##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BbrPI BciVI BfrI BlnI
BlpI Bpu1102I BsaBI BsaHI BsiWI
BsmBI BspDI BsrFI BssHII Bst1107I
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FseI FspI Hin1I KasI KspI
MamI MluI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI PvuI RsrII
SacII SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2342 GGTACC G^GTACC
AccII 105 CGCG CG^CG
AccIII 4567 TCCGGA T^CCGGA
AclI 4616 AACGTT AA^CGTT
AocI 4199 CCTTAGG CC^T[ACGT]AGG
Asp700I 4550 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2342 GGTACC G^GTACC
BsaWI 4567 TCCGGA [AT]^CCGG[AT]
BseAI 4567 TCCGGA T^CCGGA
BsiEI 532 CGGTCG CG[GA][CT]^CG
BspEI 4567 TCCGGA T^CCGGA
BsrBI 95 GAGCGG CCGCTC(-3/-3)
BssSI 3499 CACGAG CACGAG(-5/-1)
BstAPI 5091 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2729 TTCGAA TT^CGAA
BstUI 105 CGCG CG^CG
Bsu36I 4199 CCTTAGG CC^T[ACGT]AGG
DrdI 2623 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4199 CCTTAGG CC^T[ACGT]AGG
HaeII 136 GGCGCT [GA]GCGC^[CT]
HindIII 1751 AAGCTT A^AGCTT
HpaI 4780 GTTAAC GTT^AAC
KpnI 2342 GGTACC GGTAC^C
MroI 4567 TCCGGA T^CCGGA
MvnI 105 CGCG CG^CG
NcoI 3932 CCATGG C^CATGG
NdeI 2792 CATATG CA^TATG
NspV 2729 TTCGAA TT^CGAA
PshAI 1897 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4616 AACGTT AA^CGTT
SacI 3001 GAGCTC GAGCT^C
SalI 3243 GTCGAC G^TCGAC
SfuI 2729 TTCGAA TT^CGAA
SpeI 2902 ACTAGT A^CTAGT
SphI 4270 GCATGC GCATG^C
XmnI 4550 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AlwNI 3334 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
3514 CAGCAGCTG
ApaI 88 GGGCCC GGGCC^C
111 GGGCCC
AspHI 3001 GAGCTC G[AT]GC[AT]^C
3557 GAGCAC
AvaI 2 CTCGGG C^[CT]CG[GA]G
42 CTCGGG
BamHI 552 GGATCC G^GATCC
2681 GGATCC
BanI 2342 GGTACC G^G[CT][GA]CC
2482 GGCACC
BclI 2030 TGATCA T^GATCA
3407 TGATCA
BglI 142 GCCCCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1120 GCCAAAAGGGC
BpmI 10 CTGGAG CTGGAG(16/14)
1775 CTGGAG
BsaAI 2488 TACGTA [CT]AC^GT[GA]
2528 TACGTA
BsaI 172 GAGACC GGTCTC(1/5)
3443 GAGACC
BsiHKAI 3001 GAGCTC G[AT]GC[AT]^C
3557 GAGCAC
BsmFI 101 GGGAC GGGAC(10/14)
3528 GTCCC
BsmI 508 GAATGC GAATGC(1/-1)
4907 GCATTC
BsoBI 2 CTCGGG C^[CT]CG[GA]G
42 CTCGGG
Bsp1407I 1361 TGTACA T^GTACA
1465 TGTACA
BspHI 2363 TCATGA T^CATGA
3249 TCATGA
BsrGI 1361 TGTACA T^GTACA
1465 TGTACA
BstXI 557 CCAAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3716 CCAAAGAAATGG
DraI 3120 TTTAAA TTT^AAA
3150 TTTAAA
EcoNI 1476 CCTTTTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3261 CCTAGAGAAGG
FbaI 2030 TGATCA T^GATCA
3407 TGATCA
MfeI 2150 CAATTG C^AATTG
2302 CAATTG
MslI 1140 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2550 CATGGTTATG
MunI 2150 CAATTG C^AATTG
2302 CAATTG
PflMI 1263 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1275 CCATCCTCTGG
PvuII 1744 CAGCTG CAG^CTG
3517 CAGCTG
RcaI 2363 TCATGA T^CATGA
3249 TCATGA
SnaBI 2488 TACGTA TAC^GTA
2528 TACGTA
SspBI 1361 TGTACA T^GTACA
1465 TGTACA
StuI 708 AGGCCT AGG^CCT
1179 AGGCCT
Van91I 1263 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1275 CCATCCTCTGG
XbaI 3756 TCTAGA T^CTAGA
5172 TCTAGA
XcmI 3017 CCACGGCTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4792 CCAAATAAATACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AvaII BalI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglII BmyI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII