################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI Aor51HI AscI AseI AsnI Asp718I AviII BamHI BbeI BbrPI BbvCI BfrI BlpI Bpu1102I BsaWI BseAI BsgI BsiEI BsiWI BspEI BssHII Bst1107I BstAPI BstXI BstZ17I CelII CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoRV FseI FspI HaeII HpaI KasI KpnI KspI MluI MroI NarI NcoI NheI NotI NruI PacI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SexAI SfiI SfoI SgrAI SpeI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 1349 GACGTC GACGT^C AcyI 1349 GACGTC G[GA]^CG[CT]C AhdI 4751 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4343 GTGCAC G^TGCAC AocI 3879 CCTTAGG CC^T[ACGT]AGG ApaI 142 GGGCCC GGGCC^C ApaLI 4343 GTGCAC G^TGCAC AspEI 4751 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 4913 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 649 GGTGCC G^G[CT][GA]CC BbsI 3511 GAAGAC GAAGAC(2/6) BcgI 4636 GCATAACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 954 GTATCC GTATCC(6/5) BclI 1422 TGATCA T^GATCA BpuAI 3511 GAAGAC GAAGAC(2/6) BsaAI 386 TACGTA [CT]AC^GT[GA] BsaBI 3714 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1349 GACGTC G[GA]^CG[CT]C BsmBI 4839 CGTCTC CGTCTC(1/5) BspDI 4390 ATCGAT AT^CGAT BspMI 340 ACCTGC ACCTGC(4/8) BstEII 3013 GGTAACC G^GT[ACGT]ACC BstPI 3013 GGTAACC G^GT[ACGT]ACC Bsu36I 3879 CCTTAGG CC^T[ACGT]AGG ClaI 4390 ATCGAT AT^CGAT DraII 4723 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 1141 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 146 CCGTGG C^C[GA][CT]GG Eam1105I 4751 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3879 CCTTAGG CC^T[ACGT]AGG EcoNI 3003 CCTTAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 4723 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 3013 GGTAACC G^GT[ACGT]ACC FbaI 1422 TGATCA T^GATCA HgaI 4838 GCGTC GACGC(5/10) Hin1I 1349 GACGTC G[GA]^CG[CT]C HincII 2171 GTCGAC GT[CT]^[GA]AC HindII 2171 GTCGAC GT[CT]^[GA]AC MamI 3714 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3842 CAATTG C^AATTG MspA1I 1834 CAGCTG C[AC]G^C[GT]G MunI 3842 CAATTG C^AATTG NaeI 101 GCCGGC GCC^GGC NgoMIV 101 GCCGGC G^CCGGC PaeR7I 1727 CTCGAG C^TCGAG PflFI 4913 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3432 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1834 CAGCTG CAG^CTG SalI 2171 GTCGAC G^TCGAC SapI 4772 GCTCTTC GCTCTTC(1/4) SmaI 96 CCCGGG CCC^GGG SnaBI 386 TACGTA TAC^GTA StuI 2262 AGGCCT AGG^CCT Tth111I 4913 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2376 CCACATGCTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1727 CTCGAG C^TCGAG XmaCI 96 CCCGGG C^CCGGG XmaI 96 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 2171 GTCGAC GT^[AC][GT]AC 4236 GTCTAC AlwNI 868 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG 3098 CAGAACCTG AvaI 96 CCCGGG C^[CT]CG[GA]G 1727 CTCGAG AvrII 357 CCTAGG C^CTAGG 4936 CCTAGG BglI 46 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4805 GCCACTGTGGC BglII 276 AGATCT A^GATCT 4680 AGATCT BlnI 357 CCTAGG C^CTAGG 4936 CCTAGG BsoBI 96 CCCGGG C^[CT]CG[GA]G 1727 CTCGAG Bsp1407I 1697 TGTACA T^GTACA 2496 TGTACA BsrFI 101 GCCGGC [GA]^CCGG[CT] 2248 ACCGGC BsrGI 1697 TGTACA T^GTACA 2496 TGTACA BssSI 1478 CACGAG CACGAG(-5/-1) 2969 CTCGTG BstBI 287 TTCGAA TT^CGAA 4644 TTCGAA Cfr10I 101 GCCGGC [GA]^CCGG[CT] 2248 ACCGGC EcoRI 3075 GAATTC G^AATTC 3885 GAATTC EcoT22I 830 ATGCAT ATGCA^T 4310 ATGCAT NdeI 833 CATATG CA^TATG 3158 CATATG NsiI 830 ATGCAT ATGCA^T 4310 ATGCAT NspV 287 TTCGAA TT^CGAA 4644 TTCGAA PflMI 1830 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3444 CCAGGCATTGG PstI 1225 CTGCAG CTGCA^G 1615 CTGCAG SacI 2756 GAGCTC GAGCT^C 3818 GAGCTC SfuI 287 TTCGAA TT^CGAA 4644 TTCGAA SspBI 1697 TGTACA T^GTACA 2496 TGTACA SspI 4594 AATATT AAT^ATT 4998 AATATT Van91I 1830 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3444 CCAGGCATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspLU11I BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI