##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AclI AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I AspEI
AviII BbeI BbrPI BsaBI BsiEI
BsiWI BspDI BspLU11I BstBI BstEII
BstPI ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI KasI
KspI MamI MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SfoI SfuI SmaI
SpeI SwaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 946 GGTACC G^GTACC
AccIII 3212 TCCGGA T^CCGGA
AflII 2109 CTTAAG C^TTAAG
ApaI 754 GGGCCC GGGCC^C
Asp718I 946 GGTACC G^GTACC
AvrII 4576 CCTAGG C^CTAGG
BamHI 421 GGATCC G^GATCC
BfrI 2109 CTTAAG C^TTAAG
BlnI 4576 CCTAGG C^CTAGG
BsaWI 3212 TCCGGA [AT]^CCGG[AT]
BseAI 3212 TCCGGA T^CCGGA
BsmI 598 GAATGC GAATGC(1/-1)
Bsp1407I 4670 TGTACA T^GTACA
BspEI 3212 TCCGGA T^CCGGA
BspHI 4427 TCATGA T^CATGA
BsrGI 4670 TGTACA T^GTACA
BssHII 2415 GCGCGC G^CGCGC
BssSI 2020 CTCGTG CACGAG(-5/-1)
Bst1107I 2154 GTATAC GTA^TAC
BstZ17I 2154 GTATAC GTA^TAC
DrdI 3109 GACCACGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4594 GAATTC G^AATTC
EcoT22I 3765 ATGCAT ATGCA^T
HaeII 181 AGCGCC [GA]GCGC^[CT]
HgaI 4116 GCGTC GACGC(5/10)
HpaI 4525 GTTAAC GTT^AAC
KpnI 946 GGTACC GGTAC^C
MroI 3212 TCCGGA T^CCGGA
NdeI 1047 CATATG CA^TATG
NruI 2487 TCGCGA TCG^CGA
NsiI 3765 ATGCAT ATGCA^T
PshAI 4628 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4427 TCATGA T^CATGA
SalI 239 GTCGAC G^TCGAC
SapI 4293 GCTCTTC GCTCTTC(1/4)
SbfI 1370 CCTGCAGG CCTGCA^GG
ScaI 4639 AGTACT AGT^ACT
SexAI 3977 ACCTGGT A^CC[AT]GGT
SfiI 729 GGCCCCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 2147 TACGTA TAC^GTA
Sse8387I 1370 CCTGCAGG CCTGCA^GG
SspBI 4670 TGTACA T^GTACA
XbaI 3830 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 4044 GACGTC GACGT^C
4369 GACGTC
AccI 239 GTCGAC GT^[AC][GT]AC
2154 GTATAC
Alw44I 2996 GTGCAC G^TGCAC
3758 GTGCAC
ApaLI 2996 GTGCAC G^TGCAC
3758 GTGCAC
AvaI 65 CCCGAG C^[CT]CG[GA]G
362 CCCGAG
BsaAI 2147 TACGTA [CT]AC^GT[GA]
3112 CACGTA
BsaI 3317 GAGACC GGTCTC(1/5)
3 GGTCTC
BsmBI 2895 CGTCTC CGTCTC(1/5)
1973 GAGACG
BsoBI 65 CCCGAG C^[CT]CG[GA]G
362 CCCGAG
BsrBI 1237 CCGCTC CCGCTC(-3/-3)
1288 CCGCTC
BsrDI 1418 GCAATG GCAATG(2/0)
1486 GCAATG
EcoNI 1604 CCTTAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4021 CCTGGAGCAGG
HindIII 146 AAGCTT A^AGCTT
3660 AAGCTT
SacI 2499 GAGCTC GAGCT^C
4291 GAGCTC
SgrAI 302 CACCGGCG C[GA]^CCGG[CT]G
4472 CACCGGCG
SmlI 1282 CTTGAG C^T[CT][GA]AG
2109 CTTAAG
SphI 1037 GCATGC GCATG^C
1730 GCATGC
SspI 71 AATATT AAT^ATT
4879 AATATT
StuI 2852 AGGCCT AGG^CCT
4804 AGGCCT
TfiI 836 GATTC G^A[AT]TC
4587 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AspI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII