##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AspI AviII BbeI BcgI
BciVI BsgI BsiEI BsiWI BsmBI
BspDI BsrFI BstAPI BstBI Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI HaeII KasI KpnI MluI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI Psp1406I
PstI PvuI RsrII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SpeI SphI Sse8387I Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 2950 GTATAC GT^[AC][GT]AC
AccIII 4253 TCCGGA T^CCGGA
AcyI 3 GGCGTC G[GA]^CG[CT]C
AflII 3771 CTTAAG C^TTAAG
Alw44I 3051 GTGCAC G^TGCAC
AlwNI 4771 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 3051 GTGCAC G^TGCAC
AvrII 1121 CCTAGG C^CTAGG
BamHI 2835 GGATCC G^GATCC
BanI 2817 GGTGCC G^G[CT][GA]CC
BbvCI 3686 GCTGAGG CCTCAGC(-5/-2)
BclI 4839 TGATCA T^GATCA
BfrI 3771 CTTAAG C^TTAAG
BglII 3767 AGATCT A^GATCT
BlnI 1121 CCTAGG C^CTAGG
BsaBI 3571 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaWI 4253 TCCGGA [AT]^CCGG[AT]
BseAI 4253 TCCGGA T^CCGGA
Bsp1407I 4146 TGTACA T^GTACA
BspEI 4253 TCCGGA T^CCGGA
BspLU11I 3047 ACATGT A^CATGT
BspMI 2662 GCAGGT ACCTGC(4/8)
BsrBI 1860 CCGCTC CCGCTC(-3/-3)
BsrGI 4146 TGTACA T^GTACA
BssHII 50 GCGCGC G^CGCGC
BssSI 1310 CACGAG CACGAG(-5/-1)
Bst1107I 2950 GTATAC GTA^TAC
BstEII 1884 GGTGACC G^GT[ACGT]ACC
BstPI 1884 GGTGACC G^GT[ACGT]ACC
BstZ17I 2950 GTATAC GTA^TAC
EcoNI 2656 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1884 GGTGACC G^GT[ACGT]ACC
FauI 4638 GCGGG CCCGC(4/6)
FbaI 4839 TGATCA T^GATCA
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HindIII 2415 AAGCTT A^AGCTT
MamI 3571 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4065 CAATTG C^AATTG
MroI 4253 TCCGGA T^CCGGA
MunI 4065 CAATTG C^AATTG
NcoI 4347 CCATGG C^CATGG
PshAI 1417 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1373 CAGCTG CAG^CTG
SacI 320 GAGCTC GAGCT^C
SapI 1862 GCTCTTC GCTCTTC(1/4)
SfaNI 3571 GATGC GCATC(5/9)
SnaBI 1095 TACGTA TAC^GTA
SspBI 4146 TGTACA T^GTACA
SspI 3308 AATATT AAT^ATT
SwaI 2569 ATTTAAAT ATTT^AAAT
XcmI 2631 CCACCTGTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 519 CCTCAGG CC^T[ACGT]AGG
2931 CCTGAGG
BanII 320 GAGCTC G[GA]GC[CT]^C
3595 GAGCCC
BbrPI 2267 CACGTG CAC^GTG
2812 CACGTG
BlpI 12 GCTCAGC GC^T[ACGT]AGC
1936 GCTTAGC
Bpu1102I 12 GCTCAGC GC^T[ACGT]AGC
1936 GCTTAGC
Bsu36I 519 CCTCAGG CC^T[ACGT]AGG
2931 CCTGAGG
CelII 12 GCTCAGC GC^T[ACGT]AGC
1936 GCTTAGC
DraIII 2766 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
2812 CACGTGGTG
DrdI 2676 GACTCAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3281 GACTTAGGTGTC
Eco81I 519 CCTCAGG CC^T[ACGT]AGG
2931 CCTGAGG
KspI 710 CCGCGG CCGC^GG
4633 CCGCGG
PflMI 1266 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4770 CCAGGTTCTGG
PmaCI 2267 CACGTG CAC^GTG
2812 CACGTG
PmlI 2267 CACGTG CAC^GTG
2812 CACGTG
PpuMI 1586 AGGACCC [GA]G^G[AT]CC[CT]
4342 AGGACCC
SacII 710 CCGCGG CCGC^GG
4633 CCGCGG
SexAI 3100 ACCAGGT A^CC[AT]GGT
4549 ACCAGGT
SfcI 452 CTACAG C^T[GA][CT]AG
1293 CTATAG
SmaI 126 CCCGGG CCC^GGG
1890 CCCGGG
SmlI 3757 CTCAAG C^T[CT][GA]AG
3771 CTTAAG
StuI 2735 AGGCCT AGG^CCT
4045 AGGCCT
TaqI 294 TCGA T^CGA
4615 TCGA
TthHB8I 294 TCGA T^CGA
4615 TCGA
Van91I 1266 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4770 CCAGGTTCTGG
XmaCI 126 CCCGGG C^CCGGG
1890 CCCGGG
XmaI 126 CCCGGG C^CCGGG
1890 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AspHI AvaI AvaII BalI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI RcaI
RsaI Sau3AI Sau96I ScrFI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII