##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AviII BbrPI BfrI BsaAI BsaBI
BsiWI BspDI BsrBI BsrFI BssSI
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
FseI FspI MamI MluI NaeI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RsrII SalI SapI SbfI
ScaI SexAI SfiI SgrAI SnaBI
Sse8387I SspI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 4539 GGTACC G^GTACC
AccI 1849 GTATAC GT^[AC][GT]AC
AccIII 18 TCCGGA T^CCGGA
ApaI 3511 GGGCCC GGGCC^C
Asp700I 2257 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4539 GGTACC G^GTACC
AspI 4330 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4263 CCTAGG C^CTAGG
BbeI 3494 GGCGCC GGCGC^C
BcgI 4245 GCATGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4151 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4263 CCTAGG C^CTAGG
BlpI 159 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 159 GCTCAGC GC^T[ACGT]AGC
BsaWI 18 TCCGGA [AT]^CCGG[AT]
BseAI 18 TCCGGA T^CCGGA
BsiEI 729 CGATCG CG[GA][CT]^CG
BspEI 18 TCCGGA T^CCGGA
BssHII 143 GCGCGC G^CGCGC
Bst1107I 1849 GTATAC GTA^TAC
BstEII 3723 GGTCACC G^GT[ACGT]ACC
BstPI 3723 GGTCACC G^GT[ACGT]ACC
BstZ17I 1849 GTATAC GTA^TAC
CelII 159 GCTCAGC GC^T[ACGT]AGC
DraI 4523 TTTAAA TTT^AAA
DrdI 93 GACATCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2160 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3723 GGTCACC G^GT[ACGT]ACC
EcoT22I 1411 ATGCAT ATGCA^T
HgaI 53 GCGTC GACGC(5/10)
KasI 3494 GGCGCC G^GCGCC
KpnI 4539 GGTACC GGTAC^C
KspI 26 CCGCGG CCGC^GG
MfeI 2535 CAATTG C^AATTG
MroI 18 TCCGGA T^CCGGA
MunI 2535 CAATTG C^AATTG
NarI 3494 GGCGCC GG^CGCC
NcoI 2409 CCATGG C^CATGG
NdeI 3822 CATATG CA^TATG
NheI 1264 GCTAGC G^CTAGC
NsiI 1411 ATGCAT ATGCA^T
PflFI 4330 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1001 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2375 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 729 CGATCG CGAT^CG
SacI 2776 GAGCTC GAGCT^C
SacII 26 CCGCGG CCGC^GG
SfoI 3494 GGCGCC GGC^GCC
SmaI 1877 CCCGGG CCC^GGG
SmlI 3460 CTCAAG C^T[CT][GA]AG
SphI 4208 GCATGC GCATG^C
StuI 1359 AGGCCT AGG^CCT
SwaI 4522 ATTTAAAT ATTT^AAAT
Tth111I 4330 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1877 CCCGGG C^CCGGG
XmaI 1877 CCCGGG C^CCGGG
XmnI 2257 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 52 GGCGTC G[GA]^CG[CT]C
3494 GGCGCC
AflIII 1613 ACATGT A^C[GA][CT]GT
1620 ACATGT
Alw44I 237 GTGCAC G^TGCAC
3412 GTGCAC
AlwNI 304 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
3028 CAGGCTCTG
AocI 1355 CCTCAGG CC^T[ACGT]AGG
3520 CCTGAGG
ApaLI 237 GTGCAC G^TGCAC
3412 GTGCAC
BamHI 185 GGATCC G^GATCC
2357 GGATCC
BbvCI 909 CCTCAGC CCTCAGC(-5/-2)
4239 GCTGAGG
BciVI 2529 GGATAC GTATCC(6/5)
2554 GTATCC
BsaHI 52 GGCGTC G[GA]^CG[CT]C
3494 GGCGCC
BsgI 1951 CTGCAC GTGCAG(16/14)
2510 CTGCAC
BsmBI 315 CGTCTC CGTCTC(1/5)
4169 CGTCTC
Bsp1407I 1255 TGTACA T^GTACA
1295 TGTACA
BspLU11I 1613 ACATGT A^CATGT
1620 ACATGT
BsrDI 380 CATTGC GCAATG(2/0)
2580 CATTGC
BsrGI 1255 TGTACA T^GTACA
1295 TGTACA
BstBI 1799 TTCGAA TT^CGAA
2923 TTCGAA
Bsu36I 1355 CCTCAGG CC^T[ACGT]AGG
3520 CCTGAGG
DraIII 1954 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3674 CACTCAGTG
EarI 3886 CTCTTC CTCTTC(1/4)
3931 CTCTTC
Eco81I 1355 CCTCAGG CC^T[ACGT]AGG
3520 CCTGAGG
Hin1I 52 GGCGTC G[GA]^CG[CT]C
3494 GGCGCC
HindIII 358 AAGCTT A^AGCTT
3121 AAGCTT
HpaI 120 GTTAAC GTT^AAC
507 GTTAAC
Ksp632I 3886 CTCTTC CTCTTC(1/4)
3931 CTCTTC
NspV 1799 TTCGAA TT^CGAA
2923 TTCGAA
PflMI 2178 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3673 CCACTCAGTGG
SfuI 1799 TTCGAA TT^CGAA
2923 TTCGAA
SpeI 193 ACTAGT A^CTAGT
1974 ACTAGT
SspBI 1255 TGTACA T^GTACA
1295 TGTACA
Van91I 2178 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3673 CCACTCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BclI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII