################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AspI AviII BamHI BanI BbeI BbrPI BcgI BcnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI CelII CfoI Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I FauI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MroI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI Sse8387I SspBI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 4838 CGCG CG^CG AflII 149 CTTAAG C^TTAAG AseI 4767 ATTAAT AT^TAAT AsnI 4767 ATTAAT AT^TAAT AvrII 3796 CCTAGG C^CTAGG BalI 3583 TGGCCA TGG^CCA BbsI 4930 GAAGAC GAAGAC(2/6) BciVI 2281 GGATAC GTATCC(6/5) BclI 4297 TGATCA T^GATCA BfrI 149 CTTAAG C^TTAAG BglI 3894 GCCATGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 3796 CCTAGG C^CTAGG BpuAI 4930 GAAGAC GAAGAC(2/6) BstEII 1946 GGTTACC G^GT[ACGT]ACC BstPI 1946 GGTTACC G^GT[ACGT]ACC BstUI 4838 CGCG CG^CG EaeI 3583 TGGCCA [CT]^GGCC[GA] EcoNI 4295 CCTGATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1946 GGTTACC G^GT[ACGT]ACC FbaI 4297 TGATCA T^GATCA HapII 3001 CCGG C^CGG HgaI 4839 GCGTC GACGC(5/10) HpaII 3001 CCGG C^CGG MluI 4837 ACGCGT A^CGCGT MluNI 3583 TGGCCA TGG^CCA MscI 3583 TGGCCA TGG^CCA MspI 3001 CCGG C^CGG MvnI 4838 CGCG CG^CG NdeI 3723 CATATG CA^TATG PflMI 615 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 5167 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4767 ATTAAT AT^TAAT PstI 1583 CTGCAG CTGCA^G SapI 4307 GCTCTTC GCTCTTC(1/4) SnaBI 2297 TACGTA TAC^GTA SpeI 949 ACTAGT A^CTAGT SphI 4128 GCATGC GCATG^C TaqI 3100 TCGA T^CGA TthHB8I 3100 TCGA T^CGA Van91I 615 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1815 CCAAATTGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 3718 GTATAC GT^[AC][GT]AC 3744 GTATAC AocI 2960 CCTCAGG CC^T[ACGT]AGG 3258 CCTGAGG AvaI 553 CCCGAG C^[CT]CG[GA]G 3527 CTCGGG BanII 190 GAGCTC G[GA]GC[CT]^C 4082 GAGCTC BbvCI 2023 CCTCAGC CCTCAGC(-5/-2) 378 GCTGAGG BglII 2227 AGATCT A^GATCT 2755 AGATCT BsgI 627 GTGCAG GTGCAG(16/14) 1034 GTGCAG BsoBI 553 CCCGAG C^[CT]CG[GA]G 3527 CTCGGG BspHI 1504 TCATGA T^CATGA 5136 TCATGA BspLU11I 2815 ACATGT A^CATGT 3570 ACATGT Bst1107I 3718 GTATAC GTA^TAC 3744 GTATAC BstXI 1260 CCATTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4908 CCAATATAATGG BstZ17I 3718 GTATAC GTA^TAC 3744 GTATAC Bsu36I 2960 CCTCAGG CC^T[ACGT]AGG 3258 CCTGAGG DraII 2828 AGGACCC [GA]G^G[ACGT]CC[CT] 3398 GGGACCC DrdI 2071 GACAACTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2830 GACCCCAATGTC Eco81I 2960 CCTCAGG CC^T[ACGT]AGG 3258 CCTGAGG EcoO109I 2828 AGGACCC [GA]G^G[ACGT]CC[CT] 3398 GGGACCC EcoRI 803 GAATTC G^AATTC 2238 GAATTC EcoRV 1927 GATATC GAT^ATC 4752 GATATC HincII 3119 GTCAAC GT[CT]^[GA]AC 5089 GTTGAC HindII 3119 GTCAAC GT[CT]^[GA]AC 5089 GTTGAC HindIII 2119 AAGCTT A^AGCTT 2567 AAGCTT MfeI 2364 CAATTG C^AATTG 4621 CAATTG MunI 2364 CAATTG C^AATTG 4621 CAATTG PpuMI 2828 AGGACCC [GA]G^G[AT]CC[CT] 3398 GGGACCC RcaI 1504 TCATGA T^CATGA 5136 TCATGA SacI 190 GAGCTC GAGCT^C 4082 GAGCTC SfcI 565 CTACAG C^T[GA][CT]AG 1583 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaII BbvI BfaI BmyI BpmI BsaAI BsaBI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DsaI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI SspI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI XhoII XmnI