##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AgeI AocI AscI AviII
AvrII BamHI BglII BlnI BsaBI
BsiEI BsiWI BsmI BspDI BspLU11I
BsrDI BssHII Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI DrdI EagI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HindIII
HpaI MamI MfeI MluI MunI
NdeI NheI NotI NsiI PacI
PinAI PmeI PshAI Psp1406I PvuI
PvuII SalI SbfI ScaI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2432 GACGTC GACGT^C
Acc65I 480 GGTACC G^GTACC
AccIII 1194 TCCGGA T^CCGGA
AflII 3673 CTTAAG C^TTAAG
Alw44I 2816 GTGCAC G^TGCAC
ApaI 126 GGGCCC GGGCC^C
ApaLI 2816 GTGCAC G^TGCAC
Asp700I 1681 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 480 GGTACC G^GTACC
BbeI 1417 GGCGCC GGCGC^C
BcgI 4332 CGATCCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 949 GGATAC GTATCC(6/5)
BfrI 3673 CTTAAG C^TTAAG
BlpI 886 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 886 GCTGAGC GC^T[ACGT]AGC
BsaI 4309 GGTCTC GGTCTC(1/5)
BseAI 1194 TCCGGA T^CCGGA
Bsp1407I 3967 TGTACA T^GTACA
BspEI 1194 TCCGGA T^CCGGA
BspHI 4116 TCATGA T^CATGA
BsrGI 3967 TGTACA T^GTACA
BstAPI 3242 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3131 TTCGAA TT^CGAA
CelII 886 GCTGAGC GC^T[ACGT]AGC
CpoI 2603 CGGTCCG CG^G[AT]CCG
KasI 1417 GGCGCC G^GCGCC
KpnI 480 GGTACC GGTAC^C
MroI 1194 TCCGGA T^CCGGA
NarI 1417 GGCGCC GG^CGCC
NcoI 2850 CCATGG C^CATGG
NruI 3176 TCGCGA TCG^CGA
NspI 2412 GCATGT [GA]CATG^[CT]
NspV 3131 TTCGAA TT^CGAA
PaeR7I 329 CTCGAG C^TCGAG
RcaI 4116 TCATGA T^CATGA
RsrII 2603 CGGTCCG CG^G[AT]CCG
SexAI 2160 ACCAGGT A^CC[AT]GGT
SfiI 2020 GGCCTTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1417 GGCGCC GGC^GCC
SfuI 3131 TTCGAA TT^CGAA
SgrAI 44 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 3967 TGTACA T^GTACA
XbaI 4772 TCTAGA T^CTAGA
XhoI 329 CTCGAG C^TCGAG
XmnI 1681 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1726 GTCTAC GT^[AC][GT]AC
3098 GTCTAC
Aor51HI 903 AGCGCT AGC^GCT
3400 AGCGCT
AseI 3282 ATTAAT AT^TAAT
3765 ATTAAT
AsnI 3282 ATTAAT AT^TAAT
3765 ATTAAT
AspI 1570 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4281 GACAGGGTC
BbrPI 2819 CACGTG CAC^GTG
3185 CACGTG
BbsI 2528 GAAGAC GAAGAC(2/6)
3260 GAAGAC
BpuAI 2528 GAAGAC GAAGAC(2/6)
3260 GAAGAC
BsaWI 762 TCCGGT [AT]^CCGG[AT]
1194 TCCGGA
BsmBI 2845 CGTCTC CGTCTC(1/5)
4120 GAGACG
DraI 2477 TTTAAA TTT^AAA
4649 TTTAAA
Eco47III 903 AGCGCT AGC^GCT
3400 AGCGCT
HincII 526 GTTGAC GT[CT]^[GA]AC
3855 GTTGAC
HindII 526 GTTGAC GT[CT]^[GA]AC
3855 GTTGAC
NaeI 45 GCCGGC GCC^GGC
1308 GCCGGC
NgoMIV 45 GCCGGC G^CCGGC
1308 GCCGGC
PflFI 1570 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4281 GACAGGGTC
PflMI 214 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2161 CCAGGTCCTGG
PmaCI 2819 CACGTG CAC^GTG
3185 CACGTG
PmlI 2819 CACGTG CAC^GTG
3185 CACGTG
PpuMI 1454 AGGACCC [GA]G^G[AT]CC[CT]
2163 AGGTCCT
PshBI 3282 ATTAAT AT^TAAT
3765 ATTAAT
SacI 258 GAGCTC GAGCT^C
2050 GAGCTC
SapI 1040 GAAGAGC GCTCTTC(1/4)
2659 GAAGAGC
Tth111I 1570 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4281 GACAGGGTC
Van91I 214 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2161 CCAGGTCCTGG
XcmI 682 CCATAACCACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2841 CCAGCGTCTCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApoI AspEI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI Eam1105I
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII