##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AhdI AscI Asp700I AspEI AspI
AvrII BamHI BbrPI BciVI BfrI
BglII BlnI BsaBI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI DraI Eam1105I EcoO65I
EcoRV HpaI MamI MfeI MluI
MroI MunI NheI NotI NruI
NspV PacI PflFI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
SalI SfiI SfuI SgrAI SnaBI
SpeI SspI SwaI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AgeI 3893 ACCGGT A^CCGGT
Alw44I 1411 GTGCAC G^TGCAC
ApaLI 1411 GTGCAC G^TGCAC
AseI 4426 ATTAAT AT^TAAT
AsnI 4426 ATTAAT AT^TAAT
AviII 3410 TGCGCA TGC^GCA
BbeI 367 GGCGCC GGCGC^C
BcgI 575 CGAGAGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2810 TGATCA T^GATCA
BsaAI 3545 CACGTA [CT]AC^GT[GA]
BsaI 411 GGTCTC GGTCTC(1/5)
BsmBI 3044 GAGACG CGTCTC(1/5)
Bsp1407I 2300 TGTACA T^GTACA
BsrGI 2300 TGTACA T^GTACA
BssSI 586 CACGAG CACGAG(-5/-1)
CpoI 68 CGGACCG CG^G[AT]CCG
DraIII 1594 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 928 GACGTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4224 GAATTC G^AATTC
EcoT22I 3064 ATGCAT ATGCA^T
FbaI 2810 TGATCA T^GATCA
FseI 2352 GGCCGGCC GGCCGG^CC
FspI 3410 TGCGCA TGC^GCA
HindIII 3514 AAGCTT A^AGCTT
KasI 367 GGCGCC G^GCGCC
NarI 367 GGCGCC GG^CGCC
NdeI 2394 CATATG CA^TATG
NsiI 3064 ATGCAT ATGCA^T
PinAI 3893 ACCGGT A^CCGGT
PshBI 4426 ATTAAT AT^TAAT
RsrII 68 CGGACCG CG^G[AT]CCG
ScaI 1517 AGTACT AGT^ACT
SfoI 367 GGCGCC GGC^GCC
SmaI 2094 CCCGGG CCC^GGG
SspBI 2300 TGTACA T^GTACA
StuI 2838 AGGCCT AGG^CCT
XmaCI 2094 CCCGGG C^CCGGG
XmaI 2094 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2051 GGTACC G^GTACC
2245 GGTACC
AcsI 1816 GAATTT [GA]^AATT[CT]
4224 GAATTC
AflIII 239 ACGTGT A^C[GA][CT]GT
1532 ACGTGT
AocI 3900 CCTCAGG CC^T[ACGT]AGG
4157 CCTAAGG
Aor51HI 3224 AGCGCT AGC^GCT
3984 AGCGCT
ApoI 1816 GAATTT [GA]^AATT[CT]
4224 GAATTC
Asp718I 2051 GGTACC G^GTACC
2245 GGTACC
BlpI 1245 GCTAAGC GC^T[ACGT]AGC
3220 GCTGAGC
Bpu1102I 1245 GCTAAGC GC^T[ACGT]AGC
3220 GCTGAGC
BsaWI 2054 ACCGGA [AT]^CCGG[AT]
3893 ACCGGT
BsiEI 73 CGGCCG CG[GA][CT]^CG
3189 CGGCCG
BsrBI 1570 CCGCTC CCGCTC(-3/-3)
910 GAGCGG
BstYI 1728 GGATCT [GA]^GATC[CT]
3405 GGATCT
Bsu36I 3900 CCTCAGG CC^T[ACGT]AGG
4157 CCTAAGG
CelII 1245 GCTAAGC GC^T[ACGT]AGC
3220 GCTGAGC
EagI 73 CGGCCG C^GGCCG
3189 CGGCCG
EclXI 73 CGGCCG C^GGCCG
3189 CGGCCG
Eco47III 3224 AGCGCT AGC^GCT
3984 AGCGCT
Eco52I 73 CGGCCG C^GGCCG
3189 CGGCCG
Eco81I 3900 CCTCAGG CC^T[ACGT]AGG
4157 CCTAAGG
KpnI 2051 GGTACC GGTAC^C
2245 GGTACC
KspI 19 CCGCGG CCGC^GG
629 CCGCGG
MflI 1728 GGATCT [GA]^GATC[CT]
3405 GGATCT
PaeR7I 573 CTCGAG C^TCGAG
1454 CTCGAG
SacII 19 CCGCGG CCGC^GG
629 CCGCGG
SbfI 2670 CCTGCAGG CCTGCA^GG
3399 CCTGCAGG
SexAI 3752 ACCTGGT A^CC[AT]GGT
3814 ACCAGGT
Sse8387I 2670 CCTGCAGG CCTGCA^GG
3399 CCTGCAGG
TfiI 3969 GATTC G^A[AT]TC
4198 GATTC
XhoI 573 CTCGAG C^TCGAG
1454 CTCGAG
XhoII 1728 GGATCT [GA]^GATC[CT]
3405 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI