################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AclI AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AviII BbeI BbrPI BsaBI BsiEI BsiWI BspDI BspLU11I BstBI BstEII BstPI ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI KasI KspI MamI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SfoI SfuI SmaI SpeI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 946 GGTACC G^GTACC AccIII 3212 TCCGGA T^CCGGA AflII 2109 CTTAAG C^TTAAG ApaI 754 GGGCCC GGGCC^C Asp718I 946 GGTACC G^GTACC AvrII 4576 CCTAGG C^CTAGG BamHI 421 GGATCC G^GATCC BfrI 2109 CTTAAG C^TTAAG BlnI 4576 CCTAGG C^CTAGG BsaWI 3212 TCCGGA [AT]^CCGG[AT] BseAI 3212 TCCGGA T^CCGGA BsmI 598 GAATGC GAATGC(1/-1) Bsp1407I 4670 TGTACA T^GTACA BspEI 3212 TCCGGA T^CCGGA BspHI 4427 TCATGA T^CATGA BsrGI 4670 TGTACA T^GTACA BssHII 2415 GCGCGC G^CGCGC BssSI 2020 CTCGTG CACGAG(-5/-1) Bst1107I 2154 GTATAC GTA^TAC BstZ17I 2154 GTATAC GTA^TAC DrdI 3109 GACCACGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 4594 GAATTC G^AATTC EcoT22I 3765 ATGCAT ATGCA^T HaeII 181 AGCGCC [GA]GCGC^[CT] HgaI 4116 GCGTC GACGC(5/10) HpaI 4525 GTTAAC GTT^AAC KpnI 946 GGTACC GGTAC^C MroI 3212 TCCGGA T^CCGGA NdeI 1047 CATATG CA^TATG NruI 2487 TCGCGA TCG^CGA NsiI 3765 ATGCAT ATGCA^T PshAI 4628 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 4427 TCATGA T^CATGA SalI 239 GTCGAC G^TCGAC SapI 4293 GCTCTTC GCTCTTC(1/4) SbfI 1370 CCTGCAGG CCTGCA^GG ScaI 4639 AGTACT AGT^ACT SexAI 3977 ACCTGGT A^CC[AT]GGT SfiI 729 GGCCCCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SnaBI 2147 TACGTA TAC^GTA Sse8387I 1370 CCTGCAGG CCTGCA^GG SspBI 4670 TGTACA T^GTACA XbaI 3830 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AatII 4044 GACGTC GACGT^C 4369 GACGTC AccI 239 GTCGAC GT^[AC][GT]AC 2154 GTATAC Alw44I 2996 GTGCAC G^TGCAC 3758 GTGCAC ApaLI 2996 GTGCAC G^TGCAC 3758 GTGCAC AvaI 65 CCCGAG C^[CT]CG[GA]G 362 CCCGAG BsaAI 2147 TACGTA [CT]AC^GT[GA] 3112 CACGTA BsaI 3317 GAGACC GGTCTC(1/5) 3 GGTCTC BsmBI 2895 CGTCTC CGTCTC(1/5) 1973 GAGACG BsoBI 65 CCCGAG C^[CT]CG[GA]G 362 CCCGAG BsrBI 1237 CCGCTC CCGCTC(-3/-3) 1288 CCGCTC BsrDI 1418 GCAATG GCAATG(2/0) 1486 GCAATG EcoNI 1604 CCTTAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4021 CCTGGAGCAGG HindIII 146 AAGCTT A^AGCTT 3660 AAGCTT SacI 2499 GAGCTC GAGCT^C 4291 GAGCTC SgrAI 302 CACCGGCG C[GA]^CCGG[CT]G 4472 CACCGGCG SmlI 1282 CTTGAG C^T[CT][GA]AG 2109 CTTAAG SphI 1037 GCATGC GCATG^C 1730 GCATGC SspI 71 AATATT AAT^ATT 4879 AATATT StuI 2852 AGGCCT AGG^CCT 4804 AGGCCT TfiI 836 GATTC G^A[AT]TC 4587 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AspHI AspI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII