##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AflII AscI AseI AsnI AvrII
BamHI BbvCI BciVI BclI BfrI
BglII BlnI BsaBI BsiWI BspDI
BsrBI BssSI Bst1107I BstYI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoRI EcoRV FbaI FseI HincII
HindII HpaI MamI MfeI MflI
MluI MunI NdeI NheI NotI
PacI PmeI PshAI PshBI RsrII
SalI SgrAI SnaBI SpeI SspI
SwaI XhoII
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 3280 GTAGAC GT^[AC][GT]AC
AccIII 3989 TCCGGA T^CCGGA
AclI 648 AACGTT AA^CGTT
AgeI 2208 ACCGGT A^CCGGT
AlwI 1250 GATCC GGATC(4/5)
Aor51HI 1673 AGCGCT AGC^GCT
AspI 1040 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1755 GGCGCC GGCGC^C
BbrPI 2948 CACGTG CAC^GTG
BcgI 2391 GCACCACGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 3989 TCCGGA T^CCGGA
BsmI 2832 GAATGC GAATGC(1/-1)
Bsp1407I 2306 TGTACA T^GTACA
BspEI 3989 TCCGGA T^CCGGA
BsrGI 2306 TGTACA T^GTACA
BstBI 682 TTCGAA TT^CGAA
BstEII 2204 GGTGACC G^GT[ACGT]ACC
BstPI 2204 GGTGACC G^GT[ACGT]ACC
DraI 798 TTTAAA TTT^AAA
DraIII 4460 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1673 AGCGCT AGC^GCT
EcoO65I 2204 GGTGACC G^GT[ACGT]ACC
EcoT22I 636 ATGCAT ATGCA^T
HindIII 803 AAGCTT A^AGCTT
KasI 1755 GGCGCC G^GCGCC
KspI 1661 CCGCGG CCGC^GG
MroI 3989 TCCGGA T^CCGGA
NarI 1755 GGCGCC GG^CGCC
NcoI 1763 CCATGG C^CATGG
NruI 4017 TCGCGA TCG^CGA
NsiI 636 ATGCAT ATGCA^T
NspV 682 TTCGAA TT^CGAA
PaeR7I 2887 CTCGAG C^TCGAG
PflFI 1040 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2208 ACCGGT A^CCGGT
PmaCI 2948 CACGTG CAC^GTG
PmlI 2948 CACGTG CAC^GTG
Psp1406I 648 AACGTT AA^CGTT
PvuI 2397 CGATCG CGAT^CG
SacII 1661 CCGCGG CCGC^GG
SapI 1272 GAAGAGC GCTCTTC(1/4)
ScaI 155 AGTACT AGT^ACT
SexAI 248 ACCTGGT A^CC[AT]GGT
SfoI 1755 GGCGCC GGC^GCC
SfuI 682 TTCGAA TT^CGAA
SspBI 2306 TGTACA T^GTACA
Tth111I 1040 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5005 TCTAGA T^CTAGA
XhoI 2887 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 3664 GACGTC GACGT^C
4436 GACGTC
Acc65I 2081 GGTACC G^GTACC
2853 GGTACC
AcsI 764 GAATTT [GA]^AATT[CT]
4746 GAATTT
AhdI 2533 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2946 GACACGTGGTC
ApoI 764 GAATTT [GA]^AATT[CT]
4746 GAATTT
Asp718I 2081 GGTACC G^GTACC
2853 GGTACC
AspEI 2533 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2946 GACACGTGGTC
BbsI 1389 GAAGAC GAAGAC(2/6)
1607 GAAGAC
BpuAI 1389 GAAGAC GAAGAC(2/6)
1607 GAAGAC
BsaWI 2208 ACCGGT [AT]^CCGG[AT]
3989 TCCGGA
BsiEI 1932 CGACCG CG[GA][CT]^CG
2397 CGATCG
BspLU11I 4536 ACATGT A^CATGT
4794 ACATGT
BsrDI 633 GCAATG GCAATG(2/0)
2696 GCAATG
BssHII 1223 GCGCGC G^CGCGC
1593 GCGCGC
DrdI 1996 GACCCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3551 GACAGGGCGGTC
Eam1105I 2533 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2946 GACACGTGGTC
KpnI 2081 GGTACC GGTAC^C
2853 GGTACC
PflMI 1613 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1763 CCATGGGGTGG
SacI 2548 GAGCTC GAGCT^C
3490 GAGCTC
SbfI 3442 CCTGCAGG CCTGCA^GG
5085 CCTGCAGG
SfiI 1709 GGCCCGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1718 GGCCGTGCTGGCC
SmlI 411 CTCAAG C^T[CT][GA]AG
2887 CTCGAG
SphI 1741 GCATGC GCATG^C
3903 GCATGC
Sse8387I 3442 CCTGCAGG CCTGCA^GG
5085 CCTGCAGG
StuI 2822 AGGCCT AGG^CCT
4233 AGGCCT
Van91I 1613 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1763 CCATGGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwNI AocI ApaI
ApaLI Asp700I AspHI AvaI AvaII
AviII BalI BanI BanII BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmaCI XmaI
XmnI