##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII BamHI
BanI BbeI BbrPI BcgI BcnI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI CelII
CfoI Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
FauI FseI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MroI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
Sse8387I SspBI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 4838 CGCG CG^CG
AflII 149 CTTAAG C^TTAAG
AseI 4767 ATTAAT AT^TAAT
AsnI 4767 ATTAAT AT^TAAT
AvrII 3796 CCTAGG C^CTAGG
BalI 3583 TGGCCA TGG^CCA
BbsI 4930 GAAGAC GAAGAC(2/6)
BciVI 2281 GGATAC GTATCC(6/5)
BclI 4297 TGATCA T^GATCA
BfrI 149 CTTAAG C^TTAAG
BglI 3894 GCCATGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3796 CCTAGG C^CTAGG
BpuAI 4930 GAAGAC GAAGAC(2/6)
BstEII 1946 GGTTACC G^GT[ACGT]ACC
BstPI 1946 GGTTACC G^GT[ACGT]ACC
BstUI 4838 CGCG CG^CG
EaeI 3583 TGGCCA [CT]^GGCC[GA]
EcoNI 4295 CCTGATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1946 GGTTACC G^GT[ACGT]ACC
FbaI 4297 TGATCA T^GATCA
HapII 3001 CCGG C^CGG
HgaI 4839 GCGTC GACGC(5/10)
HpaII 3001 CCGG C^CGG
MluI 4837 ACGCGT A^CGCGT
MluNI 3583 TGGCCA TGG^CCA
MscI 3583 TGGCCA TGG^CCA
MspI 3001 CCGG C^CGG
MvnI 4838 CGCG CG^CG
NdeI 3723 CATATG CA^TATG
PflMI 615 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 5167 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4767 ATTAAT AT^TAAT
PstI 1583 CTGCAG CTGCA^G
SapI 4307 GCTCTTC GCTCTTC(1/4)
SnaBI 2297 TACGTA TAC^GTA
SpeI 949 ACTAGT A^CTAGT
SphI 4128 GCATGC GCATG^C
TaqI 3100 TCGA T^CGA
TthHB8I 3100 TCGA T^CGA
Van91I 615 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1815 CCAAATTGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 3718 GTATAC GT^[AC][GT]AC
3744 GTATAC
AocI 2960 CCTCAGG CC^T[ACGT]AGG
3258 CCTGAGG
AvaI 553 CCCGAG C^[CT]CG[GA]G
3527 CTCGGG
BanII 190 GAGCTC G[GA]GC[CT]^C
4082 GAGCTC
BbvCI 2023 CCTCAGC CCTCAGC(-5/-2)
378 GCTGAGG
BglII 2227 AGATCT A^GATCT
2755 AGATCT
BsgI 627 GTGCAG GTGCAG(16/14)
1034 GTGCAG
BsoBI 553 CCCGAG C^[CT]CG[GA]G
3527 CTCGGG
BspHI 1504 TCATGA T^CATGA
5136 TCATGA
BspLU11I 2815 ACATGT A^CATGT
3570 ACATGT
Bst1107I 3718 GTATAC GTA^TAC
3744 GTATAC
BstXI 1260 CCATTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4908 CCAATATAATGG
BstZ17I 3718 GTATAC GTA^TAC
3744 GTATAC
Bsu36I 2960 CCTCAGG CC^T[ACGT]AGG
3258 CCTGAGG
DraII 2828 AGGACCC [GA]G^G[ACGT]CC[CT]
3398 GGGACCC
DrdI 2071 GACAACTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2830 GACCCCAATGTC
Eco81I 2960 CCTCAGG CC^T[ACGT]AGG
3258 CCTGAGG
EcoO109I 2828 AGGACCC [GA]G^G[ACGT]CC[CT]
3398 GGGACCC
EcoRI 803 GAATTC G^AATTC
2238 GAATTC
EcoRV 1927 GATATC GAT^ATC
4752 GATATC
HincII 3119 GTCAAC GT[CT]^[GA]AC
5089 GTTGAC
HindII 3119 GTCAAC GT[CT]^[GA]AC
5089 GTTGAC
HindIII 2119 AAGCTT A^AGCTT
2567 AAGCTT
MfeI 2364 CAATTG C^AATTG
4621 CAATTG
MunI 2364 CAATTG C^AATTG
4621 CAATTG
PpuMI 2828 AGGACCC [GA]G^G[AT]CC[CT]
3398 GGGACCC
RcaI 1504 TCATGA T^CATGA
5136 TCATGA
SacI 190 GAGCTC GAGCT^C
4082 GAGCTC
SfcI 565 CTACAG C^T[GA][CT]AG
1583 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AvaII BbvI BfaI
BmyI BpmI BsaAI BsaBI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI SspI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XbaI XhoII
XmnI