##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccIII AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AviII AvrII BamHI BbrPI BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstZ17I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoT22I FseI
FspI HpaI KpnI KspI MamI
MluI MroI NotI NruI NsiI
PacI PmaCI PmeI PmlI PshAI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SnaBI SphI Sse8387I
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AclI 5208 AACGTT AA^CGTT
AgeI 4932 ACCGGT A^CCGGT
AocI 6121 CCTTAGG CC^T[ACGT]AGG
ApaI 3455 GGGCCC GGGCC^C
AspI 229 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 58 GGCGCC GGCGC^C
BbvCI 279 CCTCAGC CCTCAGC(-5/-2)
BsmBI 103 GAGACG CGTCTC(1/5)
Bsp1407I 6485 TGTACA T^GTACA
BsrDI 2802 GCAATG GCAATG(2/0)
BsrGI 6485 TGTACA T^GTACA
BstEII 722 GGTCACC G^GT[ACGT]ACC
BstPI 722 GGTCACC G^GT[ACGT]ACC
Bsu36I 6121 CCTTAGG CC^T[ACGT]AGG
Eco81I 6121 CCTTAGG CC^T[ACGT]AGG
EcoO65I 722 GGTCACC G^GT[ACGT]ACC
KasI 58 GGCGCC G^GCGCC
NarI 58 GGCGCC GG^CGCC
NspI 2052 GCATGT [GA]CATG^[CT]
PaeR7I 1752 CTCGAG C^TCGAG
PflFI 229 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4932 ACCGGT A^CCGGT
Psp1406I 5208 AACGTT AA^CGTT
SapI 4706 GAAGAGC GCTCTTC(1/4)
SfoI 58 GGCGCC GGC^GCC
SgrAI 107 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 179 CCCGGG CCC^GGG
SpeI 4394 ACTAGT A^CTAGT
SspBI 6485 TGTACA T^GTACA
SwaI 3444 ATTTAAAT ATTT^AAAT
Tth111I 229 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1752 CTCGAG C^TCGAG
XmaCI 179 CCCGGG C^CCGGG
XmaI 179 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 3373 GTCTAC GT^[AC][GT]AC
7572 GTCTAC
AflII 402 CTTAAG C^TTAAG
3736 CTTAAG
AflIII 3493 ACGTGT A^C[GA][CT]GT
5903 ACGTGT
BalI 5183 TGGCCA TGG^CCA
6699 TGGCCA
BcgI 286 GCAAAGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4750 GCAGGTGCTTCG
BfrI 402 CTTAAG C^TTAAG
3736 CTTAAG
BglI 201 GCCGGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5508 GCCATAAAGGC
BglII 2741 AGATCT A^GATCT
3474 AGATCT
BlpI 114 GCTGAGC GC^T[ACGT]AGC
3925 GCTCAGC
Bpu1102I 114 GCTGAGC GC^T[ACGT]AGC
3925 GCTCAGC
BsmFI 36 GGGAC GGGAC(10/14)
4387 GGGAC
BstAPI 1655 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3133 GCAAAGTCTGC
BstXI 640 CCATGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5060 CCAACAAGCTGG
CelII 114 GCTGAGC GC^T[ACGT]AGC
3925 GCTCAGC
EcoNI 186 CCTCGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4661 CCTACAAAAGG
EcoRI 1537 GAATTC G^AATTC
7427 GAATTC
EcoRV 2334 GATATC GAT^ATC
4597 GATATC
HindIII 399 AAGCTT A^AGCTT
4072 AAGCTT
MfeI 461 CAATTG C^AATTG
3876 CAATTG
MluNI 5183 TGGCCA TGG^CCA
6699 TGGCCA
MscI 5183 TGGCCA TGG^CCA
6699 TGGCCA
MunI 461 CAATTG C^AATTG
3876 CAATTG
NaeI 29 GCCGGC GCC^GGC
108 GCCGGC
NdeI 2891 CATATG CA^TATG
6092 CATATG
NgoMIV 29 GCCGGC G^CCGGC
108 GCCGGC
NheI 2789 GCTAGC G^CTAGC
3115 GCTAGC
PvuII 3958 CAGCTG CAG^CTG
5190 CAGCTG
SacI 4169 GAGCTC GAGCT^C
6431 GAGCTC
SexAI 4794 ACCTGGT A^CC[AT]GGT
6752 ACCTGGT
SfiI 200 GGCCGGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5507 GGCCATAAAGGCC
StuI 52 AGGCCT AGG^CCT
5515 AGGCCT
XbaI 3034 TCTAGA T^CTAGA
7402 TCTAGA
XcmI 2675 CCAGATCCTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6713 CCAGGGGCTAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvI BciVI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstBI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspV
PflMI PleI PpuMI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfuI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI