##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI BamHI BclI
BfrI BsaBI BseAI BsiWI BspDI
BspEI BspHI BsrDI BstBI ClaI
CpoI DraI DrdI Eam1105I EcoRV
FbaI HindIII KpnI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI ScaI SfuI SnaBI
SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3400 GTATAC GT^[AC][GT]AC
AcsI 3556 GAATTC [GA]^AATT[CT]
AgeI 2890 ACCGGT A^CCGGT
ApoI 3556 GAATTC [GA]^AATT[CT]
AspI 3491 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2449 TGCGCA TGC^GCA
AvrII 4599 CCTAGG C^CTAGG
BglII 598 AGATCT A^GATCT
BlnI 4599 CCTAGG C^CTAGG
BsmBI 401 GAGACG CGTCTC(1/5)
Bsp1407I 5002 TGTACA T^GTACA
BsrGI 5002 TGTACA T^GTACA
Bst1107I 3400 GTATAC GTA^TAC
BstZ17I 3400 GTATAC GTA^TAC
EcoRI 3556 GAATTC G^AATTC
EcoT22I 4938 ATGCAT ATGCA^T
FseI 2812 GGCCGGCC GGCCGG^CC
FspI 2449 TGCGCA TGC^GCA
NsiI 4938 ATGCAT ATGCA^T
PaeR7I 76 CTCGAG C^TCGAG
PflFI 3491 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2890 ACCGGT A^CCGGT
SbfI 4154 CCTGCAGG CCTGCA^GG
SexAI 2223 ACCTGGT A^CC[AT]GGT
SgrAI 2889 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 4154 CCTGCAGG CCTGCA^GG
SspBI 5002 TGTACA T^GTACA
TfiI 1747 GATTC G^A[AT]TC
Tth111I 3491 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 601 TCTAGA T^CTAGA
XhoI 76 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Aor51HI 613 AGCGCT AGC^GCT
883 AGCGCT
Asp700I 1051 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1602 GAATGTTTTC
BbrPI 1564 CACGTG CAC^GTG
2923 CACGTG
BbsI 1347 GTCTTC GAAGAC(2/6)
2090 GTCTTC
BcgI 1 GCACCGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3728 GCAGCGGCTTCG
BciVI 1451 GGATAC GTATCC(6/5)
3415 GGATAC
BlpI 4179 GCTGAGC GC^T[ACGT]AGC
4233 GCTGAGC
Bpu1102I 4179 GCTGAGC GC^T[ACGT]AGC
4233 GCTGAGC
BpuAI 1347 GTCTTC GAAGAC(2/6)
2090 GTCTTC
BsaAI 1564 CACGTG [CT]AC^GT[GA]
2923 CACGTG
BspLU11I 3186 ACATGT A^CATGT
3246 ACATGT
BssHII 85 GCGCGC G^CGCGC
442 GCGCGC
BssSI 1937 CACGAG CACGAG(-5/-1)
3111 CTCGTG
CelII 4179 GCTGAGC GC^T[ACGT]AGC
4233 GCTGAGC
DraIII 4162 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
4995 CACTGTGTG
Eco47III 613 AGCGCT AGC^GCT
883 AGCGCT
HincII 2479 GTTAAC GT[CT]^[GA]AC
3547 GTTAAC
HindII 2479 GTTAAC GT[CT]^[GA]AC
3547 GTTAAC
HpaI 2479 GTTAAC GTT^AAC
3547 GTTAAC
KspI 43 CCGCGG CCGC^GG
217 CCGCGG
NaeI 956 GCCGGC GCC^GGC
2813 GCCGGC
NcoI 3411 CCATGG C^CATGG
3598 CCATGG
NgoMIV 956 GCCGGC G^CCGGC
2813 GCCGGC
PmaCI 1564 CACGTG CAC^GTG
2923 CACGTG
PmlI 1564 CACGTG CAC^GTG
2923 CACGTG
PshAI 2424 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3870 GACAGCTGTC
SacII 43 CCGCGG CCGC^GG
217 CCGCGG
SmlI 76 CTCGAG C^T[CT][GA]AG
4954 CTCAAG
SphI 1923 GCATGC GCATG^C
4184 GCATGC
Tsp509I 3557 AATT ^AATT
3899 AATT
XmnI 1051 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1602 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
StuI StyI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI