##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AhdI AocI Aor51HI ApaI AscI
AspEI AviII BbeI BglI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BsrFI BssHII
BssSI BstBI BstUI Bsu36I Cfr10I
ClaI CpoI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I FseI
FspI Hin1I HindIII KasI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PflMI
PinAI PpuMI PshAI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SpeI Sse8387I
StuI SwaI Van91I
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 46 GGTACC G^GTACC
AclI 1742 AACGTT AA^CGTT
AlwNI 474 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1579 ATTAAT AT^TAAT
AsnI 1579 ATTAAT AT^TAAT
Asp718I 46 GGTACC G^GTACC
BamHI 4384 GGATCC G^GATCC
BanI 46 GGTACC G^G[CT][GA]CC
BbsI 2140 GTCTTC GAAGAC(2/6)
BciVI 1365 GTATCC GTATCC(6/5)
BpuAI 2140 GTCTTC GAAGAC(2/6)
BsaBI 3580 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 596 GAATGC GAATGC(1/-1)
BspMI 3514 GCAGGT ACCTGC(4/8)
BstAPI 996 GCATTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3698 GGTCACC G^GT[ACGT]ACC
BstPI 3698 GGTCACC G^GT[ACGT]ACC
CfoI 3080 GCGC GCG^C
DraIII 3164 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1295 GACAATCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3698 GGTCACC G^GT[ACGT]ACC
EcoRV 1564 GATATC GAT^ATC
HaeII 3079 GGCGCT [GA]GCGC^[CT]
HgaI 23 GCGTC GACGC(5/10)
HhaI 3080 GCGC GCG^C
HinP1I 3080 GCGC G^CGC
HpaI 4112 GTTAAC GTT^AAC
KpnI 46 GGTACC GGTAC^C
MamI 3580 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 3890 CATTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 4869 CAGCTG C[AC]G^C[GT]G
PaeR7I 541 CTCGAG C^TCGAG
PmeI 1201 GTTTAAAC GTTT^AAAC
PshBI 1579 ATTAAT AT^TAAT
Psp1406I 1742 AACGTT AA^CGTT
PvuII 4869 CAGCTG CAG^CTG
SapI 4601 GCTCTTC GCTCTTC(1/4)
ScaI 3875 AGTACT AGT^ACT
SmaI 4353 CCCGGG CCC^GGG
SnaBI 2296 TACGTA TAC^GTA
XcmI 960 CCACTTGTACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 541 CTCGAG C^TCGAG
XmaCI 4353 CCCGGG C^CCGGG
XmaI 4353 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 1601 GTGCAC G^TGCAC
3471 GTGCAC
ApaLI 1601 GTGCAC G^TGCAC
3471 GTGCAC
Asp700I 4625 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4688 GAACATATTC
AspI 2181 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4162 GACAAGGTC
AvrII 1394 CCTAGG C^CTAGG
4560 CCTAGG
BalI 454 TGGCCA TGG^CCA
4307 TGGCCA
BbrPI 1014 CACGTG CAC^GTG
3468 CACGTG
BcgI 3743 CGAGGAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1339 GCAGTATGGTCG
BclI 1477 TGATCA T^GATCA
4204 TGATCA
BglII 2149 AGATCT A^GATCT
2854 AGATCT
BlnI 1394 CCTAGG C^CTAGG
4560 CCTAGG
BpmI 3661 CTCCAG CTGGAG(16/14)
2677 CTGGAG
BsgI 1162 GTGCAG GTGCAG(16/14)
4193 GTGCAG
Bsp1407I 2890 TGTACA T^GTACA
4747 TGTACA
BspHI 1696 TCATGA T^CATGA
3208 TCATGA
BsrGI 2890 TGTACA T^GTACA
4747 TGTACA
Bst1107I 2566 GTATAC GTA^TAC
2829 GTATAC
BstZ17I 2566 GTATAC GTA^TAC
2829 GTATAC
DraII 2521 AGGCCCT [GA]G^G[ACGT]CC[CT]
3333 AGGCCCC
EaeI 454 TGGCCA [CT]^GGCC[GA]
4307 TGGCCA
EcoO109I 2521 AGGCCCT [GA]G^G[ACGT]CC[CT]
3333 AGGCCCC
EcoT22I 313 ATGCAT ATGCA^T
1575 ATGCAT
FauI 4007 CCCGC CCCGC(4/6)
4391 CCCGC
FbaI 1477 TGATCA T^GATCA
4204 TGATCA
HapII 3845 CCGG C^CGG
4354 CCGG
HpaII 3845 CCGG C^CGG
4354 CCGG
MluNI 454 TGGCCA TGG^CCA
4307 TGGCCA
MscI 454 TGGCCA TGG^CCA
4307 TGGCCA
MspI 3845 CCGG C^CGG
4354 CCGG
NdeI 3250 CATATG CA^TATG
4893 CATATG
NsiI 313 ATGCAT ATGCA^T
1575 ATGCAT
PflFI 2181 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4162 GACAAGGTC
PmaCI 1014 CACGTG CAC^GTG
3468 CACGTG
PmlI 1014 CACGTG CAC^GTG
3468 CACGTG
PstI 2798 CTGCAG CTGCA^G
4217 CTGCAG
RcaI 1696 TCATGA T^CATGA
3208 TCATGA
SacI 3658 GAGCTC GAGCT^C
4375 GAGCTC
SphI 194 GCATGC GCATG^C
4528 GCATGC
SspBI 2890 TGTACA T^GTACA
4747 TGTACA
Tth111I 2181 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4162 GACAAGGTC
XbaI 1151 TCTAGA T^CTAGA
4839 TCTAGA
XmnI 4625 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4688 GAACATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI ApoI AspHI
AvaI AvaII BanII BbvCI BbvI
BcnI BfaI BfrI BlpI BmyI
Bpu1102I BsaAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI EarI EcoNI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII