##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
Aor51HI AscI Asp700I Asp718I AspI
AviII AvrII BbeI BclI BlnI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrFI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoNI
FbaI FseI FspI HgaI HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
StuI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 920 GACGTC GACGT^C
AccII 135 CGCG CG^CG
AcyI 920 GACGTC G[GA]^CG[CT]C
AflII 1645 CTTAAG C^TTAAG
Alw44I 2650 GTGCAC G^TGCAC
ApaLI 2650 GTGCAC G^TGCAC
BamHI 1712 GGATCC G^GATCC
BbrPI 2647 CACGTG CAC^GTG
BcgI 1890 CGATGGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1645 CTTAAG C^TTAAG
BsaAI 2647 CACGTG [CT]AC^GT[GA]
BsaBI 3584 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 920 GACGTC G[GA]^CG[CT]C
BsmI 2408 GCATTC GAATGC(1/-1)
BspHI 2704 TCATGA T^CATGA
BsrBI 962 CCGCTC CCGCTC(-3/-3)
BstAPI 2874 GCAAGCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 709 GGTGACC G^GT[ACGT]ACC
BstPI 709 GGTGACC G^GT[ACGT]ACC
BstUI 135 CGCG CG^CG
DsaI 480 CCGTGG C^C[GA][CT]GG
EcoO65I 709 GGTGACC G^GT[ACGT]ACC
HaeII 1520 GGCGCT [GA]GCGC^[CT]
HapII 1328 CCGG C^CGG
Hin1I 920 GACGTC G[GA]^CG[CT]C
HpaII 1328 CCGG C^CGG
MamI 3584 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1328 CCGG C^CGG
MvnI 135 CGCG CG^CG
NdeI 2905 CATATG CA^TATG
PacI 2297 TTAATTAA TTAAT^TAA
PflMI 633 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2647 CACGTG CAC^GTG
PmlI 2647 CACGTG CAC^GTG
RcaI 2704 TCATGA T^CATGA
SmaI 1327 CCCGGG CCC^GGG
SpeI 472 ACTAGT A^CTAGT
SspI 3422 AATATT AAT^ATT
Van91I 633 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1327 CCCGGG C^CCGGG
XmaI 1327 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 2287 ACATGT A^C[GA][CT]GT
3542 ACATGT
AhdI 115 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1031 GACCTGGTGTC
AocI 704 CCTGAGG CC^T[ACGT]AGG
2068 CCTCAGG
ApaI 1330 GGGCCC GGGCC^C
1338 GGGCCC
AspEI 115 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1031 GACCTGGTGTC
AvaI 1327 CCCGGG C^[CT]CG[GA]G
4580 CTCGGG
BalI 1063 TGGCCA TGG^CCA
1316 TGGCCA
BbsI 22 GAAGAC GAAGAC(2/6)
4935 GTCTTC
BbvCI 909 CCTCAGC CCTCAGC(-5/-2)
609 GCTGAGG
BcnI 1327 CCCGG CC^[GC]GG
1328 CCGGG
BglI 103 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1264 GCCTAGTGGGC
BpuAI 22 GAAGAC GAAGAC(2/6)
4935 GTCTTC
BsaI 223 GAGACC GGTCTC(1/5)
1069 GGTCTC
BsgI 1044 CTGCAC GTGCAG(16/14)
4525 GTGCAG
BsoBI 1327 CCCGGG C^[CT]CG[GA]G
4580 CTCGGG
Bsp1407I 141 TGTACA T^GTACA
2284 TGTACA
BspLU11I 2287 ACATGT A^CATGT
3542 ACATGT
BsrGI 141 TGTACA T^GTACA
2284 TGTACA
BstXI 1026 CCAAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1097 CCAATTAACTGG
Bsu36I 704 CCTGAGG CC^T[ACGT]AGG
2068 CCTCAGG
CfoI 15 GCGC GCG^C
1521 GCGC
DraIII 1839 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4272 CACAATGTG
Eam1105I 115 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1031 GACCTGGTGTC
Eco81I 704 CCTGAGG CC^T[ACGT]AGG
2068 CCTCAGG
EcoRI 1987 GAATTC G^AATTC
4175 GAATTC
EcoRV 3497 GATATC GAT^ATC
3867 GATATC
FauI 278 GCGGG CCCGC(4/6)
2688 GCGGG
HhaI 15 GCGC GCG^C
1521 GCGC
HinP1I 15 GCGC G^CGC
1521 GCGC
HincII 123 GTCAAC GT[CT]^[GA]AC
4875 GTTGAC
HindII 123 GTCAAC GT[CT]^[GA]AC
4875 GTTGAC
HindIII 2541 AAGCTT A^AGCTT
4356 AAGCTT
MluNI 1063 TGGCCA TGG^CCA
1316 TGGCCA
MscI 1063 TGGCCA TGG^CCA
1316 TGGCCA
NciI 1327 CCCGG CC^[GC]GG
1328 CCGGG
PpuMI 669 GGGACCT [GA]G^G[AT]CC[CT]
1380 AGGTCCT
ScaI 4193 AGTACT AGT^ACT
4621 AGTACT
SexAI 677 ACCTGGT A^CC[AT]GGT
1032 ACCTGGT
SspBI 141 TGTACA T^GTACA
2284 TGTACA
TaqI 1889 TCGA T^CGA
2144 TCGA
TthHB8I 1889 TCGA T^CGA
2144 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BanI BanII BbvI BciVI
BfaI BglII BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII