##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AclI        AgeI        AocI        AscI        AviII       
AvrII       BamHI       BglII       BlnI        BsaBI       
BsiEI       BsiWI       BsmI        BspDI       BspLU11I    
BsrDI       BssHII      Bst1107I    BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        DrdI        EagI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FseI        FspI        HindIII     
HpaI        MamI        MfeI        MluI        MunI        
NdeI        NheI        NotI        NsiI        PacI        
PinAI       PmeI        PshAI       Psp1406I    PvuI        
PvuII       SalI        SbfI        ScaI        SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        2432  GACGTC               GACGT^C
Acc65I        480  GGTACC               G^GTACC
AccIII       1194  TCCGGA               T^CCGGA
AflII        3673  CTTAAG               C^TTAAG
Alw44I       2816  GTGCAC               G^TGCAC
ApaI          126  GGGCCC               GGGCC^C
ApaLI        2816  GTGCAC               G^TGCAC
Asp700I      1681  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       480  GGTACC               G^GTACC
BbeI         1417  GGCGCC               GGCGC^C
BcgI         4332  CGATCCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         949  GGATAC               GTATCC(6/5)
BfrI         3673  CTTAAG               C^TTAAG
BlpI          886  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      886  GCTGAGC              GC^T[ACGT]AGC
BsaI         4309  GGTCTC               GGTCTC(1/5)
BseAI        1194  TCCGGA               T^CCGGA
Bsp1407I     3967  TGTACA               T^GTACA
BspEI        1194  TCCGGA               T^CCGGA
BspHI        4116  TCATGA               T^CATGA
BsrGI        3967  TGTACA               T^GTACA
BstAPI       3242  GCAGTGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        3131  TTCGAA               TT^CGAA
CelII         886  GCTGAGC              GC^T[ACGT]AGC
CpoI         2603  CGGTCCG              CG^G[AT]CCG
KasI         1417  GGCGCC               G^GCGCC
KpnI          480  GGTACC               GGTAC^C
MroI         1194  TCCGGA               T^CCGGA
NarI         1417  GGCGCC               GG^CGCC
NcoI         2850  CCATGG               C^CATGG
NruI         3176  TCGCGA               TCG^CGA
NspI         2412  GCATGT               [GA]CATG^[CT]
NspV         3131  TTCGAA               TT^CGAA
PaeR7I        329  CTCGAG               C^TCGAG
RcaI         4116  TCATGA               T^CATGA
RsrII        2603  CGGTCCG              CG^G[AT]CCG
SexAI        2160  ACCAGGT              A^CC[AT]GGT
SfiI         2020  GGCCTTGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1417  GGCGCC               GGC^GCC
SfuI         3131  TTCGAA               TT^CGAA
SgrAI          44  CGCCGGCG             C[GA]^CCGG[CT]G
SspBI        3967  TGTACA               T^GTACA
XbaI         4772  TCTAGA               T^CTAGA
XhoI          329  CTCGAG               C^TCGAG
XmnI         1681  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         1726  GTCTAC               GT^[AC][GT]AC
             3098  GTCTAC              
Aor51HI       903  AGCGCT               AGC^GCT
             3400  AGCGCT              
AseI         3282  ATTAAT               AT^TAAT
             3765  ATTAAT              
AsnI         3282  ATTAAT               AT^TAAT
             3765  ATTAAT              
AspI         1570  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4281  GACAGGGTC           
BbrPI        2819  CACGTG               CAC^GTG
             3185  CACGTG              
BbsI         2528  GAAGAC               GAAGAC(2/6)
             3260  GAAGAC              
BpuAI        2528  GAAGAC               GAAGAC(2/6)
             3260  GAAGAC              
BsaWI         762  TCCGGT               [AT]^CCGG[AT]
             1194  TCCGGA              
BsmBI        2845  CGTCTC               CGTCTC(1/5)
             4120  GAGACG              
DraI         2477  TTTAAA               TTT^AAA
             4649  TTTAAA              
Eco47III      903  AGCGCT               AGC^GCT
             3400  AGCGCT              
HincII        526  GTTGAC               GT[CT]^[GA]AC
             3855  GTTGAC              
HindII        526  GTTGAC               GT[CT]^[GA]AC
             3855  GTTGAC              
NaeI           45  GCCGGC               GCC^GGC
             1308  GCCGGC              
NgoMIV         45  GCCGGC               G^CCGGC
             1308  GCCGGC              
PflFI        1570  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4281  GACAGGGTC           
PflMI         214  CCAGATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2161  CCAGGTCCTGG         
PmaCI        2819  CACGTG               CAC^GTG
             3185  CACGTG              
PmlI         2819  CACGTG               CAC^GTG
             3185  CACGTG              
PpuMI        1454  AGGACCC              [GA]G^G[AT]CC[CT]
             2163  AGGTCCT             
PshBI        3282  ATTAAT               AT^TAAT
             3765  ATTAAT              
SacI          258  GAGCTC               GAGCT^C
             2050  GAGCTC              
SapI         1040  GAAGAGC              GCTCTTC(1/4)
             2659  GAAGAGC             
Tth111I      1570  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4281  GACAGGGTC           
Van91I        214  CCAGATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2161  CCAGGTCCTGG         
XcmI          682  CCATAACCACATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2841  CCAGCGTCTCCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AhdI        AluI        AlwI        AlwNI       
ApoI        AspEI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvCI       BbvI        
BclI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaAI       BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BssSI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        Eam1105I    
EarI        EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        RsaI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII