################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AflII AscI AseI AsnI AvrII BamHI BbvCI BciVI BclI BfrI BglII BlnI BsaBI BsiWI BspDI BsrBI BssSI Bst1107I BstYI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoRI EcoRV FbaI FseI HincII HindII HpaI MamI MfeI MflI MluI MunI NdeI NheI NotI PacI PmeI PshAI PshBI RsrII SalI SgrAI SnaBI SpeI SspI SwaI XhoII ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 3280 GTAGAC GT^[AC][GT]AC AccIII 3989 TCCGGA T^CCGGA AclI 648 AACGTT AA^CGTT AgeI 2208 ACCGGT A^CCGGT AlwI 1250 GATCC GGATC(4/5) Aor51HI 1673 AGCGCT AGC^GCT AspI 1040 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 1755 GGCGCC GGCGC^C BbrPI 2948 CACGTG CAC^GTG BcgI 2391 GCACCACGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BseAI 3989 TCCGGA T^CCGGA BsmI 2832 GAATGC GAATGC(1/-1) Bsp1407I 2306 TGTACA T^GTACA BspEI 3989 TCCGGA T^CCGGA BsrGI 2306 TGTACA T^GTACA BstBI 682 TTCGAA TT^CGAA BstEII 2204 GGTGACC G^GT[ACGT]ACC BstPI 2204 GGTGACC G^GT[ACGT]ACC DraI 798 TTTAAA TTT^AAA DraIII 4460 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1673 AGCGCT AGC^GCT EcoO65I 2204 GGTGACC G^GT[ACGT]ACC EcoT22I 636 ATGCAT ATGCA^T HindIII 803 AAGCTT A^AGCTT KasI 1755 GGCGCC G^GCGCC KspI 1661 CCGCGG CCGC^GG MroI 3989 TCCGGA T^CCGGA NarI 1755 GGCGCC GG^CGCC NcoI 1763 CCATGG C^CATGG NruI 4017 TCGCGA TCG^CGA NsiI 636 ATGCAT ATGCA^T NspV 682 TTCGAA TT^CGAA PaeR7I 2887 CTCGAG C^TCGAG PflFI 1040 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2208 ACCGGT A^CCGGT PmaCI 2948 CACGTG CAC^GTG PmlI 2948 CACGTG CAC^GTG Psp1406I 648 AACGTT AA^CGTT PvuI 2397 CGATCG CGAT^CG SacII 1661 CCGCGG CCGC^GG SapI 1272 GAAGAGC GCTCTTC(1/4) ScaI 155 AGTACT AGT^ACT SexAI 248 ACCTGGT A^CC[AT]GGT SfoI 1755 GGCGCC GGC^GCC SfuI 682 TTCGAA TT^CGAA SspBI 2306 TGTACA T^GTACA Tth111I 1040 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5005 TCTAGA T^CTAGA XhoI 2887 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AatII 3664 GACGTC GACGT^C 4436 GACGTC Acc65I 2081 GGTACC G^GTACC 2853 GGTACC AcsI 764 GAATTT [GA]^AATT[CT] 4746 GAATTT AhdI 2533 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2946 GACACGTGGTC ApoI 764 GAATTT [GA]^AATT[CT] 4746 GAATTT Asp718I 2081 GGTACC G^GTACC 2853 GGTACC AspEI 2533 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2946 GACACGTGGTC BbsI 1389 GAAGAC GAAGAC(2/6) 1607 GAAGAC BpuAI 1389 GAAGAC GAAGAC(2/6) 1607 GAAGAC BsaWI 2208 ACCGGT [AT]^CCGG[AT] 3989 TCCGGA BsiEI 1932 CGACCG CG[GA][CT]^CG 2397 CGATCG BspLU11I 4536 ACATGT A^CATGT 4794 ACATGT BsrDI 633 GCAATG GCAATG(2/0) 2696 GCAATG BssHII 1223 GCGCGC G^CGCGC 1593 GCGCGC DrdI 1996 GACCCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3551 GACAGGGCGGTC Eam1105I 2533 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2946 GACACGTGGTC KpnI 2081 GGTACC GGTAC^C 2853 GGTACC PflMI 1613 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1763 CCATGGGGTGG SacI 2548 GAGCTC GAGCT^C 3490 GAGCTC SbfI 3442 CCTGCAGG CCTGCA^GG 5085 CCTGCAGG SfiI 1709 GGCCCGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1718 GGCCGTGCTGGCC SmlI 411 CTCAAG C^T[CT][GA]AG 2887 CTCGAG SphI 1741 GCATGC GCATG^C 3903 GCATGC Sse8387I 3442 CCTGCAGG CCTGCA^GG 5085 CCTGCAGG StuI 2822 AGGCCT AGG^CCT 4233 AGGCCT Van91I 1613 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1763 CCATGGGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI Alw44I AlwNI AocI ApaI ApaLI Asp700I AspHI AvaI AvaII AviII BalI BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmaCI XmaI XmnI