTCCTC GAGGAG(10/8)
799 CTCCTC
Bsp1407I 502 TGTACA T^GTACA
4334 TGTACA
BspEI 1243 TCCGGA T^CCGGA
2984 TCCGGA
BspHI 3019 TCATGA T^CATGA
3148 TCATGA
BsrBI 40 CCGCTC CCGCTC(-3/-3)
4165 CCGCTC
BsrGI 502 TGTACA T^GTACA
4334 TGTACA
BstAPI 891 GCATTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2767 GCATGGGCTGC
MfeI 155 CAATTG C^AATTG
2542 CAATTG
MluNI 3116 TGGCCA TGG^CCA
3724 TGGCCA
MroI 1243 TCCGGA T^CCGGA
2984 TCCGGA
MscI 3116 TGGCCA TGG^CCA
3724 TGGCCA
MunI 155 CAATTG C^AATTG
2542 CAATTG
PflFI 382 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1008 GACCCTGTC
PshAI 1626 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3371 GACCATGGTC
RcaI 3019 TCATGA T^CATGA
3148 TCATGA
SacI 2030 GAGCTC GAGCT^C
3393 GAGCTC
SexAI 1357 ACCAGGT A^CC[AT]GGT
3502 ACCTGGT
SmlI 533 CTCAAG C^T[CT][GA]AG
1037 CTTGAG
SspBI 502 TGTACA T^GTACA
4334 TGTACA
Tth111I 382 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1008 GACCCTGTC
XmnI 2172 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3852 GAACGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI