##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AocI Aor51HI AscI Asp718I AviII
AvrII BalI BbrPI BcgI BglI
BlnI BsaI BsaWI BseAI BsiWI
BsmBI BspEI BspMI BssHII BssSI
BstAPI BstBI Bsu36I CpoI DraIII
DrdI Eco47III Eco81I EcoRI EcoRV
FseI FspI HpaI KpnI KspI
MluI MluNI MroI MscI NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI Sse8387I Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 2024 GTATAC GT^[AC][GT]AC
AhdI 472 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3428 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 472 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1511 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1390 GGATCC G^GATCC
BbeI 70 GGCGCC GGCGC^C
BbsI 799 GAAGAC GAAGAC(2/6)
BclI 1108 TGATCA T^GATCA
BpuAI 799 GAAGAC GAAGAC(2/6)
BsaAI 2720 CACGTA [CT]AC^GT[GA]
BsgI 51 CTGCAC GTGCAG(16/14)
BspDI 1758 ATCGAT AT^CGAT
BsrFI 10 GCCGGC [GA]^CCGG[CT]
Bst1107I 2024 GTATAC GTA^TAC
BstEII 982 GGTGACC G^GT[ACGT]ACC
BstPI 982 GGTGACC G^GT[ACGT]ACC
BstZ17I 2024 GTATAC GTA^TAC
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
ClaI 1758 ATCGAT AT^CGAT
DsaI 213 CCATGG C^C[GA][CT]GG
Eam1105I 472 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 982 GGTGACC G^GT[ACGT]ACC
EcoT14I 213 CCATGG C^C[AT][AT]GG
FbaI 1108 TGATCA T^GATCA
HgaI 542 GCGTC GACGC(5/10)
HindIII 4588 AAGCTT A^AGCTT
KasI 70 GGCGCC G^GCGCC
MfeI 2997 CAATTG C^AATTG
MunI 2997 CAATTG C^AATTG
NaeI 10 GCCGGC GCC^GGC
NarI 70 GGCGCC GG^CGCC
NcoI 213 CCATGG C^CATGG
NgoMIV 10 GCCGGC G^CCGGC
PflFI 1511 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1823 AGGACCT [GA]G^G[AT]CC[CT]
SapI 933 GCTCTTC GCTCTTC(1/4)
SexAI 1512 ACCAGGT A^CC[AT]GGT
SfoI 70 GGCGCC GGC^GCC
SphI 1047 GCATGC GCATG^C
StyI 213 CCATGG C^C[AT][AT]GG
SwaI 1098 ATTTAAAT ATTT^AAAT
Tth111I 1511 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 900 CCAAGCCCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 70 GGCGCC G[GA]^CG[CT]C
541 GGCGTC
ApaI 63 GGGCCC GGGCC^C
97 GGGCCC
AvaI 66 CCCGGG C^[CT]CG[GA]G
124 CCCGGG
AvaII 1824 GGACC G^G[AT]CC
2452 GGTCC
BanI 70 GGCGCC G^G[CT][GA]CC
209 GGCACC
BbvCI 140 GCTGAGG CCTCAGC(-5/-2)
4556 GCTGAGG
BciVI 1000 GGATAC GTATCC(6/5)
2704 GTATCC
BsaBI 2345 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3425 GATCAGCATC
BsaHI 70 GGCGCC G[GA]^CG[CT]C
541 GGCGTC
BsiEI 12 CGGCCG CG[GA][CT]^CG
193 CGGCCG
BsmAI 2391 GAGAC GTCTC(1/5)
1378 GTCTC
BsoBI 66 CCCGGG C^[CT]CG[GA]G
124 CCCGGG
BsrBI 4 CCGCTC CCGCTC(-3/-3)
80 CCGCTC
EaeI 12 CGGCCG [CT]^GGCC[GA]
193 CGGCCG
EagI 12 CGGCCG C^GGCCG
193 CGGCCG
EclXI 12 CGGCCG C^GGCCG
193 CGGCCG
Eco52I 12 CGGCCG C^GGCCG
193 CGGCCG
Hin1I 70 GGCGCC G[GA]^CG[CT]C
541 GGCGTC
MamI 2345 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3425 GATCAGCATC
PleI 3262 GAGTC GAGTC(4/5)
4294 GAGTC
SacI 990 GAGCTC GAGCT^C
1925 GAGCTC
SmaI 66 CCCGGG CCC^GGG
124 CCCGGG
SpeI 3078 ACTAGT A^CTAGT
3689 ACTAGT
StuI 1854 AGGCCT AGG^CCT
1954 AGGCCT
XmaCI 66 CCCGGG C^CCGGG
124 CCCGGG
XmaI 66 CCCGGG C^CCGGG
124 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI Alw44I AlwI ApaLI
ApoI AseI AsnI Asp700I AspHI
BanII BbvI BcnI BfaI BfrI
BglII BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspLU11I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII EarI EcoNI
EcoO109I EcoRII EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NsiI NspI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI