##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI AscI Asp700I Asp718I
AspEI AviII BbeI BbrPI BfrI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NarI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1453 GACGTC GACGT^C
AccII 80 CGCG CG^CG
Alw44I 4479 GTGCAC G^TGCAC
AocI 3269 CCTCAGG CC^T[ACGT]AGG
ApaLI 4479 GTGCAC G^TGCAC
AseI 3928 ATTAAT AT^TAAT
AsnI 3928 ATTAAT AT^TAAT
AspI 2904 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 155 CCTAGG C^CTAGG
BamHI 2506 GGATCC G^GATCC
BcgI 2228 GCAGCTAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4430 GTATCC GTATCC(6/5)
BclI 1839 TGATCA T^GATCA
BglII 862 AGATCT A^GATCT
BlnI 155 CCTAGG C^CTAGG
BsaAI 3885 TACGTG [CT]AC^GT[GA]
BsgI 594 GTGCAG GTGCAG(16/14)
BsmBI 2298 GAGACG CGTCTC(1/5)
Bsp1407I 3889 TGTACA T^GTACA
BspHI 2443 TCATGA T^CATGA
BsrFI 787 GCCGGC [GA]^CCGG[CT]
BsrGI 3889 TGTACA T^GTACA
BstUI 80 CGCG CG^CG
Bsu36I 3269 CCTCAGG CC^T[ACGT]AGG
Cfr10I 787 GCCGGC [GA]^CCGG[CT]
DraIII 196 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 893 GAAGAG CTCTTC(1/4)
Eco81I 3269 CCTCAGG CC^T[ACGT]AGG
EcoRI 3680 GAATTC G^AATTC
FbaI 1839 TGATCA T^GATCA
HincII 4158 GTTGAC GT[CT]^[GA]AC
HindII 4158 GTTGAC GT[CT]^[GA]AC
HindIII 2342 AAGCTT A^AGCTT
Ksp632I 893 GAAGAG CTCTTC(1/4)
MvnI 80 CGCG CG^CG
NaeI 787 GCCGGC GCC^GGC
NdeI 2919 CATATG CA^TATG
NgoMIV 787 GCCGGC G^CCGGC
PflFI 2904 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2398 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3928 ATTAAT AT^TAAT
RcaI 2443 TCATGA T^CATGA
SapI 893 GAAGAGC GCTCTTC(1/4)
ScaI 584 AGTACT AGT^ACT
SspBI 3889 TGTACA T^GTACA
Tth111I 2904 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2398 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 794 GTAGAC GT^[AC][GT]AC
2327 GTAGAC
AflIII 2301 ACGTGT A^C[GA][CT]GT
3886 ACGTGT
AvaI 2375 CTCGGG C^[CT]CG[GA]G
2454 CCCGAG
BbsI 476 GAAGAC GAAGAC(2/6)
3603 GAAGAC
BglI 1174 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3250 GCCTGCTGGGC
BpuAI 476 GAAGAC GAAGAC(2/6)
3603 GAAGAC
BsoBI 2375 CTCGGG C^[CT]CG[GA]G
2454 CCCGAG
BsrBI 2532 CCGCTC CCGCTC(-3/-3)
91 GAGCGG
BssSI 388 CTCGTG CACGAG(-5/-1)
4486 CTCGTG
BstAPI 2125 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2711 GCACTTGATGC
DraI 3715 TTTAAA TTT^AAA
4515 TTTAAA
EcoNI 1274 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3323 CCTCCAGCAGG
EcoT22I 2614 ATGCAT ATGCA^T
4011 ATGCAT
HaeII 116 GGCGCT [GA]GCGC^[CT]
2488 AGCGCC
NcoI 333 CCATGG C^CATGG
3747 CCATGG
NheI 2231 GCTAGC G^CTAGC
4266 GCTAGC
NsiI 2614 ATGCAT ATGCA^T
4011 ATGCAT
PpuMI 606 AGGTCCC [GA]G^G[AT]CC[CT]
1936 GGGTCCC
SphI 315 GCATGC GCATG^C
3650 GCATGC
XcmI 1169 CCAGTGCCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3597 CCAGATGAAGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII