##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AcyI AgeI AscI
Asp718I AviII AvrII BamHI BbeI
BbrPI BbvCI BlnI BlpI Bpu1102I
BsaAI BsaHI BsiWI BspDI BspMI
BssHII BstBI BstEII BstPI CelII
ClaI DrdI EagI EclXI Eco52I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HgaI Hin1I HindIII KasI
KpnI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PvuI
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 283 TCCGGA T^CCGGA
AclI 4581 AACGTT AA^CGTT
Alw44I 3584 GTGCAC G^TGCAC
AlwNI 1245 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 915 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2379 AGCGCT AGC^GCT
ApaI 127 GGGCCC GGGCC^C
ApaLI 3584 GTGCAC G^TGCAC
AvaI 130 CCCGGG C^[CT]CG[GA]G
BcgI 1154 CGAAGCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 892 GGATAC GTATCC(6/5)
BglI 2518 GCCGGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 214 AGATCT A^GATCT
BsaBI 1013 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 283 TCCGGA T^CCGGA
BsiEI 105 CGGTCG CG[GA][CT]^CG
BsmBI 1223 GAGACG CGTCTC(1/5)
BsoBI 130 CCCGGG C^[CT]CG[GA]G
BspEI 283 TCCGGA T^CCGGA
BsrDI 3301 CATTGC GCAATG(2/0)
BsrFI 4347 GCCGGT [GA]^CCGG[CT]
BssSI 36 CTCGTG CACGAG(-5/-1)
Bst1107I 4212 GTATAC GTA^TAC
BstAPI 3253 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4212 GTATAC GTA^TAC
Bsu36I 915 CCTGAGG CC^T[ACGT]AGG
Cfr10I 4347 GCCGGT [GA]^CCGG[CT]
CpoI 171 CGGACCG CG^G[AT]CCG
DraII 4468 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 2589 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2379 AGCGCT AGC^GCT
Eco81I 915 CCTGAGG CC^T[ACGT]AGG
EcoO109I 4468 AGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 2379 AGCGCT [GA]GCGC^[CT]
HpaI 3079 GTTAAC GTT^AAC
MamI 1013 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 283 TCCGGA T^CCGGA
NcoI 530 CCATGG C^CATGG
NdeI 3915 CATATG CA^TATG
PacI 3758 TTAATTAA TTAAT^TAA
Psp1406I 4581 AACGTT AA^CGTT
RsrII 171 CGGACCG CG^G[AT]CCG
SapI 564 GAAGAGC GCTCTTC(1/4)
ScaI 2259 AGTACT AGT^ACT
SmaI 130 CCCGGG CCC^GGG
SphI 3271 GCATGC GCATG^C
StuI 912 AGGCCT AGG^CCT
XcmI 1550 CCAGGAGAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 130 CCCGGG C^CCGGG
XmaI 130 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflII 3822 CTTAAG C^TTAAG
4133 CTTAAG
AflIII 404 ACATGT A^C[GA][CT]GT
2933 ACATGT
AhdI 1429 GACTCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3679 GACACATAGTC
AseI 2773 ATTAAT AT^TAAT
3757 ATTAAT
AsnI 2773 ATTAAT AT^TAAT
3757 ATTAAT
AspEI 1429 GACTCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3679 GACACATAGTC
AspI 4236 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4354 GACGTGGTC
BalI 814 TGGCCA TGG^CCA
4381 TGGCCA
BclI 1352 TGATCA T^GATCA
1489 TGATCA
BfrI 3822 CTTAAG C^TTAAG
4133 CTTAAG
BsaI 2016 GAGACC GGTCTC(1/5)
2391 GGTCTC
BsaWI 283 TCCGGA [AT]^CCGG[AT]
515 TCCGGT
BsgI 1903 CTGCAC GTGCAG(16/14)
3589 CTGCAC
BspHI 482 TCATGA T^CATGA
823 TCATGA
BspLU11I 404 ACATGT A^CATGT
2933 ACATGT
BsrBI 2307 CCGCTC CCGCTC(-3/-3)
289 GAGCGG
Eam1105I 1429 GACTCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3679 GACACATAGTC
EcoRI 253 GAATTC G^AATTC
4475 GAATTC
FbaI 1352 TGATCA T^GATCA
1489 TGATCA
KspI 55 CCGCGG CCGC^GG
168 CCGCGG
MluNI 814 TGGCCA TGG^CCA
4381 TGGCCA
MscI 814 TGGCCA TGG^CCA
4381 TGGCCA
PflFI 4236 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4354 GACGTGGTC
PshAI 354 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4651 GACTGTAGTC
PshBI 2773 ATTAAT AT^TAAT
3757 ATTAAT
PvuII 393 CAGCTG CAG^CTG
1366 CAGCTG
RcaI 482 TCATGA T^CATGA
823 TCATGA
SacI 1983 GAGCTC GAGCT^C
2481 GAGCTC
SacII 55 CCGCGG CCGC^GG
168 CCGCGG
TaqI 2414 TCGA T^CGA
3293 TCGA
TfiI 479 GATTC G^A[AT]TC
820 GAATC
Tth111I 4236 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4354 GACGTGGTC
TthHB8I 2414 TCGA T^CGA
3293 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI