##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AclI AcyI AflIII AgeI
AlwNI Aor51HI ApaI AscI AvaI
AviII AvrII BamHI BbeI BbrPI
BlnI BsaBI BsaHI BsiWI BsoBI
BspDI BspLU11I BssHII BstBI CfoI
ClaI CpoI DraII DraIII Eco47III
EcoO109I FseI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI MamI
MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SwaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 2293 GGTACC G^GTACC
AccIII 2043 TCCGGA T^CCGGA
AhdI 1244 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 447 GTGCAC G^TGCAC
ApaLI 447 GTGCAC G^TGCAC
Asp700I 2096 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2293 GGTACC G^GTACC
AspEI 1244 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1115 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 992 GAGCCC G[GA]GC[CT]^C
BbvCI 2308 GCTGAGG CCTCAGC(-5/-2)
BcgI 961 CGATAAGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4689 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 971 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 971 GCTGAGC GC^T[ACGT]AGC
BsaAI 3286 TACGTA [CT]AC^GT[GA]
BseAI 2043 TCCGGA T^CCGGA
BsiEI 15 CGGCCG CG[GA][CT]^CG
BspEI 2043 TCCGGA T^CCGGA
BspMI 3148 GCAGGT ACCTGC(4/8)
BsrBI 12 GAGCGG CCGCTC(-3/-3)
BsrFI 1859 GCCGGT [GA]^CCGG[CT]
BssSI 1074 CTCGTG CACGAG(-5/-1)
BstEII 5046 GGTAACC G^GT[ACGT]ACC
BstPI 5046 GGTAACC G^GT[ACGT]ACC
CelII 971 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1859 GCCGGT [GA]^CCGG[CT]
DrdI 3464 GACCTGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 33 CCGCGG C^C[GA][CT]GG
EagI 15 CGGCCG C^GGCCG
Eam1105I 1244 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 15 CGGCCG C^GGCCG
Eco52I 15 CGGCCG C^GGCCG
EcoNI 4376 CCTGCACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5046 GGTAACC G^GT[ACGT]ACC
EcoRV 4778 GATATC GAT^ATC
FauI 23 GCGGG CCCGC(4/6)
KpnI 2293 GGTACC GGTAC^C
KspI 33 CCGCGG CCGC^GG
MfeI 2214 CAATTG C^AATTG
MroI 2043 TCCGGA T^CCGGA
MunI 2214 CAATTG C^AATTG
NdeI 302 CATATG CA^TATG
PacI 2261 TTAATTAA TTAAT^TAA
PflFI 1115 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2327 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1273 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1911 CAGCTG CAG^CTG
SacII 33 CCGCGG CCGC^GG
SnaBI 3286 TACGTA TAC^GTA
Tth111I 1115 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2327 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1192 TCTAGA T^CTAGA
XmnI 2096 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 34 CGCG CG^CG
1884 CGCG
AocI 2476 CCTTAGG CC^T[ACGT]AGG
4023 CCTTAGG
AseI 1199 ATTAAT AT^TAAT
3345 ATTAAT
AsnI 1199 ATTAAT AT^TAAT
3345 ATTAAT
AspHI 447 GTGCAC G[AT]GC[AT]^C
3262 GAGCAC
AvaII 1745 GGTCC G^G[AT]CC
1801 GGACC
BciVI 148 GGATAC GTATCC(6/5)
3269 GTATCC
BclI 1431 TGATCA T^GATCA
1986 TGATCA
BseRI 1038 GAGGAG GAGGAG(10/8)
1670 GAGGAG
BsgI 4377 CTGCAC GTGCAG(16/14)
5098 CTGCAC
BsiHKAI 447 GTGCAC G[AT]GC[AT]^C
3262 GAGCAC
BspHI 1785 TCATGA T^CATGA
3192 TCATGA
BsrDI 2077 CATTGC GCAATG(2/0)
4538 GCAATG
BstUI 34 CGCG CG^CG
1884 CGCG
Bsu36I 2476 CCTTAGG CC^T[ACGT]AGG
4023 CCTTAGG
DraI 3032 TTTAAA TTT^AAA
3647 TTTAAA
Eco81I 2476 CCTTAGG CC^T[ACGT]AGG
4023 CCTTAGG
EcoT14I 1317 CCAAGG C^C[AT][AT]GG
3416 CCTTGG
EcoT22I 771 ATGCAT ATGCA^T
1259 ATGCAT
FbaI 1431 TGATCA T^GATCA
1986 TGATCA
HgaI 1774 GCGTC GACGC(5/10)
2660 GCGTC
HindIII 1519 AAGCTT A^AGCTT
2558 AAGCTT
MspA1I 33 CCGCGG C[AC]G^C[GT]G
1911 CAGCTG
MvnI 34 CGCG CG^CG
1884 CGCG
NsiI 771 ATGCAT ATGCA^T
1259 ATGCAT
NspI 313 GCATGT [GA]CATG^[CT]
2337 GCATGT
PshBI 1199 ATTAAT AT^TAAT
3345 ATTAAT
RcaI 1785 TCATGA T^CATGA
3192 TCATGA
StuI 1461 AGGCCT AGG^CCT
4373 AGGCCT
StyI 1317 CCAAGG C^C[AT][AT]GG
3416 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI BalI BanI
BbsI BbvI BcnI BfaI BfrI
BglII BmyI BpmI BpuAI BsaI
BsaJI BsaWI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BsrGI BsrI BssKI Bst1107I BstAPI
BstF5I BstNI BstXI BstYI BstZ17I
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRI EcoRII Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII