##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
Acc65I AclI AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AviII BcgI BglII BsaI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI Eam1105I Eco47III FspI
HpaI KpnI NdeI NotI NruI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SapI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3397 GACGTC GACGT^C
AflII 3691 CTTAAG C^TTAAG
Alw44I 3996 GTGCAC G^TGCAC
AocI 1281 CCTGAGG CC^T[ACGT]AGG
ApaLI 3996 GTGCAC G^TGCAC
Asp700I 2384 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3712 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2665 CCTAGG C^CTAGG
BbrPI 2701 CACGTG CAC^GTG
BclI 2760 TGATCA T^GATCA
BfrI 3691 CTTAAG C^TTAAG
BlnI 2665 CCTAGG C^CTAGG
BsaAI 2701 CACGTG [CT]AC^GT[GA]
BssSI 1550 CACGAG CACGAG(-5/-1)
Bsu36I 1281 CCTGAGG CC^T[ACGT]AGG
DraIII 3122 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3397 GACGTCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1281 CCTGAGG CC^T[ACGT]AGG
EcoRI 2306 GAATTC G^AATTC
EcoRV 3016 GATATC GAT^ATC
FbaI 2760 TGATCA T^GATCA
FseI 77 GGCCGGCC GGCCGG^CC
HincII 858 GTCGAC GT[CT]^[GA]AC
HindII 858 GTCGAC GT[CT]^[GA]AC
HindIII 1082 AAGCTT A^AGCTT
KspI 547 CCGCGG CCGC^GG
MfeI 2420 CAATTG C^AATTG
MluI 350 ACGCGT A^CGCGT
MunI 2420 CAATTG C^AATTG
NheI 2500 GCTAGC G^CTAGC
PflFI 3712 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4051 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2701 CACGTG CAC^GTG
PmlI 2701 CACGTG CAC^GTG
PpuMI 2266 GGGACCC [GA]G^G[AT]CC[CT]
SacI 1427 GAGCTC GAGCT^C
SacII 547 CCGCGG CCGC^GG
SalI 858 GTCGAC G^TCGAC
ScaI 1645 AGTACT AGT^ACT
SexAI 3123 ACCTGGT A^CC[AT]GGT
SspI 1994 AATATT AAT^ATT
StuI 2668 AGGCCT AGG^CCT
Tth111I 3712 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4051 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2384 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 858 GTCGAC GT^[AC][GT]AC
2148 GTCTAC
AcsI 2306 GAATTC [GA]^AATT[CT]
3817 AAATTT
AflIII 350 ACGCGT A^C[GA][CT]GT
2702 ACGTGT
ApoI 2306 GAATTC [GA]^AATT[CT]
3817 AAATTT
BamHI 1868 GGATCC G^GATCC
2216 GGATCC
BbeI 202 GGCGCC GGCGC^C
483 GGCGCC
BbsI 209 GTCTTC GAAGAC(2/6)
3236 GTCTTC
BglI 267 GCCTGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
453 GCCAGCGGGGC
BpuAI 209 GTCTTC GAAGAC(2/6)
3236 GTCTTC
BsaBI 1905 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3595 GATGATGATC
BspHI 1148 TCATGA T^CATGA
3020 TCATGA
BstAPI 1237 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2191 GCATCGCATGC
BstEII 1050 GGTTACC G^GT[ACGT]ACC
2660 GGTCACC
BstPI 1050 GGTTACC G^GT[ACGT]ACC
2660 GGTCACC
DraI 109 TTTAAA TTT^AAA
4349 TTTAAA
EcoO65I 1050 GGTTACC G^GT[ACGT]ACC
2660 GGTCACC
EcoT22I 2189 ATGCAT ATGCA^T
4039 ATGCAT
HaeII 202 GGCGCC [GA]GCGC^[CT]
483 GGCGCC
KasI 202 GGCGCC G^GCGCC
483 GGCGCC
MamI 1905 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3595 GATGATGATC
NarI 202 GGCGCC GG^CGCC
483 GGCGCC
NsiI 2189 ATGCAT ATGCA^T
4039 ATGCAT
RcaI 1148 TCATGA T^CATGA
3020 TCATGA
SfoI 202 GGCGCC GGC^GCC
483 GGCGCC
SmaI 479 CCCGGG CCC^GGG
800 CCCGGG
SphI 1461 GCATGC GCATG^C
2196 GCATGC
XbaI 3522 TCTAGA T^CTAGA
3785 TCTAGA
XmaCI 479 CCCGGG C^CCGGG
800 CCCGGG
XmaI 479 CCCGGG C^CCGGG
800 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BciVI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PaeR7I PleI PstI
PvuII RsaI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmlI Sse8387I
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoI XhoII