##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
Asp700I AspEI AspI AvrII BbeI
BbrPI BclI BfrI BglII BlnI
BsmBI BspHI BssHII BssSI BstAPI
BstBI BstEII BstPI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoT22I FbaI FseI HpaI
KasI KspI MfeI MluI MunI
NarI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1855 GACGTC GACGT^C
AccII 3093 CGCG CG^CG
Alw44I 2268 GTGCAC G^TGCAC
AlwNI 3030 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1845 CCTGAGG CC^T[ACGT]AGG
Aor51HI 831 AGCGCT AGC^GCT
ApaLI 2268 GTGCAC G^TGCAC
AvaI 1322 CCCGGG C^[CT]CG[GA]G
AviII 2104 TGCGCA TGC^GCA
BbvCI 3826 GCTGAGG CCTCAGC(-5/-2)
BcgI 1066 CGATGTACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 3682 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3682 GCTCAGC GC^T[ACGT]AGC
BsaI 2429 GAGACC GGTCTC(1/5)
BsiEI 3484 CGACCG CG[GA][CT]^CG
BsiWI 2680 CGTACG C^GTACG
BsoBI 1322 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3082 TGTACA T^GTACA
BspDI 1064 ATCGAT AT^CGAT
BsrDI 4205 CATTGC GCAATG(2/0)
BsrGI 3082 TGTACA T^GTACA
BstUI 3093 CGCG CG^CG
BstXI 3449 CCATGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1845 CCTGAGG CC^T[ACGT]AGG
CelII 3682 GCTCAGC GC^T[ACGT]AGC
ClaI 1064 ATCGAT AT^CGAT
Eco47III 831 AGCGCT AGC^GCT
Eco81I 1845 CCTGAGG CC^T[ACGT]AGG
EcoRI 2004 GAATTC G^AATTC
FspI 2104 TGCGCA TGC^GCA
HaeII 831 AGCGCT [GA]GCGC^[CT]
MvnI 3093 CGCG CG^CG
NheI 4777 GCTAGC G^CTAGC
SapI 1294 GAAGAGC GCTCTTC(1/4)
ScaI 4721 AGTACT AGT^ACT
SfiI 634 GGCCCAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1322 CCCGGG CCC^GGG
SphI 3638 GCATGC GCATG^C
SspBI 3082 TGTACA T^GTACA
SwaI 4460 ATTTAAAT ATTT^AAAT
XcmI 801 CCAGGAAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1322 CCCGGG C^CCGGG
XmaI 1322 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 2175 TCCGGA T^CCGGA
2526 TCCGGA
ApaI 1150 GGGCCC GGGCC^C
1251 GGGCCC
AspHI 2268 GTGCAC G[AT]GC[AT]^C
2351 GTGCTC
BamHI 1095 GGATCC G^GATCC
4794 GGATCC
BglI 635 GCCCAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2153 GCCACAAAGGC
BsaBI 2974 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3907 GATGGGTATC
BseAI 2175 TCCGGA T^CCGGA
2526 TCCGGA
BsiHKAI 2268 GTGCAC G[AT]GC[AT]^C
2351 GTGCTC
BspEI 2175 TCCGGA T^CCGGA
2526 TCCGGA
BspLU11I 2141 ACATGT A^CATGT
2557 ACATGT
BsrBI 145 CCGCTC CCGCTC(-3/-3)
2943 CCGCTC
EcoRV 1606 GATATC GAT^ATC
2443 GATATC
MamI 2974 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3907 GATGGGTATC
MroI 2175 TCCGGA T^CCGGA
2526 TCCGGA
NaeI 3572 GCCGGC GCC^GGC
3713 GCCGGC
NdeI 3722 CATATG CA^TATG
4352 CATATG
NgoMIV 3572 GCCGGC G^CCGGC
3713 GCCGGC
PpuMI 2506 GGGTCCT [GA]G^G[AT]CC[CT]
2881 GGGTCCT
SspI 4477 AATATT AAT^ATT
4580 AATATT
StuI 1244 AGGCCT AGG^CCT
3629 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
Acc65I AccI AciI AcsI AcyI
AfaI AflIII AluI AlwI ApoI
AseI AsnI Asp718I AvaII BalI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrFI BsrI
BssKI Bst1107I BstF5I BstNI BstYI
BstZ17I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PshAI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII