################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AccII AccIII AcyI AgeI AhdI AocI Aor51HI ApaI AscI AspEI AviII BbeI BglI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstBI BstUI Bsu36I Cfr10I ClaI CpoI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I FseI FspI Hin1I HindIII KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PflMI PinAI PpuMI PshAI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SpeI Sse8387I StuI SwaI Van91I ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 46 GGTACC G^GTACC AclI 1742 AACGTT AA^CGTT AlwNI 474 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 1579 ATTAAT AT^TAAT AsnI 1579 ATTAAT AT^TAAT Asp718I 46 GGTACC G^GTACC BamHI 4384 GGATCC G^GATCC BanI 46 GGTACC G^G[CT][GA]CC BbsI 2140 GTCTTC GAAGAC(2/6) BciVI 1365 GTATCC GTATCC(6/5) BpuAI 2140 GTCTTC GAAGAC(2/6) BsaBI 3580 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 596 GAATGC GAATGC(1/-1) BspMI 3514 GCAGGT ACCTGC(4/8) BstAPI 996 GCATTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3698 GGTCACC G^GT[ACGT]ACC BstPI 3698 GGTCACC G^GT[ACGT]ACC CfoI 3080 GCGC GCG^C DraIII 3164 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1295 GACAATCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3698 GGTCACC G^GT[ACGT]ACC EcoRV 1564 GATATC GAT^ATC HaeII 3079 GGCGCT [GA]GCGC^[CT] HgaI 23 GCGTC GACGC(5/10) HhaI 3080 GCGC GCG^C HinP1I 3080 GCGC G^CGC HpaI 4112 GTTAAC GTT^AAC KpnI 46 GGTACC GGTAC^C MamI 3580 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 3890 CATTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 4869 CAGCTG C[AC]G^C[GT]G PaeR7I 541 CTCGAG C^TCGAG PmeI 1201 GTTTAAAC GTTT^AAAC PshBI 1579 ATTAAT AT^TAAT Psp1406I 1742 AACGTT AA^CGTT PvuII 4869 CAGCTG CAG^CTG SapI 4601 GCTCTTC GCTCTTC(1/4) ScaI 3875 AGTACT AGT^ACT SmaI 4353 CCCGGG CCC^GGG SnaBI 2296 TACGTA TAC^GTA XcmI 960 CCACTTGTACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 541 CTCGAG C^TCGAG XmaCI 4353 CCCGGG C^CCGGG XmaI 4353 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### Alw44I 1601 GTGCAC G^TGCAC 3471 GTGCAC ApaLI 1601 GTGCAC G^TGCAC 3471 GTGCAC Asp700I 4625 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4688 GAACATATTC AspI 2181 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4162 GACAAGGTC AvrII 1394 CCTAGG C^CTAGG 4560 CCTAGG BalI 454 TGGCCA TGG^CCA 4307 TGGCCA BbrPI 1014 CACGTG CAC^GTG 3468 CACGTG BcgI 3743 CGAGGAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1339 GCAGTATGGTCG BclI 1477 TGATCA T^GATCA 4204 TGATCA BglII 2149 AGATCT A^GATCT 2854 AGATCT BlnI 1394 CCTAGG C^CTAGG 4560 CCTAGG BpmI 3661 CTCCAG CTGGAG(16/14) 2677 CTGGAG BsgI 1162 GTGCAG GTGCAG(16/14) 4193 GTGCAG Bsp1407I 2890 TGTACA T^GTACA 4747 TGTACA BspHI 1696 TCATGA T^CATGA 3208 TCATGA BsrGI 2890 TGTACA T^GTACA 4747 TGTACA Bst1107I 2566 GTATAC GTA^TAC 2829 GTATAC BstZ17I 2566 GTATAC GTA^TAC 2829 GTATAC DraII 2521 AGGCCCT [GA]G^G[ACGT]CC[CT] 3333 AGGCCCC EaeI 454 TGGCCA [CT]^GGCC[GA] 4307 TGGCCA EcoO109I 2521 AGGCCCT [GA]G^G[ACGT]CC[CT] 3333 AGGCCCC EcoT22I 313 ATGCAT ATGCA^T 1575 ATGCAT FauI 4007 CCCGC CCCGC(4/6) 4391 CCCGC FbaI 1477 TGATCA T^GATCA 4204 TGATCA HapII 3845 CCGG C^CGG 4354 CCGG HpaII 3845 CCGG C^CGG 4354 CCGG MluNI 454 TGGCCA TGG^CCA 4307 TGGCCA MscI 454 TGGCCA TGG^CCA 4307 TGGCCA MspI 3845 CCGG C^CGG 4354 CCGG NdeI 3250 CATATG CA^TATG 4893 CATATG NsiI 313 ATGCAT ATGCA^T 1575 ATGCAT PflFI 2181 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4162 GACAAGGTC PmaCI 1014 CACGTG CAC^GTG 3468 CACGTG PmlI 1014 CACGTG CAC^GTG 3468 CACGTG PstI 2798 CTGCAG CTGCA^G 4217 CTGCAG RcaI 1696 TCATGA T^CATGA 3208 TCATGA SacI 3658 GAGCTC GAGCT^C 4375 GAGCTC SphI 194 GCATGC GCATG^C 4528 GCATGC SspBI 2890 TGTACA T^GTACA 4747 TGTACA Tth111I 2181 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4162 GACAAGGTC XbaI 1151 TCTAGA T^CTAGA 4839 TCTAGA XmnI 4625 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4688 GAACATATTC ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AflIII AluI AlwI ApoI AspHI AvaI AvaII BanII BbvCI BbvI BcnI BfaI BfrI BlpI BmyI Bpu1102I BsaAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI EarI EcoNI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII