##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AviII AvrII
BbeI BbrPI BcgI BlnI BsaBI
BsaWI BseAI BsiEI BspDI BspEI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV FseI FspI HincII HindII
HpaI KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SwaI
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AclI 1388 AACGTT AA^CGTT
AcyI 380 GGCGTC G[GA]^CG[CT]C
Alw44I 3056 GTGCAC G^TGCAC
AlwNI 1218 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 3056 GTGCAC G^TGCAC
AspHI 3056 GTGCAC G[AT]GC[AT]^C
AvaI 3408 CCCGAG C^[CT]CG[GA]G
BalI 3491 TGGCCA TGG^CCA
BanII 4743 GGGCTC G[GA]GC[CT]^C
BciVI 3661 GGATAC GTATCC(6/5)
BglII 2096 AGATCT A^GATCT
BlpI 1464 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1464 GCTCAGC GC^T[ACGT]AGC
BsaAI 14 TACGTG [CT]AC^GT[GA]
BsaHI 380 GGCGTC G[GA]^CG[CT]C
BseRI 1396 GAGGAG GAGGAG(10/8)
BsiHKAI 3056 GTGCAC G[AT]GC[AT]^C
BsiWI 12 CGTACG C^GTACG
BsoBI 3408 CCCGAG C^[CT]CG[GA]G
Bsp1407I 4374 TGTACA T^GTACA
BspHI 3866 TCATGA T^CATGA
BspLU11I 2710 ACATGT A^CATGT
BsrGI 4374 TGTACA T^GTACA
BssSI 2558 CACGAG CACGAG(-5/-1)
BstAPI 1588 GCATGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1464 GCTCAGC GC^T[ACGT]AGC
EcoT22I 957 ATGCAT ATGCA^T
FauI 1634 GCGGG CCCGC(4/6)
HaeII 4313 AGCGCC [GA]GCGC^[CT]
HgaI 381 GCGTC GACGC(5/10)
Hin1I 380 GGCGTC G[GA]^CG[CT]C
MfeI 1963 CAATTG C^AATTG
MluNI 3491 TGGCCA TGG^CCA
MscI 3491 TGGCCA TGG^CCA
MunI 1963 CAATTG C^AATTG
NsiI 957 ATGCAT ATGCA^T
Psp1406I 1388 AACGTT AA^CGTT
RcaI 3866 TCATGA T^CATGA
SpeI 2836 ACTAGT A^CTAGT
SphI 955 GCATGC GCATG^C
SspBI 4374 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflII 2584 CTTAAG C^TTAAG
2697 CTTAAG
BanI 2531 GGTGCC G^G[CT][GA]CC
3428 GGCACC
BbsI 425 GAAGAC GAAGAC(2/6)
1407 GAAGAC
BbvCI 4763 CCTCAGC CCTCAGC(-5/-2)
5226 GCTGAGG
BclI 4283 TGATCA T^GATCA
4484 TGATCA
BcnI 1119 CCGGG CC^[GC]GG
3578 CCCGG
BfrI 2584 CTTAAG C^TTAAG
2697 CTTAAG
BglI 1665 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2428 GCCTAGAGGGC
BpuAI 425 GAAGAC GAAGAC(2/6)
1407 GAAGAC
BsmBI 3471 GAGACG CGTCTC(1/5)
4158 GAGACG
BstXI 3543 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5211 CCAGCTACTTGG
CfoI 1597 GCGC GCG^C
4314 GCGC
EaeI 3491 TGGCCA [CT]^GGCC[GA]
5041 CGGCCA
EarI 1186 CTCTTC CTCTTC(1/4)
4247 CTCTTC
EcoRI 122 GAATTC G^AATTC
1556 GAATTC
FbaI 4283 TGATCA T^GATCA
4484 TGATCA
HapII 1119 CCGG C^CGG
3579 CCGG
HhaI 1597 GCGC GCG^C
4314 GCGC
HinP1I 1597 GCGC G^CGC
4314 GCGC
HpaII 1119 CCGG C^CGG
3579 CCGG
Ksp632I 1186 CTCTTC CTCTTC(1/4)
4247 CTCTTC
MspA1I 2382 CAGCTG C[AC]G^C[GT]G
3414 CAGCTG
MspI 1119 CCGG C^CGG
3579 CCGG
NciI 1119 CCGGG CC^[GC]GG
3578 CCCGG
NcoI 396 CCATGG C^CATGG
3809 CCATGG
NdeI 3236 CATATG CA^TATG
4498 CATATG
PvuII 2382 CAGCTG CAG^CTG
3414 CAGCTG
ScaI 990 AGTACT AGT^ACT
3962 AGTACT
StuI 2426 AGGCCT AGG^CCT
3244 AGGCCT
TaqI 3505 TCGA T^CGA
4888 TCGA
TthHB8I 3505 TCGA T^CGA
4888 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspI AvaII BamHI BbvI BfaI
BmyI BpmI BsaI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DsaI EcoRII
EcoT14I Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NheI NlaIII NlaIV NspI PflFI
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I XhoII XmnI