##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BcnI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BsrBI BssHII BssSI BstBI BstUI
CelII ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I FauI
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MvnI NarI NciI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SphI Sse8387I
StuI SwaI XbaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 3371 CTTAAG C^TTAAG
AflIII 1545 ACATGT A^C[GA][CT]GT
AhdI 1082 GACTTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2313 CCTTAGG CC^T[ACGT]AGG
AspEI 1082 GACTTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 1036 TGATCA T^GATCA
BfrI 3371 CTTAAG C^TTAAG
BglI 316 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 4172 CTCCTC GAGGAG(10/8)
BsmI 3442 GCATTC GAATGC(1/-1)
BspLU11I 1545 ACATGT A^CATGT
BsrFI 749 GCCGGC [GA]^CCGG[CT]
BstAPI 753 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4268 GGTTACC G^GT[ACGT]ACC
BstPI 4268 GGTTACC G^GT[ACGT]ACC
Bsu36I 2313 CCTTAGG CC^T[ACGT]AGG
CfoI 675 GCGC GCG^C
Cfr10I 749 GCCGGC [GA]^CCGG[CT]
DsaI 541 CCATGG C^C[GA][CT]GG
EaeI 703 CGGCCA [CT]^GGCC[GA]
Eam1105I 1082 GACTTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2313 CCTTAGG CC^T[ACGT]AGG
EcoNI 3204 CCTTTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4268 GGTTACC G^GT[ACGT]ACC
EcoRI 1852 GAATTC G^AATTC
FbaI 1036 TGATCA T^GATCA
HapII 750 CCGG C^CGG
HhaI 675 GCGC GCG^C
HinP1I 675 GCGC G^CGC
HpaII 750 CCGG C^CGG
MfeI 2074 CAATTG C^AATTG
MspA1I 318 CAGCTG C[AC]G^C[GT]G
MspI 750 CCGG C^CGG
MunI 2074 CAATTG C^AATTG
NaeI 749 GCCGGC GCC^GGC
NcoI 541 CCATGG C^CATGG
NdeI 1530 CATATG CA^TATG
NgoMIV 749 GCCGGC G^CCGGC
NheI 344 GCTAGC G^CTAGC
PflMI 150 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 318 CAGCTG CAG^CTG
SacI 4495 GAGCTC GAGCT^C
SapI 4180 GAAGAGC GCTCTTC(1/4)
SpeI 3267 ACTAGT A^CTAGT
Van91I 150 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 206 GCGG CCGC(-3/-1)
702 GCGG
AlwNI 3908 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
4540 CAGCCACTG
AspHI 2843 GAGCAC G[AT]GC[AT]^C
4495 GAGCTC
AspI 1168 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4064 GACCAAGTC
BanII 640 GAGCCC G[GA]GC[CT]^C
4495 GAGCTC
BbvCI 1229 CCTCAGC CCTCAGC(-5/-2)
4458 GCTGAGG
BglII 1067 AGATCT A^GATCT
2250 AGATCT
BsaAI 2162 TACGTA [CT]AC^GT[GA]
2939 TACGTA
BsaI 4759 GAGACC GGTCTC(1/5)
1330 GGTCTC
BsgI 225 CTGCAC GTGCAG(16/14)
1199 GTGCAG
BsiHKAI 2843 GAGCAC G[AT]GC[AT]^C
4495 GAGCTC
BsrDI 89 CATTGC GCAATG(2/0)
861 GCAATG
DraII 590 GGGACCT [GA]G^G[ACGT]CC[CT]
3337 GGGACCC
EcoO109I 590 GGGACCT [GA]G^G[ACGT]CC[CT]
3337 GGGACCC
EcoRV 2178 GATATC GAT^ATC
4306 GATATC
EcoT14I 541 CCATGG C^C[AT][AT]GG
4216 CCAAGG
EcoT22I 171 ATGCAT ATGCA^T
1892 ATGCAT
HindIII 3153 AAGCTT A^AGCTT
4410 AAGCTT
NsiI 171 ATGCAT ATGCA^T
1892 ATGCAT
PaeR7I 1214 CTCGAG C^TCGAG
4175 CTCGAG
PflFI 1168 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4064 GACCAAGTC
PleI 1172 GAGTC GAGTC(4/5)
4728 GAGTC
PpuMI 590 GGGACCT [GA]G^G[AT]CC[CT]
3337 GGGACCC
SnaBI 2162 TACGTA TAC^GTA
2939 TACGTA
StyI 541 CCATGG C^C[AT][AT]GG
4216 CCAAGG
Tth111I 1168 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4064 GACCAAGTC
XhoI 1214 CTCGAG C^TCGAG
4175 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AvaI
AvaII BbsI BbvI BciVI BfaI
BmyI BpmI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
Bst1107I BstF5I BstNI BstXI BstYI
BstZ17I Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI