##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII BamHI BbeI BbrPI BbvCI
BfrI BlpI Bpu1102I BsaWI BseAI
BsgI BsiEI BsiWI BspEI BssHII
Bst1107I BstAPI BstXI BstZ17I CelII
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoRV FseI FspI
HaeII HpaI KasI KpnI KspI
MluI MroI NarI NcoI NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacII SbfI ScaI
SexAI SfiI SfoI SgrAI SpeI
Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 1349 GACGTC GACGT^C
AcyI 1349 GACGTC G[GA]^CG[CT]C
AhdI 4751 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4343 GTGCAC G^TGCAC
AocI 3879 CCTTAGG CC^T[ACGT]AGG
ApaI 142 GGGCCC GGGCC^C
ApaLI 4343 GTGCAC G^TGCAC
AspEI 4751 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4913 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 649 GGTGCC G^G[CT][GA]CC
BbsI 3511 GAAGAC GAAGAC(2/6)
BcgI 4636 GCATAACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 954 GTATCC GTATCC(6/5)
BclI 1422 TGATCA T^GATCA
BpuAI 3511 GAAGAC GAAGAC(2/6)
BsaAI 386 TACGTA [CT]AC^GT[GA]
BsaBI 3714 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1349 GACGTC G[GA]^CG[CT]C
BsmBI 4839 CGTCTC CGTCTC(1/5)
BspDI 4390 ATCGAT AT^CGAT
BspMI 340 ACCTGC ACCTGC(4/8)
BstEII 3013 GGTAACC G^GT[ACGT]ACC
BstPI 3013 GGTAACC G^GT[ACGT]ACC
Bsu36I 3879 CCTTAGG CC^T[ACGT]AGG
ClaI 4390 ATCGAT AT^CGAT
DraII 4723 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1141 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 146 CCGTGG C^C[GA][CT]GG
Eam1105I 4751 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3879 CCTTAGG CC^T[ACGT]AGG
EcoNI 3003 CCTTAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 4723 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3013 GGTAACC G^GT[ACGT]ACC
FbaI 1422 TGATCA T^GATCA
HgaI 4838 GCGTC GACGC(5/10)
Hin1I 1349 GACGTC G[GA]^CG[CT]C
HincII 2171 GTCGAC GT[CT]^[GA]AC
HindII 2171 GTCGAC GT[CT]^[GA]AC
MamI 3714 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3842 CAATTG C^AATTG
MspA1I 1834 CAGCTG C[AC]G^C[GT]G
MunI 3842 CAATTG C^AATTG
NaeI 101 GCCGGC GCC^GGC
NgoMIV 101 GCCGGC G^CCGGC
PaeR7I 1727 CTCGAG C^TCGAG
PflFI 4913 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3432 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1834 CAGCTG CAG^CTG
SalI 2171 GTCGAC G^TCGAC
SapI 4772 GCTCTTC GCTCTTC(1/4)
SmaI 96 CCCGGG CCC^GGG
SnaBI 386 TACGTA TAC^GTA
StuI 2262 AGGCCT AGG^CCT
Tth111I 4913 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2376 CCACATGCTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1727 CTCGAG C^TCGAG
XmaCI 96 CCCGGG C^CCGGG
XmaI 96 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2171 GTCGAC GT^[AC][GT]AC
4236 GTCTAC
AlwNI 868 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
3098 CAGAACCTG
AvaI 96 CCCGGG C^[CT]CG[GA]G
1727 CTCGAG
AvrII 357 CCTAGG C^CTAGG
4936 CCTAGG
BglI 46 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4805 GCCACTGTGGC
BglII 276 AGATCT A^GATCT
4680 AGATCT
BlnI 357 CCTAGG C^CTAGG
4936 CCTAGG
BsoBI 96 CCCGGG C^[CT]CG[GA]G
1727 CTCGAG
Bsp1407I 1697 TGTACA T^GTACA
2496 TGTACA
BsrFI 101 GCCGGC [GA]^CCGG[CT]
2248 ACCGGC
BsrGI 1697 TGTACA T^GTACA
2496 TGTACA
BssSI 1478 CACGAG CACGAG(-5/-1)
2969 CTCGTG
BstBI 287 TTCGAA TT^CGAA
4644 TTCGAA
Cfr10I 101 GCCGGC [GA]^CCGG[CT]
2248 ACCGGC
EcoRI 3075 GAATTC G^AATTC
3885 GAATTC
EcoT22I 830 ATGCAT ATGCA^T
4310 ATGCAT
NdeI 833 CATATG CA^TATG
3158 CATATG
NsiI 830 ATGCAT ATGCA^T
4310 ATGCAT
NspV 287 TTCGAA TT^CGAA
4644 TTCGAA
PflMI 1830 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3444 CCAGGCATTGG
PstI 1225 CTGCAG CTGCA^G
1615 CTGCAG
SacI 2756 GAGCTC GAGCT^C
3818 GAGCTC
SfuI 287 TTCGAA TT^CGAA
4644 TTCGAA
SspBI 1697 TGTACA T^GTACA
2496 TGTACA
SspI 4594 AATATT AAT^ATT
4998 AATATT
Van91I 1830 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3444 CCAGGCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BalI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspLU11I
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI