##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AgeI AhdI ApaI AscI
AspEI AvaI AvrII BbrPI BcgI
BglII BlnI BsaAI BsaBI BsaI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BsrGI BssHII BstBI
BstEII BstPI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
HpaI MamI MfeI MroI MunI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PvuI RsrII SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 3754 GGTACC G^GTACC
AclI 3693 AACGTT AA^CGTT
AflII 3056 CTTAAG C^TTAAG
Alw44I 1525 GTGCAC G^TGCAC
Aor51HI 30 AGCGCT AGC^GCT
ApaLI 1525 GTGCAC G^TGCAC
Asp718I 3754 GGTACC G^GTACC
AviII 120 TGCGCA TGC^GCA
BbeI 55 GGCGCC GGCGC^C
BbvCI 3133 CCTCAGC CCTCAGC(-5/-2)
BciVI 4750 GTATCC GTATCC(6/5)
BfrI 3056 CTTAAG C^TTAAG
BsaWI 1419 ACCGGA [AT]^CCGG[AT]
BseRI 1722 CTCCTC GAGGAG(10/8)
BstAPI 3268 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III 30 AGCGCT AGC^GCT
EcoRI 4126 GAATTC G^AATTC
FseI 2308 GGCCGGCC GGCCGG^CC
FspI 120 TGCGCA TGC^GCA
KasI 55 GGCGCC G^GCGCC
KpnI 3754 GGTACC GGTAC^C
KspI 103 CCGCGG CCGC^GG
MluI 115 ACGCGT A^CGCGT
NarI 55 GGCGCC GG^CGCC
PshAI 2162 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3693 AACGTT AA^CGTT
SacI 3713 GAGCTC GAGCT^C
SacII 103 CCGCGG CCGC^GG
ScaI 3153 AGTACT AGT^ACT
SexAI 4807 ACCAGGT A^CC[AT]GGT
SfoI 55 GGCGCC GGC^GCC
SphI 4989 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 557 GACGTC GACGT^C
1820 GACGTC
AseI 234 ATTAAT AT^TAAT
4580 ATTAAT
AsnI 234 ATTAAT AT^TAAT
4580 ATTAAT
BcnI 1857 CCCGG CC^[GC]GG
4138 CCCGG
BglI 3295 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3728 GCCCAGCAGGC
BsgI 2679 CTGCAC GTGCAG(16/14)
3962 GTGCAG
BsmBI 112 GAGACG CGTCTC(1/5)
555 GAGACG
BspHI 4573 TCATGA T^CATGA
4589 TCATGA
BspLU11I 3285 ACATGT A^CATGT
3356 ACATGT
BspMI 2261 ACCTGC ACCTGC(4/8)
3737 GCAGGT
BsrBI 94 GAGCGG CCGCTC(-3/-3)
3887 GAGCGG
BstXI 3483 CCATGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4097 CCAGAAATTTGG
DraIII 1550 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3948 CACTGAGTG
EcoRV 3245 GATATC GAT^ATC
3850 GATATC
HaeII 30 AGCGCT [GA]GCGC^[CT]
55 GGCGCC
NaeI 173 GCCGGC GCC^GGC
2309 GCCGGC
NciI 1857 CCCGG CC^[GC]GG
4138 CCCGG
NgoMIV 173 GCCGGC G^CCGGC
2309 GCCGGC
PshBI 234 ATTAAT AT^TAAT
4580 ATTAAT
RcaI 4573 TCATGA T^CATGA
4589 TCATGA
SapI 1312 GAAGAGC GCTCTTC(1/4)
1904 GAAGAGC
SspI 2922 AATATT AAT^ATT
5156 AATATT
TaqI 2449 TCGA T^CGA
3709 TCGA
TthHB8I 2449 TCGA T^CGA
3709 TCGA
XbaI 1609 TCTAGA T^CTAGA
5178 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI Asp700I AspHI AspI
AvaII BalI BamHI BanI BanII
BbsI BbvI BclI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrFI
BsrI BssKI BssSI Bst1107I BstF5I
BstNI BstUI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NcoI NdeII NlaIII
NlaIV NsiI NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
Van91I XcmI XhoII XmnI