##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccI AclI AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AviII BamHI BclI BlpI
Bpu1102I BsiWI BspDI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV FbaI
FseI FspI HpaI MluI NaeI
NdeI NgoMIV NotI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I RsrII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2282 GGTACC G^GTACC
Asp700I 4021 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2282 GGTACC G^GTACC
AspI 4688 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2022 CCTAGG C^CTAGG
BglI 1970 GCCTCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1655 AGATCT A^GATCT
BlnI 2022 CCTAGG C^CTAGG
BsaBI 3231 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 1047 TCATGA T^CATGA
BsrBI 1073 CCGCTC CCGCTC(-3/-3)
BsrDI 3365 GCAATG GCAATG(2/0)
BsrFI 3470 ACCGGC [GA]^CCGG[CT]
BssHII 1769 GCGCGC G^CGCGC
BstAPI 662 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 3470 ACCGGC [GA]^CCGG[CT]
DraIII 663 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 4136 GAATTC G^AATTC
EcoT22I 3232 ATGCAT ATGCA^T
HgaI 818 GCGTC GACGC(5/10)
HincII 4079 GTCAAC GT[CT]^[GA]AC
HindII 4079 GTCAAC GT[CT]^[GA]AC
HindIII 1831 AAGCTT A^AGCTT
KpnI 2282 GGTACC GGTAC^C
KspI 972 CCGCGG CCGC^GG
MamI 3231 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2127 CAATTG C^AATTG
MunI 2127 CAATTG C^AATTG
NheI 394 GCTAGC G^CTAGC
NruI 934 TCGCGA TCG^CGA
NsiI 3232 ATGCAT ATGCA^T
PflFI 4688 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4324 CCAACTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 911 CTGCAG CTGCA^G
PvuI 1357 CGATCG CGAT^CG
RcaI 1047 TCATGA T^CATGA
SacII 972 CCGCGG CCGC^GG
ScaI 2067 AGTACT AGT^ACT
SexAI 4689 ACCTGGT A^CC[AT]GGT
SmaI 3402 CCCGGG CCC^GGG
SphI 3455 GCATGC GCATG^C
StuI 3649 AGGCCT AGG^CCT
SwaI 4150 ATTTAAAT ATTT^AAAT
Tth111I 4688 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4324 CCAACTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3402 CCCGGG C^CCGGG
XmaI 3402 CCCGGG C^CCGGG
XmnI 4021 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 836 TCCGGA T^CCGGA
1152 TCCGGA
AhdI 799 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4432 GACCCAAAGTC
AocI 2044 CCTGAGG CC^T[ACGT]AGG
3541 CCTCAGG
AspEI 799 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4432 GACCCAAAGTC
BbrPI 1508 CACGTG CAC^GTG
1559 CACGTG
BciVI 73 GGATAC GTATCC(6/5)
441 GGATAC
BsaWI 836 TCCGGA [AT]^CCGG[AT]
1152 TCCGGA
BseAI 836 TCCGGA T^CCGGA
1152 TCCGGA
BsiEI 1357 CGATCG CG[GA][CT]^CG
3468 CGACCG
BsmBI 556 GAGACG CGTCTC(1/5)
1574 GAGACG
Bsp1407I 3040 TGTACA T^GTACA
4002 TGTACA
BspEI 836 TCCGGA T^CCGGA
1152 TCCGGA
BspLU11I 2242 ACATGT A^CATGT
4064 ACATGT
BsrGI 3040 TGTACA T^GTACA
4002 TGTACA
Bsu36I 2044 CCTGAGG CC^T[ACGT]AGG
3541 CCTCAGG
Eam1105I 799 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4432 GACCCAAAGTC
Eco81I 2044 CCTGAGG CC^T[ACGT]AGG
3541 CCTCAGG
MroI 836 TCCGGA T^CCGGA
1152 TCCGGA
MslI 2243 CATGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4617 CATGGGGGTG
NcoI 4375 CCATGG C^CATGG
4907 CCATGG
PmaCI 1508 CACGTG CAC^GTG
1559 CACGTG
PmlI 1508 CACGTG CAC^GTG
1559 CACGTG
PvuII 2559 CAGCTG CAG^CTG
3036 CAGCTG
SacI 923 GAGCTC GAGCT^C
4894 GAGCTC
SspBI 3040 TGTACA T^GTACA
4002 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcgI BcnI BfaI BfrI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII