##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspI AviII BamHI BcgI
BfrI BglI BseAI BsiWI BsmBI
BspEI BspLU11I BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI FspI HpaI
KspI MluI MroI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PmeI PshAI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 4822 GTAGAC GT^[AC][GT]AC
AflIII 1322 ACACGT A^C[GA][CT]GT
AgeI 1639 ACCGGT A^CCGGT
AvrII 3222 CCTAGG C^CTAGG
BbvCI 3923 CCTCAGC CCTCAGC(-5/-2)
BlnI 3222 CCTAGG C^CTAGG
BlpI 164 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 164 GCTGAGC GC^T[ACGT]AGC
BsaBI 981 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 4809 GAGGAG GAGGAG(10/8)
BsiEI 202 CGGTCG CG[GA][CT]^CG
Bsp1407I 4274 TGTACA T^GTACA
BspDI 3591 ATCGAT AT^CGAT
BspMI 4964 ACCTGC ACCTGC(4/8)
BsrDI 5007 CATTGC GCAATG(2/0)
BsrFI 1639 ACCGGT [GA]^CCGG[CT]
BsrGI 4274 TGTACA T^GTACA
BstAPI 3264 GCACTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 164 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1639 ACCGGT [GA]^CCGG[CT]
ClaI 3591 ATCGAT AT^CGAT
EcoT22I 3449 ATGCAT ATGCA^T
MamI 981 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3197 CAATTG C^AATTG
MunI 3197 CAATTG C^AATTG
NsiI 3449 ATGCAT ATGCA^T
PinAI 1639 ACCGGT A^CCGGT
SacI 2478 GAGCTC GAGCT^C
ScaI 2014 AGTACT AGT^ACT
SmlI 1028 CTTGAG C^T[CT][GA]AG
SnaBI 4369 TACGTA TAC^GTA
SpeI 2781 ACTAGT A^CTAGT
SphI 1471 GCATGC GCATG^C
SspBI 4274 TGTACA T^GTACA
SspI 1548 AATATT AAT^ATT
XcmI 3766 CCAGAGCAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 583 GGTACC G^GTACC
1813 GGTACC
AccII 69 CGCG CG^CG
120 CGCG
AcyI 53 GGCGCC G[GA]^CG[CT]C
60 GGCGCC
AlwNI 425 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3663 CAGGTCCTG
Asp718I 583 GGTACC G^GTACC
1813 GGTACC
AspHI 1031 GAGCAC G[AT]GC[AT]^C
2478 GAGCTC
AvaI 2129 CTCGGG C^[CT]CG[GA]G
2933 CTCGGG
BalI 938 TGGCCA TGG^CCA
4028 TGGCCA
BbeI 53 GGCGCC GGCGC^C
60 GGCGCC
BbrPI 754 CACGTG CAC^GTG
1323 CACGTG
BciVI 2504 GGATAC GTATCC(6/5)
4693 GTATCC
BsaHI 53 GGCGCC G[GA]^CG[CT]C
60 GGCGCC
BsaWI 1639 ACCGGT [AT]^CCGG[AT]
1816 ACCGGA
BsiHKAI 1031 GAGCAC G[AT]GC[AT]^C
2478 GAGCTC
BsoBI 2129 CTCGGG C^[CT]CG[GA]G
2933 CTCGGG
BspHI 1420 TCATGA T^CATGA
3203 TCATGA
BsrBI 38 CCGCTC CCGCTC(-3/-3)
195 CCGCTC
BstUI 69 CGCG CG^CG
120 CGCG
DraI 2959 TTTAAA TTT^AAA
4120 TTTAAA
DraIII 2541 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3518 CACTGGGTG
EaeI 938 TGGCCA [CT]^GGCC[GA]
4028 TGGCCA
EcoNI 2318 CCTAGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3808 CCTCAGGCAGG
EcoRI 1687 GAATTC G^AATTC
3421 GAATTC
EcoRV 348 GATATC GAT^ATC
996 GATATC
HgaI 118 GACGC GACGC(5/10)
2749 GCGTC
Hin1I 53 GGCGCC G[GA]^CG[CT]C
60 GGCGCC
HindIII 766 AAGCTT A^AGCTT
1570 AAGCTT
KasI 53 GGCGCC G^GCGCC
60 GGCGCC
KpnI 583 GGTACC GGTAC^C
1813 GGTACC
MluNI 938 TGGCCA TGG^CCA
4028 TGGCCA
MscI 938 TGGCCA TGG^CCA
4028 TGGCCA
MslI 2682 CATAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3477 CATCTTTATG
MvnI 69 CGCG CG^CG
120 CGCG
NarI 53 GGCGCC GG^CGCC
60 GGCGCC
PmaCI 754 CACGTG CAC^GTG
1323 CACGTG
PmlI 754 CACGTG CAC^GTG
1323 CACGTG
PvuII 514 CAGCTG CAG^CTG
4941 CAGCTG
RcaI 1420 TCATGA T^CATGA
3203 TCATGA
SapI 2475 GAAGAGC GCTCTTC(1/4)
3023 GAAGAGC
SfoI 53 GGCGCC GGC^GCC
60 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AseI AsnI Asp700I
AvaII BanI BanII BbsI BbvI
BclI BcnI BfaI BglII BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII XmnI